Journal ArticleDOI
Studies on the structure-activity relationship and interaction mechanism of flavonoids and xanthine oxidase through enzyme kinetics, spectroscopy methods and molecular simulations
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TLDR
It is suggested that active flavonoids could bind to the active center of XO, prevent the entrance of substrate, and induce the rearrangement and conformation change of its secondary structures, ultimately resulting in the significant inhibition effect.About:
This article is published in Food Chemistry.The article was published on 2020-09-01. It has received 67 citations till now. The article focuses on the topics: Xanthine oxidase & Circular dichroism.read more
Citations
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Comparing the inhibitory abilities of epigallocatechin-3-gallate and gallocatechin gallate against tyrosinase and their combined effects with kojic acid.
TL;DR: In this article, the inhibitory mechanism of epigallocatechin-3-gallate (EGCG) and gallocatechin gallate (GCG) on tyrosinase was investigated.
Journal ArticleDOI
Studies on the interactions of theaflavin-3,3′-digallate with bovine serum albumin: Multi-spectroscopic analysis and molecular docking
Liang Zeng,Nerea Zabaleta +1 more
TL;DR: In this paper , the interaction between bovine serum albumin (BSA) and theaflavin-3,3′-digallate (TFDG), an important component in tea cream and functional substance of black tea, was investigated by fluorescence spectroscopy.
Journal ArticleDOI
Studies on the interactions of theaflavin-3,3'-digallate with bovine serum albumin: Multi-spectroscopic analysis and molecular docking
Liang Zeng,Xia Yu,Xinghong Cai,Nerea Zabaleta,Shuang Li,Liyong Luo,Jie Wang,Min Wang,Liang Zeng +8 more
TL;DR: In this article, the interaction between bovine serum albumin (BSA) and theaflavin-3,3'-digallate (TFDG), an important component in tea cream and functional substance of black tea, was investigated by fluorescence spectroscopy.
Journal ArticleDOI
Investigation of the interaction between Chrysoeriol and xanthine oxidase using computational and in vitro approaches.
TL;DR: In this paper, a comprehensive analysis of the interaction between CHE and XO was accomplished by enzyme kinetics, isothermal titration calorimetry (ITC), multi-spectroscopic methods, molecular simulation and ADMET.
Journal ArticleDOI
Epicatechin Gallate as Xanthine Oxidase Inhibitor: Inhibitory Kinetics, Binding Characteristics, Synergistic Inhibition, and Action Mechanism
TL;DR: In this article, the inhibitory ability and molecular mechanism of ECG on XO were investigated systematically, and the stable ECG-XO complex was formed by hydrogen bonds and van der Waals forces, with the binding constant of the magnitude of 104 L mol−1.
References
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Journal ArticleDOI
Thermodynamics of protein association reactions: forces contributing to stability
Philip D. Ross,S. Subramanian +1 more
TL;DR: On the basis of the thermochemical behavior of small molecule interactions, it is concluded that the strengthening of hydrogen bonds in the past decade, a complete thermodynamic description of the self-association of many proteins and their interactions is concluded.
Journal ArticleDOI
Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships.
TL;DR: The diversity and multiple mechanisms of flavonoid action, together with the numerous methods of initiation, detection and measurement of oxidative processes in vitro and in vivo offer plausible explanations for existing discrepancies in structure-activity relationships.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Therapeutic Effects of Xanthine Oxidase Inhibitors: Renaissance Half a Century after the Discovery of Allopurinol
TL;DR: A critical overview of the effects of XO inhibitors in various pathophysiological conditions is presented and the various emerging therapeutic strategies offered by this approach are reviewed.
Journal ArticleDOI
Structure−Activity Relationship and Classification of Flavonoids as Inhibitors of Xanthine Oxidase and Superoxide Scavengers
Paul Cos,Li Ying,M. Calomme,Jia P. Hu,K. Cimanga,Bart Van Poel,Luc Pieters,A. Vlietinck,Dirk Vanden Berghe +8 more
TL;DR: The structure-activity relationship of flavonoids as inhibitors of xanthine oxidase and as scavengers of the superoxide radical, produced by the action of the enzyme xanthines oxidase, was investigated and showed slightly higher inhibitory activity than flavonols.
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