Journal ArticleDOI
Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis.
Andrea Peano,Annarita Molinar Min,M. Beccati,Arianna Menzano,Mario Pasquetti,Maria Grazia Gallo +5 more
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TLDR
The proteins visualised in this study may represent diagnostic markers of dermatophyte infection and should be further evaluated for their role in the cellular immune response of dogs with dermatophytosis.Abstract:
Summary
Western blotting was used to describe the Microsporum canis proteins with antigenic activity in dogs with dermatophytosis. Electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. The proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 M. canis, 3 M. gypseum, 1 Trichophyton mentagrophytes; group A), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no clinical cutaneous signs (group B, n = 42). Nine principal IgG-binding proteins with apparent molecular weights of 180, 144, 130, 120, 102, 96, 80, 68, and 48 kD were visualised on group A blots. For these proteins, serological cross-reactivity with different strains of M. canis may be indirectly confirmed, whereas additional proteins were found to react with sera from individual dogs. The proteins visualised in this study may represent diagnostic markers of dermatophyte infection. The proteins should be further evaluated for their role in the cellular immune response of dogs with dermatophytosis.read more
Citations
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Journal ArticleDOI
Development of enzyme immunoassays (ELISA and Western blot) for the serological diagnosis of dermatophytosis in symptomatic and asymptomatic cats.
A. E Santana,Carlos Pelleschi Taborda,J. S Severo,Glauce Mary Gomes Rittner,Julián E. Muñoz,Carlos Eduardo Larsson +5 more
TL;DR: The study concluded that ELISA and WB were useful tools to reliably detect cats that have been exposed to M. canis.
Book ChapterDOI
Cutaneous, Subcutaneous and Systemic Mycology
TL;DR: The fungal aetiology of tinea was first detected by Robert Remak, a Polish physician who first observed the presence of hyphae in the crusts of favus, a landmark in medical history because this is the first description of a microbe causing a human disease.
Journal ArticleDOI
Dermatopathology – the link between ancillary techniques and clinical lesions
TL;DR: The utility, strengths, advantages/disadvantages and challenges associated with each technique are discussed and dermatopathology will remain the important link between bench top science, results from ancillary techniques available and clinical veterinary dermatology.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Allergy and Dermatophytes
TL;DR: Characterizing the immunologic properties of Trichophyton allergens and defining immune mechanisms which drive dichotomous responses are pivotal to understanding the dermatophyte-allergy relationship.
Journal ArticleDOI
The epidemiology of canine and feline dermatophytoses in southern Italy.
Claudia Cafarchia,D. Romito,Mariateresa Sasanelli,Riccardo Paolo Lia,Gioia Capelli,Domenico Otranto +5 more
TL;DR: Young dogs and cats, especially those younger than 1 year, showed a statistically significant higher prevalence of M. canis infection than older animals, while male dogs were more affected by dermatophytes.
Journal ArticleDOI
Dermatophytes isolated from symptomatic dogs and cats in Tuscany, Italy during a 15-year-period.
TL;DR: The annual distribution of the infections in dogs showed a significantly higher incidence for M. gypseum in summer versus winter and spring, while the recovery rate of M. canis from cats was very significantly higher in fall and winter than in summer and spring.