Journal ArticleDOI
Using electrophoretic deposition to identify protein charge in biological medium
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TLDR
In this study, electrophoretic deposition was used as a simple method to identify the charge of proteins present in biological medium and the suitability of EPD of proteins to deliver tailored surfaces for enhanced bioactivity or for controlled deposition of protein films on metallic surfaces was explored.Abstract:
Protein adsorption is the first step involved in establishing a suitable integration between a biomaterial and host tissue. It is therefore of highest interest to know the electric charge of proteins present in the relevant medium to be able to predict the behaviour of cells on given surfaces. In this study, electrophoretic deposition (EPD) was used as a simple method to identify the charge of proteins present in biological medium. In the model experiment carried out here, EPD was conducted using a biological medium containing 10% fetal calf serum (FCS) and the charge of the protein was determined by examining the migration of the protein in the EPD cell under a certain applied voltage. In addition, the suitability of EPD of proteins to deliver tailored surfaces for enhanced bioactivity or for controlled deposition of protein films on metallic surfaces was explored.read more
Citations
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Journal ArticleDOI
Electrophoretic deposition of biological macromolecules, drugs, and cells.
Sigrid Seuss,Aldo R. Boccaccini +1 more
TL;DR: An overview of the available literature on the application of EPD to process different biomolecules and biological entities, like proteins, bacteria cells, hyaluronic acid, and therapeutic drugs, aiming at using such biomaterials in numerous applications ranging from biosensors to orthopedic implants, tissue scaffolds, and drug delivery devices are given.
Journal ArticleDOI
Electrophoretic Coating of Octahedral Molybdenum Metal Clusters for UV/NIR Light Screening
Thi Kim Ngan Nguyen,Benjamin Dierre,Fabien Grasset,Noée Dumait,Stéphane Cordier,Pierric Lemoine,Adèle Renaud,Hiroshi Fudouzi,Naoki Ohashi,Tetsuo Uchikoshi +9 more
TL;DR: In this paper, two kinds of polydimethylsiloxanes (PDMS) were selected to topcoat the Mo6 cluster film after the electrophoretic deposition (EPD) process, and the influence of the PDMS on the durability, chemical compatibility and light absorption property of Mo6 clusters were characterized by means of field-emission scanning electron microscopy (FE-SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscope (FT-IR), and ultraviolet-visible-near infrared (UV-Vis-NIR)
Journal ArticleDOI
Electrophoretic deposition of cobalt oxide nanoparticles on aluminium substrate
TL;DR: In this article, the effects of deposition time and applied voltage on the quality of cobalt nanoparticle film, deposited on aluminium substrate have been investigated experimentally, and the results showed that the effect of applied voltage and deposition time on the performance of the nanoparticles was negligible.
Dissertation
Biosynthesis of polyhydroxyalkanoates and their medical applications
TL;DR: A multifunctional P(3HB)/45S5Bioglass® composite system for bone tissue engineering was developed which could exhibit topographical features to increase cell attachment and can also act as a carrier for controlled drug delivery via the immobilization of P( 3HB) microspheres on the scaffold surfaces.
Journal ArticleDOI
Characterization of gold nanoparticle thin film prepared by electrophoretic deposition method
TL;DR: In this paper, gold nanoparticles were synthesized, using pulsed laser ablation method of a high-purity gold target in distilled water, and five samples of gold nanoparticle thin films were prepared at different deposition times and applied voltages.
References
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Journal ArticleDOI
Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential
TL;DR: The results demonstrate that electrostatic interactions can play an important factor in protein adsorption and cellular uptake of nanoparticles.
Journal ArticleDOI
Electrophoretic deposition of biomaterials
TL;DR: How EPD has become an important tool in advanced biomaterials processing, as a convenient alternative to conventional methods, and the potential of the technique to manipulate and control the deposition of a range of nanomaterials of interest in the biomedical and biotechnology fields are presented.
Journal ArticleDOI
Basic reactions of osteoblasts on structured material surfaces.
TL;DR: This paper gives a review of present knowledge on the various stages of osteoblast reactions on material surfaces, focused on basic cell events under in vitro conditions, with special emphasis on cellular reactions towards ordered nano-sized topographies.
Journal ArticleDOI
Electrophoretic deposition of chitosan
TL;DR: In this article, the electrophoretic mobility of chitosan in aqueous solution as a function of pH was studied, and the growth rate was estimated to vary in the range 0.02-0.08 µm/s depending on the pH value.
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