Journal ArticleDOI
The occurrence of free and bound cytokinins in plasmodia of Plasmodiophora brassicae isolated from tissue cultures of clubroots.
Reads0
Chats0
TLDR
Cytokinins were extracted from plasmodia and from the remaining host cytoplasm and the different glucosides were determined after treatment of the water fraction with β-glucosidase.Abstract:
Plasmodia have been isolated from Brassica campestris tissue infected with Plasmodiophora brassicae. Cytokinins were extracted from plasmodia and from the remaining host cytoplasm. Fractions were separated in a butanol soluble fraction containing free cytokinins and a water soluble fraction containing bound forms, presumably glucose-6-phosphate derivatives of zeatin and zeatin riboside. The butanol fraction was analysed by high pressure liquid chromatography (HPLC). The different glucosides were determined after treatment of the water fraction with β-glucosidase.read more
Citations
More filters
Journal ArticleDOI
Transcriptome analysis of Arabidopsis clubroots indicate a key role for cytokinins in disease development.
Johannes Siemens,Ingo Keller,Johannes Sarx,Sabine Kunz,Astrid Schuller,Wolfgang E. Nagel,Thomas Schmülling,Martin Parniske,Jutta Ludwig-Müller +8 more
TL;DR: Host gene expression during the development of the clubroot disease was investigated using the Arabidopsis ATH1 genome array and cytokinin oxidase/dehydrogenase overexpressing lines were disease resistant, clearly indicating the importance of cytkinin as a key factor in club root disease development.
Journal ArticleDOI
Induction of trehalase in Arabidopsis plants infected with the trehalose-producing pathogen Plasmodiophora brassicae.
David Brodmann,Astrid Schuller,Jutta Ludwig-Müller,Roger A. Aeschbacher,Andres Wiemken,Thomas Boller,Astrid Wingler +6 more
TL;DR: It is found that trehalose accumulated strongly in the infected organs and, to a lesser extent, in the leaves and stems of infected plants, and it is unlikely that tre Halase was induced by its substrate, but the induction of trehalase may be part of the plant's defense response and may prevent excess accumulation in the plant cells, where it could interfere with the regulation of carbon metabolism.
Journal ArticleDOI
A Hormone and Proteome Approach to Picturing the Initial Metabolic Events During Plasmodiophora brassicae Infection on Arabidopsis
Sylvie Devos,Kris Laukens,Peter Deckers,Dominique Van Der Straeten,Tom Beeckman,Dirk Inzé,Harry Van Onckelen,Erwin Witters,Els Prinsen +8 more
TL;DR: It is postulate that, at the very first stages of Plasmodiophora infection, plasmodial-produced cytokinins trigger a local re-initiation of cell division in the root cortex, which acts as a sink for host-derived indole-3-acetic acid, carbohydrates, nitrogen, and energy to maintain the pathogen and to trigger gall development.
Journal ArticleDOI
Metabolism and Plant Hormone Action During Clubroot Disease
TL;DR: A model is presented for how primary metabolism and secondary metabolism, including plant hormones, interact to induce clubroot formation in Brassicaceae plants.
Journal ArticleDOI
Plasmodiophora brassicae : aspects of pathogenesis and resistance in Brassica oleracea
TL;DR: Clubroot is one of the most damaging diseases in Brassica oleracea crops world-wide and progress in the understanding of the inheritance of resistance is being made through the use of single-spore isolates of the pathogen, and theUse of molecular markers for resistance genes.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
The role of cytokinins in clubroot formation
H.M. Dekhuijzen,J.C. Overeem +1 more
TL;DR: Findings are consistent with the fact that explants infected with P. brassicae are independent of cytokinins and auxins for their growth, whereas callus of healthy turnip tissue requires these hormones for continued growth.
Journal ArticleDOI
The occurrence of free and bound cytokinins in clubroots and Plasmodiophora brassicae infected turnip tissue cultures
TL;DR: Evidence is presented for a novel cytokinin in the water soluble fraction which yields free zeatin and glucose-6-phosphate after treatment with β-glucosidase.
Journal ArticleDOI
The Recovery of Cytokinins during Extraction and Purification of Clubroot Tissue
H. M. Dekhuijzen,Ch. Th. Gevers +1 more
TL;DR: In this article, the authors determined the loss of one naturally occurring cytokinin (zeatin) and one synthetic cytokin (kinetin) during purification of turnips (Brassica compestris) infected by Plasmodiophora brassicae (clubroot).
Journal ArticleDOI
Biosynthesis of cytokinins by tobacco cell cultures
Noriko Nishinari,Kunihiko Syōno +1 more
Related Papers (5)
Isomers of zeatin and zeatin riboside in clubroot tissue: evidence for trans‐zeatin biosynthesis by Plasmodiophora brassicae
Peter Müller,Willy Hilgenberg +1 more