Journal ArticleDOI
Yeast cell-cycle mutant cdc21 is a temperature-sensitive thymidylate auxotroph
TLDR
Genetic tests with the yeast cell-cycle mutantcdc21 isolated by Hartwell indicate that the CDC21 gene in yeast is the same as the TMP1 gene, whose mutant alleles confer an auxotrophic requirement for thymidine-5′-monophosphate (dTMP).Abstract:
Genetic tests with the yeast cell-cycle mutantcdc21 isolated by Hartwell indicate that the CDC21 gene in yeast is the same as the TMP1 gene, whose mutant alleles confer an auxotrophic requirement for thymidine-5′-monophosphate (dTMP). Yeast strains carryingcdc21 can grow at 37° in the presence of dTMP provided that they are permeable to this compound. The gene is shown to be linked toade2 on Chr. XV, and a case of intragenic complementation betweencdc21 and anothertmp1 allele is reported.read more
Citations
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Journal ArticleDOI
Genetic map of Saccharomyces cerevisiae.
R K Mortimer,D Schild +1 more
TL;DR: Although all of the data presented in this article are from tetrad analyses, many other techniques have been used to assign genes to chromosomes or to specific chromosome arms, including aneuploid analysis, mitotic recombination analysis,Mitotic chromosome loss or nondisjunction, and random spore analysis.
Journal ArticleDOI
Thymine metabolism and thymineless death in prokaryotes and eukaryotes
TL;DR: A model is presented for a molecular basis of TLD and suggests that the RecF repair pathway may be critical to cell death, perhaps because it increases the occurrence of double-strand DNA breaks with unique DNA configurations at lesion sites.
Journal ArticleDOI
Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase
Leland H. Johnston,Kim Nasmyth +1 more
TL;DR: It is reported here that cdc9–l, isolated by Hartwell as a conditional cell cycle mutant, is defective in a DNA ligase involved in both replication and repair of DNA.
References
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Journal ArticleDOI
Genetic Control of the Cell Division Cycle in Yeast
TL;DR: Two features which distinguish the cell cycle of Saccharomyces cerevisiae from most other eukaryotes are particularly useful for an analysis of the gene functions that control the cell division cycle.
Journal ArticleDOI
The Saccharomyces cerevisiae Cell Cycle
TL;DR: The bibliography is intended more as a guide to the literature than as a historically accurate record of the development of the field; the authors apologize to the earlier workers whose contributions thus get less explicit credit than they deserve.
Journal ArticleDOI
GENETIC CONTROL OF THE CELL DIVISION CYCLE IN YEAST: V. GENETIC ANALYSIS OF cdc MUTANTS
TL;DR: The gene products that are defined by the cdc cistrons are essential for the completion of the cell cycle in haploids of a and alpha mating type and in a/alpha diploid cells and the same genes control thecell cycle in each of these stages of the life cycle.
Journal ArticleDOI
Genetic Control of the Cell-Division Cycle in Yeast, I. Detection of Mutants
TL;DR: Time-lapse photomicroscopy has been utilized to detect temperature-sensitive yeast mutants that are defective in gene functions needed at specific stages of the cell-division cycle to provide two types of information about a mutant: the time at which the defective gene function is normally performed, and the stage at which cells collect when the function is not performed, defined as the termination point.
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Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase
Leland H. Johnston,Kim Nasmyth +1 more