Specific binding of thionine to the active site of trypsin.
Reads0
Chats0
TLDR
Thionine to chymotrypsin is not affected by substrates, inhibitors, or chemically reactive substrate analogues; hence, the thionine-binding site in chymotsin does not appear to include any part of the active site region.About:
This article is published in Journal of Biological Chemistry.The article was published on 1967-07-25 and is currently open access. It has received 32 citations till now. The article focuses on the topics: Thionine & Chymotrypsinogen.read more
Citations
More filters
Journal ArticleDOI
Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships.
TL;DR: Within the subtilisins, there are a number of distinct repetitions of sequence; this suggests that the proteins may have evolved from shorter peptide chains by some process of extension of the sequence.
Book ChapterDOI
16 Subtilisins: Primary Structure, Chemical and Physical Properties
TL;DR: The sequence of subtilisin BPN' was deduced from studies of the tryptic, chymotryptic, peptic, and cyanogen bromide digests, and there is no apparent homology with the sequences of the pancreatic proteinases.
Journal ArticleDOI
Induction of the bovine trypsinogen-trypsin transition by peptides sequentially similar to the N-terminus of trypsin.
Wolfram Bode,Robert Huber +1 more
TL;DR: Crystallographic structural studies of the trypsin-trypsinogen-PTI system found indeed that the complex between PTI and trypsInogen crystallizes isomorphously to the tryPSinPTI complex and is structurally extremely similar.
Journal ArticleDOI
Interaction of Cibacron Blue 3G-A and related dyes with nucleotide-requiring enzymes.
TL;DR: Inhibition studies indicate that the portion of the dye molecule necessary for effective inhibition of nucleotide binding is a structure similar to 1-amino-4(4′-aminophenylamino)-anthraquinone-2,3′-disulfonic acid (ASSO; III).
Journal ArticleDOI
Fluorescence and structure of proteins as measured by incorporation of fluorophore. IV. Synthesis and fluorescence characteristics of N-(p-(2-benzimidazolyl)phenyl) maleimide.
TL;DR: In this paper, a sulfhydryl reagent N-(p-(2- benzimidazolyl)phenyl ) maleimide (BIPM) was synthesized and the fluorescence characteristics of its derivatives were studied.
References
More filters
Journal ArticleDOI
Evidence for an Active-Center Histidine in Trypsin through Use of a Specific Reagent, 1-Chloro-3-tosylamido-7-amino-2-heptanone, the Chloromethyl Ketone Derived from Nα-Tosyl-L-lysine*
Journal ArticleDOI