Journal ArticleDOI
The mode of action of some benzimidazole drugs on Trichinella spiralis.
TLDR
Measurements of free glucose, glycogen reserves and soluble protein in the treated larvae indicate that in vivo, mebendazole and thiabendazoles clearly diminish free glucose levels, although in vitro only mebENDazole produces the same diminution.Abstract:
In an attempt to find possible targets for benzimidazole action in muscle-stage larvae of Trichinella spiralis, the effects of mebendazole and thiabendazole were tested in vivo by oral treatment of infested mice and in vitro by including these anthelmintics in an adequate maintenance medium containing decapsulated larvae. The effects of the anthelmintics on succinate dehydrogenase and fumarate reductase activities, measured in the mitochondrial fraction obtained from the in vivo- or in vitro-treated larvae showed that only thiabendazole causes significant inhibition of fumarate reductase activity. On the other hand, measurements of free glucose, glycogen reserves and soluble protein in the treated larvae indicate that in vivo, mebendazole and thiabendazole clearly diminish free glucose levels, although in vitro only mebendazole produces the same diminution. Both the glycogen and protein contents of the larvae remained unchanged after treatment in vivo or in vitro. The importance of these findings with regard to a possible site of action for mebendazole and thiabendazole is discussed.read more
Citations
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Journal ArticleDOI
Mebendazole induces apoptosis via Bcl-2 inactivation in chemoresistant melanoma cells.
TL;DR: It is revealed that mebendazole inhibits melanoma growth with an average IC50 of 0.32 μmol/L and preferentially induces apoptosis in melanoma cells compared with melanocytes, and is identified as a potent, melanoma-specific cytotoxic agent.
Journal ArticleDOI
Fumarate metabolism and the microaerophily of Campylobacter species
TL;DR: The results suggested that Frd and Fum are constitutive and play a fundamental role in these microaerophiles which show characteristics of anaerobic metabolism, and that the Frd inhibitors tested would not be of therapeutic use.
Journal ArticleDOI
Comparison of in vitro and in vivo acute fish toxicity in relation to toxicant mode of action.
TL;DR: Results indicate that limitation of the neutral red assay in predicting acute fish toxicity is indicated and a promising alternative might be the assessment of toxicity in a set of in vitro systems addressing also cell-specific functions which are related to the mode of toxic action of the compound.
Journal ArticleDOI
Effect of thiabendazole and fenbendazole on glucose uptake and carbohydrate metabolism in Trichuris globulosa.
TL;DR: In this paper, a non-linear function of time and limiting substrate concentration of T. globulosa was found to possess the full complement of enzyme systems of glycolysis, Krebs cycle, phosphatases, transaminases and other NAD-and NADPdependent enzymes.
Journal ArticleDOI
Preliminary characterization and interaction of tubulin from Trichinella spiralis larvae with benzimidazole derivatives.
A. Jimenez-Gonzalez,C. de Armas-Serra,A. Criado-Fornelio,N. Casado-Escribano,Filomena Rodriguez-Caabeiro,J.C. Díez +5 more
TL;DR: Tubulin was estimated to account for 0.3% of the total soluble protein in Trichinella spiralis cytosolic fractions and electrophoretic mobility of T. spiralis tubulin in sodium dodecyl sulphate-polyacrylamide gels was very similar to that shown by pig brain tubulin.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor
TL;DR: It was found that the system of phenol and 4-amino phenazone is well suited to the determination of glucose and the development of phosphatase is described.
Book
Trichinella and Trichinosis
TL;DR: This review focuses on the part of the Gastrointestinal Phase of the Larva-Parasite Interrelationships that concerns Migration of the First-Stage Larva to the Niche and the Influence of Lamina Propria.
Journal ArticleDOI
Enzymatic microdetermination of glycogen.
Michael L. Bruss,Arthur L. Black +1 more
TL;DR: The ABTS method was found to be three times as sensitive as the older o -dianisidine method and correlated highly with those obtained by acid hydrolysis for rabbit liver glycogen.