Two-step purification of tag-free norovirus-like particles from silkworm larvae (Bombyx mori).
Jirayu Boonyakida,LIDIANE PEREIRA DE ALBUQUERQUE,Doddy Irawan Setyo Utomo,Fahmida Nasrin Soma,Enoch Y. Park +4 more
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TLDR
In this paper, a silkworm-derived norovirus-like particle (NoV-LPs) was extracted from fat body lysate by density gradient centrifugation and further purified using immobilized metal affinity chromatography.About:
This article is published in Protein Expression and Purification.The article was published on 2022-02-01 and is currently open access. It has received 3 citations till now. The article focuses on the topics: Bombyx mori & Norovirus.read more
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Virus-like Particles as Antiviral Vaccine: Mechanism, Design, and Application
TL;DR: In this article , the authors discuss the mechanism of VLPs vaccine inducing cellular and humoral immune responses and outline the impact of size, shape, surface charge, antigen presentation, genetic and chemical modification, and expression systems when constructing effective VLI-based vaccines.
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Improvement of Modular Protein Display Efficiency in SpyTag-Implemented Norovirus-like Particles.
TL;DR: In this paper , a modular display platform for protein decoration on norovirus-like particles (NoV-LPs) was developed by combining the NoV-LP scaffold with the SpyTag/SpyCatcher bioconjugation system.
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Simple, versatile, and practical impedimetric immunosensor based on gold nanoparticle-polyaniline nanocomposite for clinical dengue virus detection
TL;DR: In this paper , a simple, versatile, and practical impedimetric immunosensor for virus detection was constructed by electrostatically bound gold nanoparticles to polyaniline (PAni) and subsequently deposited on the surface of the PAni-coated gold electrode.
References
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Baculovirus as versatile vectors for protein expression in insect and mammalian cells
TL;DR: Novel vector design and cell engineering approaches will serve to further enhance the value of baculovirus technology.
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Monoclonal versus polyclonal antibodies: distinguishing characteristics, applications, and information resources.
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Perspectives of immobilized-metal affinity chromatography.
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Updated classification of norovirus genogroups and genotypes
Preeti Chhabra,Miranda de Graaf,Gabriel I. Parra,Martin C.W. Chan,Kim Y. Green,Vito Martella,Qiuhong Wang,Peter A. White,Kazuhiko Katayama,Harry Vennema,Marion Koopmans,Jan Vinjé +11 more
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Tables for estimating sedimentation through linear concentration gradients of sucrose solution
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