The search for molecular mimicry in proteins carried by extracellular vesicles secreted by cells infected with Plasmodium falciparum.
TLDR
In this paper, a set of eight proteins with a remarkable resemblance to human proteins were found to be moonlighting proteins carried by Plasmodium falciparum molecules carried by extracellular vesicles.Abstract:
Red blood cells infected with Plasmodium falciparum secrete extracellular vesicles in order to facilitate the survival and infection of human cells. Various researchers have studied the composition of these extracellular vesicles and identified the proteins contained inside. In this work, we used that information to detect potential P. falciparum molecules that could be imitating host proteins. We carried out several searches to detect sequences and structural similarities between the parasite and host. Additionally, the possibility of functional mimicry was explored in line with the potential role that each candidate can perform for the parasite inside the host. Lastly, we determined a set of eight sequences (mainly moonlighting proteins) with a remarkable resemblance to human proteins. Due to the resemblance observed, this study proposes the possibility that certain P. falciparum molecules carried by extracellular vesicles could be imitating human proteins to manipulate the host cell's physiology.read more
Citations
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Sialylated N ‐glycans mediate monocyte uptake of extracellular vesicles secreted from Plasmodium falciparum ‐infected red blood cells
Hila Ben Ami Pilo,Sana Khan Khilji,Jost Lühle,Karina Biskup,Bar Levy Gal,Irit Rosenhek Goldian,Daniel Alfandari,Or-Yam Revach,E. Kiper,Mattia I. Morandi,Ron Rotkopf,Ziv Porat,Véronique Blanchard,Peter H. Seeberger,Neta Regev-Rudzki,Oren Moscovitz +15 more
TL;DR: In this paper , the authors show that Plasmodium falciparum (Pf)-infected red blood cells (RBCs) carry significantly higher sialylated complex N-glycans than those derived from healthy RBCs.
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Proteome-wide comparison of tertiary protein structures reveals molecular mimicry in Plasmodium-human interactions
Viraj Muthye,James D. Wasmuth +1 more
TL;DR: In this paper , the authors presented the first proteome-level search for tertiary structure similarity between proteins from Plasmodium falciparum, a malaria-causing parasite, and humans.
Posted ContentDOI
Proteome-wide comparison of tertiary protein structures reveal extensive molecular mimicry in Plasmodium-human interactions
Viraj Muthye,James D. Wasmuth +1 more
TL;DR: In this paper , the first proteome-level search for tertiary structure similarity between the proteins from Plasmodium falciparum and human was performed, and it was shown that seven evolved to molecularly mimic a human protein.
References
More filters
Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
The Protein Data Bank
Helen M. Berman,John D. Westbrook,Zukang Feng,Gary L. Gilliland,Talapady N. Bhat,Helge Weissig,Ilya N. Shindyalov,Philip E. Bourne +7 more
TL;DR: The goals of the PDB are described, the systems in place for data deposition and access, how to obtain further information and plans for the future development of the resource are described.
Journal ArticleDOI
MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability
Kazutaka Katoh,Daron M. Standley +1 more
TL;DR: This version of MAFFT has several new features, including options for adding unaligned sequences into an existing alignment, adjustment of direction in nucleotide alignment, constrained alignment and parallel processing, which were implemented after the previous major update.
Journal ArticleDOI
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
TL;DR: The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine and has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses.
Journal ArticleDOI
The Phyre2 web portal for protein modeling, prediction and analysis
Lawrence A. Kelley,Stefans Mezulis,Christopher M. Yates,Christopher M. Yates,Mark N. Wass,Mark N. Wass,Michael J.E. Sternberg +6 more
TL;DR: An updated protocol for Phyre2, which uses advanced remote homology detection methods to build 3D models, predict ligand binding sites and analyze the effect of amino acid variants for a user's protein sequence.