Variability and genetic structure of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) in Colombia using microsatellite DNA markers
John Ocampo,Natali Acosta-Barón,Javier Hernández-Fernández +2 more
- Vol. 35, Iss: 2, pp 135-149
TLDR
A complementary agro-morphological assessment is suggested to establish total genetic variability and implement a breeding program through assisted selection of superior genotypes in search of more productive and resistant cultivars to phytosanitary problems.Abstract:
Colombia is one of the leading producers of yellow passion fruit but the genetic studies based on molecular markers from commercial plantations have not been considered to select interesting market material. The goal of this study was to assess the genetic variability and the population structure of 51 Colombian commercial yellow passion fruit accessions (102 individuals), and to provide the necessary information for prospective selection and breeding programs. Thus, a total of six microsatellites were amplified with 58 alleles identified and an average of 9.66 alleles per locus, including nine private and 31 rare. Diversity indexes showed polymorphic information content values of 0.74 (PIC), an observed (Ho) and expected (He) heterozygosity average of 0.52 and 0.78, respectively. Spatial distribution showed the greatest allelic richness (11 to 14) in most of the Valle del Cauca accessions. The average genetic distance among accessions was 0.68, and the cluster analysis showed three main groups poorly supported (bootstrap <50%), with slight geographical structure and high differentiation between individuals of the same accession. Structure analysis indicated K=4 as the genetic structure's uppermost hierarchical level, while Bayesian clustering showed a division of individuals into four genetically distinct groups. The low geographic structure and high variability of the accessions could be explained by allogamy and seed exchange frequency among farmers. Results issued suggest a complementary agro-morphological assessment to establish total genetic variability and implement a breeding program through assisted selection of superior genotypes in search of more productive and resistant cultivars to phytosanitary problems.read more
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Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits.
TL;DR: In this paper, the authors evaluated alternatives and specific F-C reacting conditions aiming at determining the highest total phenolic content (TPC) in three Colombian Passifloras.
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Genotyping-by-sequencing of passion fruit (Passiflora spp.) generates genomic resources for breeding and systematics
Joshua D. Anderson,Ravena Ferreira Vidal,Maria Brym,Eric T. Stafne,Marcio F. R. Resende,Alexandre Pio Viana,Alan H. Chambers +6 more
TL;DR: This study developed single nucleotide polymorphism (SNP) markers to characterize 59 accessions from 21 species that included native and naturalized Passiflora species found in Florida, USA, which could aid the genetic improvement of passion fruit in the future.
Agro-morphological characterization of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) reveals elite genotypes for a breeding program in Colombia
TL;DR: The aim of this research was to establish the genetic relationships among accessions of different geographic origins through an agro-morphological characterization ofYellow passion fruit cultivation in Colombia to obtain more productive and tolerant cultivars.
Journal ArticleDOI
Molecular diversity assessment among the pomegranate genotypes belonging to diverse genetic background using microsatellite markers
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