Journal ArticleDOI
Ultrasound lethality to synchronous and asynchronous chinese hamster V-79 cells
TLDR
The M and S phases of the cell cycle were more resistant with respect to cell lysis and loss of reproductive integrity than were the G1 and G2 phases.Abstract:
Chinese hamster V-79 cells were exposed in suspensions for 1–15 min to 1.1 MHz continuous wave (CW) ultrasound at axial intensifies from 0.25–30 W/cm2. Cell lysis was evidenced by a decrease in the number of intact cells, and loss of reproductive integrity was evidenced by a decrease in the plating efficiency of the remaining intact cells. The magnitude of these effects was a function of both intensity and exposure duration. Mitotically synchronized cells displayed a differential sensitivity depending upon cell cycle position. The M and S phases of the cell cycle were more resistant with respect to cell lysis and loss of reproductive integrity than were the G1 and G2 phases.read more
Citations
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A review of in vitro bioeffects of inertial ultrasonic cavitation from a mechanistic perspective
TL;DR: This selective review of the biological effects of ultrasound presents a synopsis of the current understanding of how cells insonated in vitro are affected by inertial cavitation from the standpoint of physical and chemical mechanisms.
Journal ArticleDOI
Evidence for free radical production by ultrasonic cavitation in biological media
TL;DR: It is demonstrated the formation of the highly reactive .OH and .H radicals in the insonated media, even in the presence of natural radical scavengers in the mammalian derived products.
Journal ArticleDOI
Biological effects of shock waves: cell disruption, viability, and proliferation of L1210 cells exposed to shock waves in vitro.
TL;DR: Overall proliferation of cells which were trypan blue negative after exposure exceeded 70% of the proliferation of sham treated controls, except after 1000 shocks at 25 kV, where proliferation was reduced to 42%.
Journal ArticleDOI
Ultrasonically induced alterations of cultured tumour cells
TL;DR: It was demonstrated that cells were most sensitive when undergoing M- and S-phases of the cell cycle, and ultrasonically induced changes of the cytoskeleton seem to be non-specific and temporary.
References
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Journal ArticleDOI
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
TL;DR: Application of the methods described in this paper as a tool for quantitative study of normal mammalian cell growth, physiology, genetics, and biochemistry, and the response of cells to drugs, viruses, high energy radiation, and other agents have been indicated.
Journal ArticleDOI
Variations in Several Responses of HeLa Cells to X-Irradiation during the Division Cycle
Toyozo Terasima,L. J. Tolmach +1 more
TL;DR: Several responses of synchronized populations of HeLa S3 cells were measured after irradiation with 220 kev x-rays at selected times during the division cycle, showing survival is maximal when cells are irradiated in the early post-mitotic and the pre-mitosis phases of the cycle, and minimal in the mitotic and late G(1) or early DNA synthetic (S) phases.
X-Ray Sensitivity during the Cell Generation Cycle of
W. K. Sinclair,R. A. Morton +1 more
TL;DR: To determine X-ray sensitivity more accurately at all stages, lethal amounts of tritiated thymidine (HTdR) are used to inactivate the S cells and since the previous technique could not be used to obtain the sensitivity of mitotic (M) cells, the procedure is modified so that this may be accomplished.
Journal ArticleDOI
X-ray sensitivity during the cell generation cycle of cultured Chinese hamster cells.
Warren K. Sinclair,R. A. Morton +1 more
TL;DR: In this paper, the authors used tritiated thymidine (HTdR) to inactivate the X-ray sensitivity of Chinese hamster cells to determine whether cells in S had a constant or variable sensitivity throughout the synthetic period.
Journal ArticleDOI
Cyclic x ray responses in mammalian cells in vitro.
TL;DR: Survival data obtained so far show that, in cells with a short G1, cells are most sensitive in mitosis and in G2, less sensitive in G 1, and least sensitive during the latter part of the S period, which can be explained in terms of a two-component model of division delay.