The rigid layer of the cell wall of Escherichia coli strain B.
W. Weidel,H. Frank,H. H. Martin +2 more
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The structure conferring rigidity and shape on the complex cell wall of Escherichia coli strain B has been isolated in a state virtually free from other wall material and shows a characteristic surface pattern which indicates the fairly simple principles of its construction.Abstract:
SUMMARY: The structure conferring rigidity and shape on the complex cell wall of Escherichia coli strain B has been isolated in a state virtually free from other wall material. It shows a characteristic surface pattern which, together with observations on its mode of disintegration by phage enzyme or lysozyme, indicates the fairly simple principles of its construction.read more
Citations
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Journal ArticleDOI
Characterization of the Major Envelope Protein from Escherichia coli REGULAR ARRANGEMENT ON THE PEPTIDOGLYCAN AND UNUSUAL DODECYL SULFATE BINDING
TL;DR: The major envelope protein from Escherichia coli has been purified by differential heat extraction in dodecyl sulfate and subsequently freed of the detergent, and its molecular weight agrees with that derived from the mobility of the major band observed in standard dodecYL sulfate gel electrophoretic analysis of unfractionated cell envelopes after treatment at 100°.
Book ChapterDOI
5 Analysis of the Chemical Composition and Primary Structure of Murein
TL;DR: This chapter discusses the analysis of the chemical composition and primary structure of the murein, the main cell wall polymer of eubacteria and is common to both Gram-negative and Gram-positive bacteria.
Journal ArticleDOI
Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure.
Volkmar Braun,Kurt Rehn +1 more
TL;DR: It seems that cleavage of only one peptide bond adjacent to the lysine link between the lipoprotein and the murein causes the rapid decrease of the absorbance and as shown by electron microscopic exmination of ultrathin sections of trypsin treated cell walls, two separated membrane structures appear which otherwise are closely adjacent to one another.
Journal ArticleDOI
Bacterial cell shape
TL;DR: How a bacterium gains and maintains its shape, the challenges still confronting us and emerging strategies for answering difficult questions in this rapidly evolving field are discussed.
References
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Journal ArticleDOI
Bacterial Protoplasts Induced by Penicillin
TL;DR: 3 S. Cohen and R. Levi-Montalcini, Cancer Research (in press).
Journal ArticleDOI
Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls.
M.R.J. Salton,R.W. Horne +1 more
TL;DR: The preparation of pure cell walls has been followed by examination of material in the electron microscope and ultra-violet absorption spectra show no maximum corresponding to nucleic acid or purine-pyrimidine compounds.
Journal ArticleDOI
Uridine-5'-pyrophosphate derivatives. II. Isolation from Staphylococcus aureus
Journal ArticleDOI
Electron microscopical studies of phage multiplication: I. A method for quantitative analysis of particle suspensions
E. Kellenberger,Werner Arber +1 more
TL;DR: The agar-filtration method for preparing particle suspensions for the electron microscope is described and its application to particle counting is studied and the coefficient of variation is found to be about 15%.
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