scispace - formally typeset
Open AccessJournal ArticleDOI

The Specific Binding of Biebrich Scarlet to the Active Site of α-Chymotrypsin

Alexander N. Glazer
- 10 Oct 1967 - 
- Vol. 242, Iss: 19, pp 4528-4533
Reads0
Chats0
TLDR
In this article, the binding site for the dye Biebrich Scarlet overlaps the active site region in α-chymotrypsin, and showed no significant spectral perturbation in the presence of these proteins, under the conditions used in this study.
About: 
This article is published in Journal of Biological Chemistry.The article was published on 1967-10-10 and is currently open access. It has received 32 citations till now. The article focuses on the topics: Biebrich scarlet & Active site.

read more

Citations
More filters
Journal ArticleDOI

Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships.

Emil L. Smith, +4 more
- 10 May 1968 - 
TL;DR: Within the subtilisins, there are a number of distinct repetitions of sequence; this suggests that the proteins may have evolved from shorter peptide chains by some process of extension of the sequence.
Book ChapterDOI

16 Subtilisins: Primary Structure, Chemical and Physical Properties

Francis S. Markland, +1 more
- 01 Jan 1971 - 
TL;DR: The sequence of subtilisin BPN' was deduced from studies of the tryptic, chymotryptic, peptic, and cyanogen bromide digests, and there is no apparent homology with the sequences of the pancreatic proteinases.
Journal ArticleDOI

Interaction of Cibacron Blue 3G-A and related dyes with nucleotide-requiring enzymes.

Robert S. Beissner, +1 more
- 01 Jul 1978 - 
TL;DR: Inhibition studies indicate that the portion of the dye molecule necessary for effective inhibition of nucleotide binding is a structure similar to 1-amino-4(4′-aminophenylamino)-anthraquinone-2,3′-disulfonic acid (ASSO; III).
Journal ArticleDOI

Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646.

Tao Li, +1 more
- 01 Jun 1997 - 
TL;DR: An aryl aldehyde oxidoreductase from Nocardia sp.
Book ChapterDOI

Conformational adaptability in enzymes.

Nathan Citri
- 01 Jan 1973 - 
References
More filters
Journal ArticleDOI

Measurement of protein-binding phenomena by gel filtration.

J.P. Hummel, +1 more
- 08 Oct 1962 - 
Journal ArticleDOI

Sulfonyl Fluorides as Inhibitors of Esterases. I. Rates of Reaction with Acetylcholinesterase, α-Chymotrypsin, and Trypsin

David Fahrney, +1 more
- 01 Apr 1963 - 
Journal ArticleDOI

The spectrophotometric determination of the operational normality of an alpha-chymotrypsin solution.

Gregory R. Schonbaum, +2 more
- 01 Nov 1961 - 
Journal ArticleDOI

Peptide-protein interaction as studied by gel filtration.

Gordon F. Fairclough, +1 more
- 01 Feb 1966 - 
Journal ArticleDOI

Fluorescent Probes for Conformational States of Proteins. II. The Binding of 2-p-Toluidinylnaphthalene-6-sulfonate to α-Chymotrypsin*

William O. McClure, +1 more
- 01 Feb 1967 - 
Related Papers (5)

Specific binding of thionine to the active site of trypsin.

Alexander N. Glazer
- 25 Jul 1967 - 

On the interaction of the active site of α-chymotrypsin with chromophores: Proflavin binding and enzyme conformation during catalysis

Sidney A. Bernhard, +2 more
- 01 Jul 1966 - 

On the Prevalence of „Nonspecific” Binding at the Specific Binding Sites of Globular Proteins

A. N. Glazer
- 01 Apr 1970 - 

The time-dependent specific interation of 4-(4'-aminophenylazo)phenylarsonic acid with subtilsins.

A N Glazer
- 01 Mar 1968 - 

The interaction of yeast hexokinase with Procion Green H-4G

Yannis D. Clonis, +2 more
- 01 Jul 1981 -