Journal ArticleDOI
Synthesis of functional protein in liposome.
Wei Yu,Kanetomo Sato,Maki Wakabayashi,Tomoyuki Nakaishi,Elizabeth P. Ko-Mitamura,Yasufumi Shima,Itaru Urabe,Tetsuya Yomo,Tetsuya Yomo +8 more
TLDR
The Liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture and the synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from theliposome on flow cytometry analysis and fluorescence microscopy.About:
This article is published in Journal of Bioscience and Bioengineering.The article was published on 2001-01-01. It has received 222 citations till now. The article focuses on the topics: Liposome & Cell-free protein synthesis.read more
Citations
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Journal ArticleDOI
Self-Assembled Nanoreactors
Dennis M. Vriezema,Marta Comellas Aragones,Johannes A. A. W. Elemans,Jeroen J. L. M. Cornelissen,Alan E. Rowan,Roeland J. M. Nolte +5 more
TL;DR: An overview of the wide range of nanoreactors that have been constructed from synthetic and biological building blocks using both covalent and noncovalent approaches is given, starting from small organic molecular containers expanding to large compartment-containing assemblies.
Journal ArticleDOI
Prebiotic systems chemistry: new perspectives for the origins of life.
TL;DR: This paper presents a probabilistic analysis of the stationary phase replacement of Na6(CO3)(SO4)/ Na2SO4 in horseshoe clusters and shows clear trends in the number of stationary phases and in the stationary phases of Na2CO3.
Book
The Emergence of Life: From Chemical Origins to Synthetic Biology
TL;DR: The conceptual framework of the research on the origin of life on Earth includes approaches to the definitions of life, self-organization, autopoiesis, and the logic of cellular life.
Journal ArticleDOI
Approaches to semi-synthetic minimal cells: a review
TL;DR: A synthetic review on the minimal living cell, defined as an artificial or a semi-artificial cell having the minimal and sufficient number of components to be considered alive, and describes concepts and experiments based on these constructions.
Journal ArticleDOI
Gene expression within cell-sized lipid vesicles.
Shin Ichiro M. Nomura,Kanta Tsumoto,Tsutomu Hamada,Kazunari Akiyoshi,Yoichi Nakatani,Kenichi Yoshikawa +5 more
TL;DR: The synthesized rsGFP in individual vesicles is safe from attack by proteinase K added to the external aqueous solution, and will be useful for practical applications, such as the construction of microreactors.
References
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PatentDOI
FACS-optimized mutants of the green fluorescent protein (GFP)
TL;DR: In this article, three classes of GFP mutants having single excitation maxima around 488 nm are shown to be brighter than wild-type GFP following 488-nm excitation.
Journal ArticleDOI
Sterically stabilized liposomes
Martin C. Woodle,Danilo D. Lasic +1 more
TL;DR: The structure-function relationship of PEG-derivatized phosphatidylethanolamine (PEG-PE) has been examined by measurement of blood lifetime and tissue distribution in both mice and rats and Steric stabilization has been proposed as a theoretical basis for the results.
Journal ArticleDOI
Man-made cell-like compartments for molecular evolution
TL;DR: It is demonstrated the linkage of genotype to phenotype in man-made compartments using a model system and a selection for target-specific DNA methylation was based on the resistance of the product (methylated DNA) to restriction digestion.
Journal Article
Synthesizing life : Paths to unforeseeable science & technology
TL;DR: Advances in directed evolution and membrane biophysics make the synthesis of simple living cells, if not yet foreseeable reality, an imaginable goal.
Journal ArticleDOI
In vitro synthesis of protein in microbial systems.
TL;DR: This review is devoted to studies involving synthesis of complete proteins in cell-free systems, and a number of laboratories have studied the DNA-dependent RNA directed synthesis of both viral and bacterial proteins in E. coli extracts.