The Somatic Function of the Germ Nucleus in Pseudourostyla cris tat a: Asexual Reproduction and Stomatogenesis

TLDR: The micronuclei of the ciliated protozoan Pseudourostyla cristata were eliminated by amputation shortly before binary fusion and the amicronucleate cell lines derived from regenerants were maintained for more than a year, displaying a lower viability and reduced vigor in asexual propagation.

Abstract: The micronuclei of the ciliated protozoan Pseudourostyla cristata were eliminated by amputation shortly before binary fusion. The amicronucleate cell lines derived from regenerants were maintained for more than a year. They exhibited a lower viability and reduced vigor in asexual propagation. There was some improvement in the growth of the cell lines 1 mo after operation, but the growth rate remained subnormal even up to 1 yr of culture. The exact cause of the poor growth and survival in the first 3 wk after operation, whether the loss of the micronucleus or operational damage, remains to be determined. It is nevertheless clear that the micronucleus is important for subsequent asexual propagation. The amicronucleate cell lines were permanently crippled in morphogenesis, unlike the situation in Paramecium amicronucleates in which stomatogenesis returned to near-normal during asexual propagation. They always included some cells with a characteristically defective adoral zone of membranelles, reduced number of frontal-ventral-transverse cirri, and reduced body length. They were also reluctant to encyst. It is evident that the micronucleus is important for maintaining normality of the oral apparatus. It is postulated that the permanent stomatogenic crippling of amicronucleates might be related to genomic reduction in the developing macronucleus in sexual reproduction, as exhibited by other hypotrichs. The morphological defects associated with the adoral zone of membranelles may be rationalized as arising from the spreading of a zone of degeneration in the cortex affecting the left edge of the membranelles.