Journal ArticleDOI
T-lymphocyte priming and protection against Friend leukemia by vaccinia-retrovirus env gene recombinant.
TLDR
Mice inoculated with live recombinant vaccinia virus had an envelope-specific T-cell proliferative response and, after challenge with Friend virus complex, developed neutralizing antibody and cytotoxic T cells (CTL) and were protected against leukemia.Abstract:Â
The current prevalence of the acquired immune deficiency syndrome in humans has provoked renewed interest in methods of protective immunization against retrovirus-induced diseases. In this study, a vaccinia-retrovirus recombinant vector was constructed to study mechanisms of immune protection against Friend virus leukemia in mice. The envelope (env) gene from Friend murine leukemia virus (F-MuLV) was inserted into the genome of a vaccinia virus expression vector. Infected cells synthesized gp85, the glycosylated primary product of the env gene. Processing to gp70 and p15E, and cell surface localization, were similar to that occurring in cells infected with F-MuLV. Mice inoculated with live recombinant vaccinia virus had an envelope-specific T-cell proliferative response and, after challenge with Friend virus complex, developed neutralizing antibody and cytotoxic T cells (CTL) and were protected against leukemia. In contrast, unimmunized and control groups developed a delayed neutralizing antibody response, but no detectable CTL, and succumbed to leukemia. Genes of the major histocompatibility complex influenced protection induced by the vaccinia recombinant but not that induced by attenuated N-tropic Friend virus.read more
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Book ChapterDOI
Adoptive T cell therapy of tumors: mechanisms operative in the recognition and elimination of tumor cells.
TL;DR: This chapter attempts to identify the principles and issues elucidated in animal models that may provide insights for the development of effective approaches to modulate T cell functions to promote the eradication of human tumors.
Journal ArticleDOI
Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety
TL;DR: A genetically altered vaccinia virus that is unable to replicate in mammalian cells and produces diminished cytopathic effects retains the capacity for high-level gene expression and immunogenicity while promising exceptional safety for laboratory workers and potential vaccine recipients.
Journal ArticleDOI
Vaccinia virus: a tool for research and vaccine development
TL;DR: Rec recombinant vaccinia virus is no longer needed for smallpox immunization, but now serves as a useful vector for expressing genes within the cytoplasm of eukaryotic cells.
Journal ArticleDOI
Safety of and immunological response to a recombinant vaccinia virus vaccine expressing HIV envelope glycoprotein
Elizabeth Cooney,Elizabeth Cooney,Elizabeth Cooney,Ann C. Collier,Ann C. Collier,Ann C. Collier,Philip D. Greenberg,Philip D. Greenberg,Philip D. Greenberg,Robert W. Coombs,Robert W. Coombs,Robert W. Coombs,J. Zarling,J. Zarling,J. Zarling,D. E. Arditti,D. E. Arditti,D. E. Arditti,M. C. Hoffman,M. C. Hoffman,M. C. Hoffman,Shiu Lok Hu,Shiu Lok Hu,Shiu Lok Hu,Lawrence Corey,Lawrence Corey,Lawrence Corey +26 more
TL;DR: The 2 vaccinia-naive subjects vaccinated with HIVAC-1e showed strong T-cell responses to homologous and heterologous strains of whole virus and to recombinant gp160 protein that remained detectable for over a year; antibodies to HIV envelope also developed in both.
Book ChapterDOI
Retrovirus envelope glycoproteins
Eric Hunter,Ronald Swanstrom +1 more
TL;DR: The envelope glycoprotein complex of replication competent retroviruses is comprised of two polypeptides, an external, glycosylated, hydrophilicpolypeptide (SU) and a membrane-spanning protein (TM), that form a knob or knobbed spike on the surface of the virion.
References
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Die Molekularbiologie der Tumorviren
TL;DR: All classes of vertebrates harbor tumor viruses that are capable of inducing either tumors or leukemias, and these viruses have already been assigned to specific regions of their DNA.
Journal ArticleDOI
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Frank Lilly,Theodore Pincus +1 more
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Journal ArticleDOI
Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene
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TL;DR: V-RGpro8 virus was highly effective in priming mice to generate a secondary rabies virus-specific cytotoxic T-lymphocyte response following culture of lymphocytes with either ERA or PM strains of rabiesirus.
Journal ArticleDOI
Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
TL;DR: Rabbits and hamsters inoculated intradermally with recombinant virus produced circulating antibodies that inhibited hemagglutination by influenza virus and vaccinated hamsters achieved levels of antibody similar to those obtained upon primary infection with influenza virus.