Journal ArticleDOI
Trace metal levels in commercially prepared tissue culture media
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TLDR
Variability in establishing primary cultures of corneal endothelial cells was traced to high lead levels in commercially prepared tissue culture media, and a strong case is made for continued diligent efforts to expand analytical horizons and the definition of substances in culture media.Abstract:
Four trace elements, lead, copper, tin and zinc, in addition to certain electrolytes, were measured in 11 commercially prepared tissue culture media. Glass media bottles and plastic tissue culture dishes and flasks were treated with a HCl acid solution to determine the amounts of trace metals leached from their surfaces. Zinc, lead and copper were detected in all media. Tin was detected only in RPMI Medium 1640, fetal bovine serum, minimum essential medium and penicillin-streptomycin. It is possible that a major cause of variability in tissue culture experimental results may be due to effects on growth caused by fluctuation in trace element contamination from batch to batch. Variability in establishing primary cultures of corneal endothelial cells was traced to high lead levels in commercially prepared tissue culture media. A strong case is made for continued diligent efforts to expand analytical horizons and our definition of substances in culture media.read more
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Effects of copper on CHO cells: cellular requirements and product quality considerations.
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References
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Journal ArticleDOI
A micro-sampling method for the rapid determination of lead in blood by atomic-absorption spectrophotometry
TL;DR: Thirty-nine blood samples with lead concentrations ranging from 19 to 245 µg per 100 ml were analysed by the method described and by automated colorimetry involving the use of dithizone, and the correlation coefficient between the results was 0·989.
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Journal ArticleDOI
Clonal growth of Chinese hamster cell lines in protein-free media
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Journal ArticleDOI
Growth effects of tin compounds in rats maintained in a trace element-controlled environment
Klaus Schwarz,Klaus Schwarz,David B. Milne,David B. Milne,Elizabeth Vinuyard,Elizabeth Vinuyard +5 more
TL;DR: In rats maintained on purified amino acid diets in trace element-controlled isolators, supplements of tin caused a significant growth effect, suggesting that tin is a hitherto unrecognized essential trace element.