Journal ArticleDOI
The rapid resolution of naturally occurring amino acids by thin-layer chromatography
K. Jones,J.G. Heathcote +1 more
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Using a two-dimensional thin-layer chromatographic technique, with thin layers of cellulose powder, twenty-three naturally occurring amino acids have been separated unambiguously on a single chromatogram.About:
This article is published in Journal of Chromatography A.The article was published on 1966-01-01. It has received 108 citations till now. The article focuses on the topics: Amino acid & Thin-layer chromatography.read more
Citations
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Structure of the porcine vasoactive intestinal octacosapeptide. The amino-acid sequence. Use of kallikrein in its determination.
Viktor Mutt,Sami I. Said +1 more
TL;DR: The elucidation of the structure of the octacosapeptide was facilitated by the finding that pancreatic kallikrein preferentially cleaved only one of the three bonds in its N-terminal cyanogen bromide heptadecapeptide that are susceptible to cleavage with trypsin.
Journal ArticleDOI
Constitutive Inorganic Pyrophosphatase of Escherichia coli I. PURIFICATION AND CATALYTIC PROPERTIES
TL;DR: An intracellular, inorganic pyrophosphatase from Escherichia coli K-12 3000(λ) has been purified 500-fold to a state of apparent homogeneity suggesting stoichiometric combination with the substrate.
Journal ArticleDOI
Type-Specific Antigens of Group B Type Ic Streptococci
Hazel W. Wilkinson,R. G. Eagon +1 more
TL;DR: The type-specific antigens of group B type Ic (old designation type Ii) streptococci were extracted, purified, and characterized by serological and chemical methods, indicating that the immunodominant group of this antigen consists of more than a simple monosaccharide.
Journal ArticleDOI
Purification and Properties of Alanine Dehydrogenase from Bacillus sphaericus
Toshihisa Ohashima,Kenji Soda +1 more
TL;DR: The bacterial distribution of alanine dehydrogenase (L-alanine:NAD+ oxidoreductase, deaminating, EC 1.1.1) was investigated, and high activity was found in Bacillus species, and the enzyme is stable over a wide pH range.
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Feeding by a “nonfeeding” larva: uptake of dissolved amino acids from seawater by lecithotrophic larvae of the gastropod Haliotis rufescens
W. B. Jaeckle,Donal T. Manahan +1 more
TL;DR: These lecithotrophic larvae are not energetically independent of their environment, a result which differs from the current view of energy allocation to “nonfeeding” larvae.
References
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Journal ArticleDOI
Dünnschicht-Chromatographie von Aminosäuren
M. Brenner,A. Niederwieser +1 more
TL;DR: Amino acid separation with six different solvents on chromatoplates prepared from Kieselgel G (Merck) is described in this article, which is equal or superior to paper chromatography.
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Eine Multidimensionale technik zur chromatographischen identifizierung von aminosäuren
E. von Arx,R. Neher +1 more
TL;DR: A novel multidimensional technique has been elaborated and found suitable for the chromatographic identification of 52 amino acids and the relative merits of cellulose and silicagel as carriers are discussed in relation to separation efficiency, reproducibility and sensitivity of detection.
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Dünnschicht-Chromatographie von Aminosäuren auf Kieselgel G. 2. Mitteilung. Eine Schnellmethode zur Trennung und zum qualitativen Nachweis von 22 Aminosäuren
TL;DR: In this article, a thin layer chromatographic method for rapid separation and detection of 22 commonly occurring amino-acids is described, with special reference made to the analysis of protein hydrolysates and to the sensitivity of the ninhydrin reaction.
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Über die Trennung von Aminosäuren mit Hilfe der Dünnschichtchromatographie
E. Mutschler,H. Rochelmeyer +1 more
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Séparation rapide des acides aminés par chromatographie ascendante bidimensionnelle sur papier. (Etudes chromatographiques I)
TL;DR: In this paper, it is possible to separer quantitativement in deux chromatographies bidimensionnelles ascendantes les 18 acides amines courants in moins de 13 heures.