Journal ArticleDOI
Uranium speciation in biological medium by means of capillary electrophoresis and time-resolved laser-induced fluorescence
TLDR
In this article, the use of capillary electrophoresis (CE) and time-resolved laser-induced fluorescence (TRLIF) is used to characterize the different complexes of uranium.Abstract:
The study of the chemical behavior of uranium in biological medium is still of great interest. By the use of capillary electrophoresis (CE) and time-resolved laser-induced fluorescence (TRLIF), it is possible to characterize the different complexes of uranium. Hence, CE, using the isoelectric focusing mode (CIEF), allows for the separation of the different complexes as a function of their isoelectric points (pI) and TRLIF as a speciation method leads to the identification at very low level of different uranyl complexes by temporal resolution and spectral deconvolution. Results obtained on various inorganic chemical systems (phosphate, bicarbonate) together with biological systems (citrate, transferrin) will be presented and discussed. The complexation between uranium and human transferrin has been pointed out through CIEF.read more
Citations
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Journal ArticleDOI
Characterization and remediation of soils contaminated with uranium.
TL;DR: The objective of this paper is to develop a better understanding of the techniques for the remediation of soils polluted with radionuclides (uranium in particular), considering the chemical forms of uranium, including depleted uranium (DU) in soil and other environmental media, their characteristics and concentrations.
Journal ArticleDOI
Uranium(VI) bio-coordination chemistry from biochemical, solution and protein structural data
J. David Van Horn,Huan Huang +1 more
TL;DR: The biocoordination chemistry of the uranyl cation (uranium(VI), [UO2]2+) is assessed utilizing data from the Protein Databank, solution thermodynamic data and biochemical experiments.
Journal ArticleDOI
Advances in technologies for the measurement of uranium in diverse matrices
TL;DR: An overview of the advances in technologies, which can be used in the field as well as in a laboratory for the measurement of uranium in diverse matrices like, waters, minerals, mineralized rocks, and other beneficiation products for its exploration and processing industries is presented.
Journal ArticleDOI
Uptake and release of metal ions by transferrin and interaction with receptor 1
TL;DR: A competition with iron is possible and implies that metal internalization is more related to kinetics than thermodynamics, which implies its possible liberation in the cell.
Journal ArticleDOI
Interaction of uranium(VI) with lipopolysaccharide.
TL;DR: Investigating the interaction of the uranyl cation with lipopolysaccharide from Pseudomonas aeruginosa by using potentiometric titration and time-resolved laser-induced fluorescence spectroscopy over a wide pH and concentration range shows that at an excess of LPS uranyl phosphoryl coordination dominates, whereas at a slight deficit on LPS compared to uranyl, carboxyl groups also become important for uranyl coordination.
References
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Journal ArticleDOI
High-performance electrophoresis : Elimination of electroendosmosis and solute adsorption
TL;DR: In this article, it was shown that electroendosmosis and adsorption of solutes onto the inside of the electrophoresis chamber should not occur in free high-performance electrophorensis when the narrow-bore tube is coated with a mono-molecular layer of polyacrylamide.
Book
Chemical thermodynamics of uranium
TL;DR: The NEA-TDB data base system as discussed by the authors is used for the extraction of the NEA TDB data set and its associated auxiliary data sets, such as ammonium, oxide, hydride and hydroxide species.
Book ChapterDOI
Structure and Reactivity of Transferrins
TL;DR: This chapter provides an overview of structure and reactivity of transferrins, a family of metal-binding proteins having significant role in biological research.
Journal ArticleDOI
Fast and high resolution analysis of human serum transferrin by high performance isoelectric focusing in capillaries.
Ferenc Kilár,Stellan Hjertén +1 more
TL;DR: The new high performance capillary technique was employed for isoelectric focusing to resolve the many components in transferrin preparations, and observed that chelating agents may release iron from micro‐syringes having metal pistons causing the formation of iron‐transferrin complexes.
Journal ArticleDOI
Uranium speciation in solution by time-resolved laser-induced fluorescence
Abstract: Complexation studies of radionuclides (such as uranium) are important to perform in order to predict their migration behavior in natural systems. Time-resolved laser-induced fluorescence, which is a very selective and sensitive method for actinide and lanthanide analysis, is used to study the interactions between uranyl cation and hydroxide ions at low uranium concentration (0.4 [mu]M). The principle of the study is based on time resolution and spectral convolution since free uranyl ion (UO[sub 2][sup 2+]) and first hydroxide complex give specific fluorescence spectra characterized by their spectral shift and fluorescence lifetime. Spectroscopic data (main fluorescence wavelengths, half-bandwidth, lifetime) for the first hydrolyzed complex UO[sub 2]OH[sup +] are obtained. From experimental spectra and time-resolved spectral convolution, the proportion of each species (free uranyl, complexed uranyl) can be estimated at different pH. 33 refs., 5 figs., 3 tabs.