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The proliferative states of mouse granulopoietic progenitor cells.
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Mouse granulopoietic progenitor cells can be detected by their capacity to form colonies in culture (CFU-C), and the degree to which their colony-forming capacity was destroyed following exposure to a pulse of high specific-activity 3HTdR is studied.Abstract:
SummaryMouse granulopoietic progenitor cells can be detected by their capacity to form colonies in culture (CFU-C). The proliferative state of these cells was studied by determining the degree to which their colony-forming capacity was destroyed following exposure to a pulse of high specific-activity 3HTdR. When marrow was obtained from normal adult mice 35% of CFU-C were inactivated by this procedure. In contrast, 80% of CFU-C were inactivated when cell populations were obtained from regenerating bone marrow. These results are interpreted to mean that CFU-C in normal mice are partitioned into two populations, one proliferating rapidly and the other slowly or not at all. In regenerating marrow the partition is changed, with all or almost all of the cells proliferating rapidly.read more
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Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells.
TL;DR: Using 3H-thymidine labeling, a subpopulation of corneal epithelial basal cells are identified in the peripheral cornea in a region called limbus that are normally slow cycling, but can be stimulated to proliferate in response to wounding and to a tumor promotor, TPA.
Journal ArticleDOI
Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny
Hugo Castro-Malaspina,Hugo Castro-Malaspina,Hugo Castro-Malaspina,Gene Resnick,Gene Resnick,Gene Resnick,Neena Kapoor,Neena Kapoor,Neena Kapoor,Paul A. Meyers,Paul A. Meyers,Paul A. Meyers,Dominick R. Chiarieri,Dominick R. Chiarieri,Dominick R. Chiarieri,Susan Mckenzie,Susan Mckenzie,Susan Mckenzie,Hal E. Broxmeyer,Hal E. Broxmeyer,Hal E. Broxmeyer,Malcolm A.S. Moore,Malcolm A.S. Moore,Malcolm A.S. Moore +23 more
TL;DR: Analysis of CFU-F proliferative status by the thymidine suicide technique indicated that this cell was noncycling in individuals with undisturbed bone marrow function and data support the conclusion that the colonies described in this study are of fibroblastic nature.
Journal ArticleDOI
Primary Bioassay of Human Myeloma Stem Cells
TL;DR: An in vitro assay which permits formation of colonies of human monoclonal plasma cells in soft agar should prove useful in studying the in vitro biological behavior of certain bone marrow-derived (B)-cell neoplasia and systematic and predictive studies of anticancer drug effects on myeloma stem cells should now be feasible.
Journal ArticleDOI
Hematopoietic stem cells: concepts, definitions, and the new reality.
TL;DR: This work has provided definitive evidence of linearly transmitted heterogeneity in HSC states and anticipates the need and use of emerging new technologies to establish models that will accommodate such pluralistic features of HSCs and their control mechanisms.
Journal ArticleDOI
Ex vivo expansion of murine marrow cells with interleukin-3 (IL-3), IL- 6, IL-11, and stem cell factor leads to impaired engraftment in irradiated hosts
TL;DR: Data presented here from two different competitive transplant studies show a defect of cytokine expanded marrow related to cell cycle activation which manifests as defective long-term repopulating capability in irradiated host mice.
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Methods of Tissue Culture
TL;DR: Adventures in Respiration is not a physiological monograph in the usual sense, but rather the scientific autobiography of one of America's leaders in the physiology of respiration.