The determination of 2:3-diketo-l-gulonic acid.
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This article is published in Biochemical Journal.The article was published on 1943-04-01 and is currently open access. It has received 28 citations till now. The article focuses on the topics: Ascorbic acid.read more
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The determination of diketo l-gulonic acid, dehydro-l-ascorbic acid, and l-ascorbic acid in the same tissue extract by the 2, 4-dinitrophenylhydrazine method.
TL;DR: Methods for determinations of vitamin C dehydro-Z-ascorbic (DHA), DHA, and DKA in the presence of each other are reported, indicating that these derivatives are identical substances.
Journal ArticleDOI
A rapid, simple method for the determination of reduced, dehydro-, and total ascorbic acid in biological material.
TL;DR: The determination of “free” and “bound” ascorbic acid in liver tissue has been reported by Sumerwell and Sealock and Geschwind, Williams, and Li obtained good recoveries of l-ascorbic acid added to adrenal glands, using higher temperatures of incubation with 2,4-dinitrophenylhydrazine.
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Specific determination of ascorbic acid with chemical derivatization and high-performance liquid chromatography
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The oxidation of dehydroascorbic acid and 2,3-diketogulonate by distinct reactive oxygen species.
TL;DR: DHA and DKG yield different oxidation products when attacked by different ROS, enabling organisms to respond appropriately to diverse stresses.
References
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Studies on the metabolism of pyruvic acid in normal and vitamin B(1)-deficient states: A rapid, specific and sensitive method for the estimation of blood pyruvate.
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Ascorbic acid as the antiscorbutic factor.
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The chemical behaviour of dehydro-l-ascorbic acid in vitro and in vivo.
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
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