Journal ArticleDOI
Surface Replicas for Use in the Electron Microscope
Vincent J. Schaefer,David Harker +1 more
TLDR
In this article, a simple method is described for making replicas of surfaces for study in the electron microscope, and the surface to be studied is cleaned and then coated with a very thin slightly wedge-shaped film of polyvinyl formal having a range in thickness from about 500 to 750A in 2.5 cm.Abstract:
A simple method is described for making replicas of surfaces for study in the electron microscope. The surface to be studied is cleaned and then coated with a very thin slightly wedge‐shaped film of polyvinyl formal having a range in thickness from about 500 to 750A in 2.5 cm. This film is formed by dipping the prepared surface into a 0.5‐percent solution of the resin dissolved in dioxane or ethylene dichloride and permitting the solvent to evaporate while the surface is held in a vertical position. The replica film is removed by placing the coated surface under water and peeling the film from it. A description of this technique is given in detail. A graph is presented which shows the thickness of resin films obtainable from various concentrations of resin in dioxane, and a table gives the interference effects which may be used for determining approximate thicknesses of thin resin films. Several easy procedures are described for checking the thickness of such films. A technique is described for preparing strong films having a thickness of 100A and an area of 3 cm2 when mounted in air. Such films are very useful when working with the electron microscope and several methods are described for mounting specimens on them, including a conditioning treatment—useful in mounting water suspensions—which makes one surface very hydrophilic. Five micrographs show the results obtainable with the techniques described.read more
Citations
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Journal ArticleDOI
From Micro- to Nanofabrication with Soft Materials
Stephen R. Quake,Axel Scherer +1 more
TL;DR: Active microfluidic devices for cell sorting and biochemical assays, replication-molded optics with subdiffraction limit features, and nanometer-scale resonators and wires formed from single-molecule DNA templates are discussed as examples of how the special properties of soft materials address outstanding problems in device fabrication.
Journal ArticleDOI
Applications of Metallic Shadow‐Casting to Microscopy
TL;DR: In this paper, the authors discussed the factors which determine image contrast in optical and electron micrographs and proposed a shadow casting technique whereby the contrast of images is greatly increased by depositing obliquely a thin film of metal on the microscope preparations.
Journal ArticleDOI
Colorimetric Microdetermination of Formic Acid Based on Reduction to Formaldehyde
Journal ArticleDOI
Relationship of growth cessation with the formation of diferulate cross-links and p-coumaroylated lignins in tall fescue leaf blades.
TL;DR: Relationships among cell wall feruloylation, diferulate cross-linking, p-coumarate deposition, and apoplastic peroxidase activity with changes in the elongation rate of leaf blades of slow and rapid elongating genotypes of tall fescue were examined.
Journal ArticleDOI
A NEW MATERIAL AND TECHNIQUES FOR THE FABRICATION AND MEASUREMENT OF VERY THIN FILMS FOR USE IN 4π-COUNTING
B. D. Pate,L. Yaffe +1 more
TL;DR: In this paper, a method for preparing thin films for use in beta-spectrometry and 4π-counting is described, made of polyvinylchloride-acetate copolymer.
References
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Journal ArticleDOI
Properties and Structure of Protein Monolayers.
Journal ArticleDOI
Electron Diffraction Studies of Thin Films. I. Structure of Very Thin Films
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