Journal ArticleDOI
Trichoderma harzianum IOC-4038: A Promising Strain for the Production of a Cellulolytic Complex with Significant β-Glucosidase Activity from Sugarcane Bagasse Cellulignin
Aline Machado de Castro,Kelly Cristina Nascimento Rodrigues Pedro,Juliana Cunha da Cruz,Marcela Costa Ferreira,Selma Gomes Ferreira Leite,Nei Pereira +5 more
TLDR
The ability of a fungal strain, Trichoderma harzianum IOC-4038, to produce cellulases on a novel material, xylan free and cellulose rich, generated from sugarcane bagasse, named partially delignified cellulignin is reported.Abstract:
Sugarcane bagasse is an agroindustrial residue generated in large amounts in Brazil. This biomass can be used for the production of cellulases, aiming at their use in second-generation processes for bioethanol production. Therefore, this work reports the ability of a fungal strain, Trichoderma harzianum IOC-4038, to produce cellulases on a novel material, xylan free and cellulose rich, generated from sugarcane bagasse, named partially delignified cellulignin. The extract produced by T. harzianum under submerged conditions reached 745, 97, and 559 U L−1 of β-glucosidase, FPase, and endoglucanase activities, respectively. The partial characterization of this enzyme complex indicated, using a dual analysis, that the optimal pH values for the biocatalysis ranged from 4.9 to 5.2 and optimal temperatures were between 47 and 54 °C, depending on the activity studied. Thermal stability analyses revealed no significant decrease in activity at 37 °C during 23 h of incubation. When compared to model strains, Aspergillus niger ATCC-16404 and Trichoderma reesei RutC30, T. harzianum fermentation was faster and its extract showed a better balanced enzyme complex, with adequate characteristics for its application in simultaneous saccharification and fermentation processes.read more
Citations
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Journal ArticleDOI
Green synthesis of silver nanoparticles by Trichoderma harzianum and their bio-efficacy evaluation against Staphylococcus aureus and Klebsiella pneumonia
TL;DR: In this paper, a simple green and eco-friendly route using cell filtrate was used to synthesize silver nanoparticles from the fungus Trichoderma harzianum.
Journal ArticleDOI
Sequential solid-state and submerged cultivation of Aspergillus niger on sugarcane bagasse for the production of cellulase.
F. M. Cunha,Mateus N. Esperança,Teresa Cristina Zangirolami,Alberto C. Badino,Cristiane S. Farinas +4 more
TL;DR: An endoglucanase productivity of 57 ± 13 IU/L/h was achieved in bubble column cultivations prepared using the new method, representing an approximately 3-fold improvement compared to conventional submerged fermentation.
Journal ArticleDOI
Production of cellulolytic enzymes from ascomycetes: comparison of solid state and submerged fermentation
Gustav Hammerich Hansen,Mette Lübeck,Jens Christian Frisvad,Peter Stephensen Lübeck,Birgitte Andersen +4 more
TL;DR: In this paper, the authors provide an overview of enzymatic profiles for several ascomycetes covering phylogenetically distinct genera and species, and provide guidelines for directly comparing cellulolytic enzyme production in SSF with SmF to advance future research of enzyme production.
Journal ArticleDOI
Antimicrobial activity and green synthesis of silver nanoparticles using Trichoderma viride
Abdallah M. Elgorban,A N Al-Rahmah,Shaban R.M. Sayed,Abdurahman H. Hirad,Ashraf Abdеl-Fattah Mostafa,Ali H. Bahkali +5 more
TL;DR: In this article, the fungus Trichoderma viride was used for the synthesis of biogenic silver nanoparticles, which significantly inhibited the growth of all tested pathogenic bacteria.
Journal ArticleDOI
An overview on the recent developments in fungal cellulase production and their industrial applications
TL;DR: The strategies for cost reduction and hyper cellulase production like mixed culture and genetic manipulation are discussed along with global players of cellulase producers/suppliers.
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