Journal ArticleDOI
Ultraviolet Light-Induced Pathology in the Eye: Associated Changes in Ocular Aldehyde Dehydrogenase and Alcohol Dehydrogenase Activities
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TLDR
The data support earlier proposals for major roles for these corneal enzymes in assisting the cornea in protecting the eye against UVR-induced tissue damage.Abstract:
Adult male C57BL/6J inbred mice were subjected to ultraviolet radiation (UVR) exposure (302-nm peak wavelength; average intensity 282 microW/cm2) for 1 h and monitored for ocular aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH) activity changes over a period of 25 days. Dramatic reductions in activities were observed by 4-6 days postexposure, resulting in enzyme levels of 15-16% of control animals. Major decreases in corneal enzyme levels were predominantly responsible for these changes. Ocular morphology was observed throughout using a photoslit-lamp biomicroscope, with maximum corneal clouding occurring at days 4-6. These data support earlier proposals for major roles for these corneal enzymes in assisting the cornea in protecting the eye against UVR-induced tissue damage.read more
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Journal ArticleDOI
Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily.
TL;DR: What is currently known about each member of the human ALDH superfamily, composed of NAD(P)+-dependent enzymes that catalyze aldehyde oxidation, is presented including the pathophysiological significance of these enzymes.
Journal ArticleDOI
Evidence of Oxidative Stress in Human Corneal Diseases
Rajeev Buddi,Brian Lin,Shari R. Atilano,Nadia C. Zorapapel,M. Cristina Kenney,Donald J. Brown +5 more
TL;DR: The results suggest that keratoconus corneas do not process reactive oxygen species in a normal manner, which may play a major role in the pathogenesis of this disease.
Journal ArticleDOI
Ultraviolet Radiation-Induced Corneal Degeneration in 129 Mice:
Kimberly M. Newkirk,Heather L. Chandler,Allison E. Parent,Donn C. Young,Carmen M. H. Colitz,David A. Wilkie,Donna F. Kusewitt +6 more
TL;DR: It is proposed that the loss of keratocytes observed in the chronic study was due to UVR-induced apoptosis, and this is the first report of the occurrence of spontaneous cataracts in 129 mice.
Journal ArticleDOI
Aldehyde dehydrogenases and cell proliferation
TL;DR: High ALDH3A1 expression and activity have been correlated with cell proliferation, resistance against aldehydes derived from lipid peroxidation, and resistance against drug toxicity, such as oxazaphosphorines.
Journal ArticleDOI
Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea
TL;DR: The metabolic function of ALDH3A1 is supported as a part of a corneal cellular defence mechanism against oxidative damage caused by aldehydic products of lipid peroxidation.