Journal ArticleDOI
Temperature gradients in preparative high-performance liquid chromatography columns
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TLDR
In this article, temperature profiles measured in a preparative LC column of 60 mm diameter as a function of the packing material, the eluent flow-rate and the ELUent composition are presented.About:
This article is published in Journal of Chromatography A.The article was published on 1997-05-02. It has received 49 citations till now. The article focuses on the topics: Van Deemter equation & Packed bed.read more
Citations
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Journal ArticleDOI
Control of column temperature in reversed-phase liquid chromatography
TL;DR: In the present study, various temperature-related problems which can result in a failure of method transfer for non-ambient RP-LC methods were examined and means for correcting for such effects and thereby ensuring method transferability are described.
Journal ArticleDOI
Experimental studies of pressure/temperature dependence of protein adsorption equilibrium in reversed-phase high-performance liquid chromatography
Paweł Szabelski,Alberto Cavazzini,Alberto Cavazzini,K. Kaczmarski,K. Kaczmarski,Xiaoda Liu,Xiaoda Liu,J Van Horn,Georges Guiochon,Georges Guiochon +9 more
TL;DR: The results indicate that pressure and temperature affect the retention behavior of insulins in a different and separate way.
Journal ArticleDOI
Modeling of thermal processes in high pressure liquid chromatography II. Thermal heterogeneity at very high pressures
Krzysztof Kaczmarski,Fabrice Gritti,Fabrice Gritti,Joanna Kostka,Georges Guiochon,Georges Guiochon +5 more
TL;DR: A model combining the heat and mass balance of the column, the equations of flow through porous media, and a linear isotherm model of the analyte is solved and validated for conventional columns operated under moderate pressures and results prove that this model accounts well for all the experimental results.
Journal ArticleDOI
Detection Modes with High Temperature Liquid Chromatography—A Review
D. Guillarme,S. Heinisch +1 more
TL;DR: The use of thermally resistant stationary phases based on porous graphitic carbon, zircon, titania, or organic polymers allows the use of high temperatures in liquid chromatography (HTLC) without any degradation of the stationary phase as mentioned in this paper.
Journal ArticleDOI
Thermal effects in reactive liquid chromatography
Tuomo Sainio,Malte Kaspereit,Achim Kienle,Achim Kienle,Andreas Seidel-Morgenstern,Andreas Seidel-Morgenstern +5 more
TL;DR: In this paper, a self-amplifying positive thermal wave was found to develop in an exothermic esterification reaction catalyzed by an acidic ion-exchange resin.
References
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Journal ArticleDOI
Band spreading in liquid chromatography
Csaba Horva´th,Hung-Jye Lin +1 more
TL;DR: In this paper, a general plate height equation has been derived to express the effect of axial dispersion in the interstitial space, mass transfer resistances at the boundary and in the interior of porous stationary phase particles, and kinetic resistances associated with the reversible binding of eluite by the stationary phase.
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Ultimate limits in high-pressure liquid chromatography☆
TL;DR: In this paper, the authors show that the temperature of an eluent may increase by up to 35° if Δp = 500 atm and the capacity ratio of a sample decreases with increasing Δp.
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The role of the temperature in reversed-phase high-performance liquid chromatography using pyrocarbon-containing adsorbents
TL;DR: In this article, the effect of temperature in liquid chromatography on pyrocarbon-containing adsorbents is considered in terms of heats of transfer, solvent eluotropic strength, column linear capacities, pressre drops and efficiencies.
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Temperature gradients in HPLC columns due to viscous heat dissipation
TL;DR: In this paper, the authors examined the effect of viscous heat dissipation on HPLC columns and concluded that viscous temperature dissipation constitutes an obstacle to obtaining higher speed and efficiency in HPLC by using smaller particles.
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Mobile phase effects in reversed-phase chromatography
TL;DR: In this article, the free energy change associated with the chromatographic process asd a function of both the eluent composition and temperature was analyzed. And the authors proposed a three-parameter equation to transform retention data for a solute obtained at a given temperature and solvent composition to another set of experimental conditions, which revealed the existence of a noncompensating enthalpy residue in the absence of heat capacity effects.