Journal ArticleDOI
Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication.
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TLDR
Immunofluorescence studies reveal that there are dramatic changes in the nuclear distribution of cyclin during S phase and that these depend on DNA synthesis or events during Sphase, strengthening the notion that cyclin is an important component of the events leading to DNA replication.About:
This article is published in Experimental Cell Research.The article was published on 1986-04-01. It has received 243 citations till now. The article focuses on the topics: Cyclin E & Cyclin D.read more
Citations
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Journal ArticleDOI
Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
TL;DR: Cyclin and the auxiliary protein of DNA polymerase-δ are identical, and it is reported here that these two are identical.
Journal ArticleDOI
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein
Gregory Prelich,Cheng-Keat Tan,Matthew Kostura,Michael B. Mathews,Antero G. So,Kathleen M. Downey,Bruce Stillman +6 more
TL;DR: It is shown that PCNA and the polyrnerase-δ auxiliary protein have similar electrophoretic behaviour and are both recognized by anti-PCNA human autoantibodies, and both proteins are functionally equivalent; they stimulate SV40 DNA replication in vitro and increase the processivity of calf thymus DNA polymerase- δ.
Journal ArticleDOI
Dynamic Changes of BRCA1 Subnuclear Location and Phosphorylation State Are Initiated by DNA Damage
Ralph Scully,Junjie Chen,Robert L. Ochs,Kathleen S. Keegan,Merl F. Hoekstra,Jean Feunteun,David M. Livingston +6 more
TL;DR: The data imply that the BRCA1 S phase foci are dynamic physiological elements, responsive to DNA damage, and that B RCA1-containing multiprotein complexes participate in a replication checkpoint response.
Journal ArticleDOI
Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.
R Bravo,H Macdonald-Bravo +1 more
TL;DR: It was shown that the staining patterns of the replicon clusters and their order of appearance throughout the S phase are identical to those observed for cyclin, demonstrating that cyclin is tightly associated to the sites of DNA replication and that it must have a fundamental role in DNA synthesis in eukaryotic cells.
Journal ArticleDOI
Dynamic organization of DNA replication in mammalian cell nuclei: spatially and temporally defined replication of chromosome-specific alpha-satellite DNA sequences.
TL;DR: The data demonstrates that specific DNA sequences replicate at spatially and temporally defined points during the cell cycle and supports a spatially dynamic model of DNA replication.
References
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Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Book ChapterDOI
Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine.
TL;DR: Autoantibodies to nuclear antigens (ANAs) have assumed an important place in the diagnostic armamentarium of the clinician because of distinct profiles of ANAs in different diseases.
Journal Article
Autoantibody to a Nuclear Antigen in Proliferating Cells
TL;DR: The identification of an autoantibody in the sera of some patients with systemic lupus erythematosus that reacted with nuclear antigen(s) of proliferating cells that might serve as useful biologic markers to study stimulated lymphocytes and other proliferation cells.
Journal ArticleDOI
Identity of the proliferating cell nuclear antigen and cyclin.
TL;DR: It is established here that PCNA and cyclin are identical, and it is shown thatPCNA is an acidic nuclear protein of apparent molecular weight 35,000, which is similar to ‘cyclin’.
Journal ArticleDOI
A search for differential polypeptide synthesis throughout the cell cycle of hela cells
Rodrigo Bravo,Julio E. Celis +1 more
TL;DR: Quantitation of 99 abundant polypeptides (acidic and basic) in pulse- labeled and long-term labeled cells revealed that the relative amount (i.e., the rate of synthesis) of most polypePTides remains constant throughout the cell cycle.
Related Papers (5)
Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.
R Bravo,H Macdonald-Bravo +1 more