Journal ArticleDOI
Studies on the molecular weight and cryoprotective properties of polyvinylpyrrolidone and dextran with bacteria and erythrocytes.
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The distribution of molecules in various commercial samples of polyvinylpyrrolidone was studied by gel-exclusion chromatography on Sephadex columns and there was a trend toward greater cryoprotective activity with higher molecular weight.About:
This article is published in Cryobiology.The article was published on 1971-10-01. It has received 70 citations till now. The article focuses on the topics: Sephadex.read more
Citations
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Protectants used in the cryopreservation of microorganisms
TL;DR: Pairwise comparisons of the cryoprotective activity of the more common CPAs used in cryomicrobiology indicate that the most successful CPAs have been Me2SO, methanol, ethylene glycol, propylene glyCol, and serum or serum albumin, while glycerol, polyethylene gly col, PVP, and sucrose are less successful, and other sugars are the least effective.
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The effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing.
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A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos.
Susan J. Nixon,Richard I. Webb,Matthias Floetenmeyer,Nicole L. Schieber,Harriet P. Lo,Robert G. Parton +5 more
TL;DR: This study shows that in the absence of primary chemical fixation, excellent ultrastructure, preservation of green fluorescent protein (GFP) fluorescence, immunogold labelling and electron tomography can be obtained using a single technique involving high‐pressure freezing and embedding in Lowicryl resins at low temperature.
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Polymer cryoprotectants in the preservation of biological ultrastructure. I. Low temperature states of aqueous solutions of hydrophilic polymers.
TL;DR: The solid states formed by vitrified and frozen aqueous solutions of some hydrophilic polymers, able to act as biological cryoprotectants, have been studied by differential scanning calorimetry and freeze fracture electron microscopy.
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Freeze-injury in bacteria
Bibek Ray,Marvin L. Speck +1 more
TL;DR: The effects of freezing on bacterial cells have been studied essentially for three purposes: to understand the underlying physical and chemical changes that occur during freezing of biological materials, to elucidate the possible changes brought about by freezing and thawing of bacterial cells, and to develop means for successful freezing of higher forms of cells.
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Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
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Polyvinyl pyrrolidone as a plasma expander; studies on its excretion, distribution and metabolism.
TL;DR: Pyrrolidone was proposed in 1940 as an effective nontoxic plasma substitute and was administered to large numbers of soldiers and civilians during the ensuing war years in Germany and since then in other European countries and in the United States.
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Low Temperature Preservation of Mouse Lymphocytes with Dimethyl Sulfoxide
TL;DR: Mouse lymphocytes have been successfully preserved for 3 months at -196 C. in the presence of 15 per cent dimethyl sulfoxide and the ability of these lymphocytes to produce splenomegaly when injected with F1 hybrids was assessed.
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Mode of Protection with Polyvinylpyrrolidone in Freezing of Bone Marrow
Maxim Persidsky,Victor Richards +1 more
TL;DR: To determine whether the mechanism of this protection involves pinocytosis, hence intracellular penetration, or whether the protective action is extracellular or both extra- and intra-cellular is the object of this investigation.