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Open AccessJournal ArticleDOI

Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.

M P Roiz, +3 more
- 01 May 1995 - 
- Vol. 33, Iss: 5, pp 1389-1391
TLDR
Restriction fragment length polymorphism was used to study 75 clinical isolates identified as Mycobacterium avium and two repetitive insertion sequences, IS1311 and IS900, exhibited marked polymorphism with both probes.
Abstract
Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes.

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Citations
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Journal ArticleDOI

Epidemiology of Infection by Nontuberculous Mycobacteria

TL;DR: No specific nutrient(s) occurred in the waters of the southeastern United States that could account for a greater frequency of isolation of MaIS organisms from that region, and temperature is apparently a factor influencing the number of MAIS organisms in waters.
Journal ArticleDOI

Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine.

TL;DR: The epidemiology, pathogenesis, diagnostics, and disease control measures of this important veterinary pathogen are reviewed and proposals for the applications of animal models and recombinant technology to the development of diagnostic, control, and therapeutic measures are concluded.
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Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements.

TL;DR: The state of the art of the main molecular typing methods for Mycobacterium tuberculosis complex and non-M.
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Mycobacteria distenct from Mycobacterium avium subsp. paratuberculosis isolated from the faeces of ruminants possess IS900-like sequences detectable IS900 polymerase chain reaction: implications for diagnosis.

TL;DR: It is recommended that the adoption of restriction endonuclease analysis of IS 900 PCR product is adopted as a routine precaution to prevent the reporting of false positiveIS 900 PCR results.
References
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Journal ArticleDOI

Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.

TL;DR: In this article, the authors proposed a standardized technique which exploits variability in both the number and genomic position of IS6110 to generate strain-specific patterns for DNA fingerprinting of Mycobacterium tuberculosis.
Journal ArticleDOI

Mycobacterium avium complex infection in the acquired immunodeficiency syndrome.

TL;DR: Before the acquired immunodeficiency syndrome (AIDS) epidemic, disseminated infection with M. avium complex was extremely rare; by 1980, only 24 cases had been reported in the medical literature; beginning in 1982, however, the number of cases increased dramatically.
Journal ArticleDOI

Transmission of tuberculosis in New York City. An analysis by DNA fingerprinting and conventional epidemiologic methods

TL;DR: In the inner-city community the authors studied, recently transmitted tuberculosis accounts for approximately 40 percent of the incident cases and almost two thirds of drug-resistant cases.
Journal ArticleDOI

Sequence and characteristics or IS900, an insertion element identified in a human Crohn's disease isolate or Mycobacterium paratuberculosis

TL;DR: It is proposed that IS900, IS110, and similar insertion elements recently identified in disease isolates of Mycobacterium avium are members of a phylogenetically related family.
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