Studies on the regulation of avidity at the level of the single antibody-forming cell the effect of antigen dose and time after immunization
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The hemolytic plaque formation of cells producing antibody against heterologous albumins was tested for sensitivity to specific inhibition by free antigen, and inhibition characteristics of plaques in this system were found to be a measure for the avidity of the antibody produced by the plaque-forming cells.Abstract:
The hemolytic plaque formation of cells producing antibody against heterologous albumins was tested for sensitivity to specific inhibition by free antigen. The inhibition characteristics of plaques in this system were found to be a measure for the avidity of the antibody produced by the plaque-forming cells (PFC:s). High avidity-producing PFC:s were more sensitive to inhibition than low avidity PFC:s. Immunization with a high dose of antigen induced PFC:s that produced antibody with a lower avidity as compared to PFC:s from animals immunized with a low dose. The avidity was increased with time. Determinations of avidity at the serum level were also made, and the results were in agreement with the findings at the cellular level. The present method made it possible to demonstrate differences in avidity of antibody at the level of the single antibody-forming cell. It may also constitute a useful tool for the analysis of the cellular events leading to the production of antibodies with varying affinities during the immune response.read more
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Membrane immunoglobulins and antigen receptors on B and T lymphocytes.
TL;DR: There are several qualitative and quantitative distinctions between T-cell and B-cell M-Ig that may explain many of the apparent differences in reactivity of these cells without requiring the existence of a different set of V genes or non-IG receptors in T cells.
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Plaque Forming Cells: Methodology and Theory
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Immunological studies of aging. II. Loss of IgG and high avidity plaque-forming cells and increased suppressor cell activity in aging mice.
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Immunological studies of aging. IV. The contribution of thymic involution to the immune deficiencies of aging mice and reversal with thymopoietin32-36.
TL;DR: The data suggest that a decline in thymic function that occurs during aging may contribute to the immunological deficiencies of old animals.
References
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Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
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TL;DR: Most qualitative and quantitative antibody tests measure secondary effects of primary antigen-antibody interactions, but they do not always reflect the total antibody content of the antiserum with respect to the test antigen.
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A Modification of the Hemolytic Plaque Assay for Use with Protein Antigens
TL;DR: The method is simple and sensitive and the results mimic the kinetics of the response that is seen in in vivo assays of serum antibody.
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Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells.
TL;DR: A method was described for the sensitization of erythrocytes with purified type-specific pneumococcal polysaccharide antigens using chromium chloride as a coupling agent to be used in routine passive hemagglutination and hemolysis tests.