What is the mechanical threshold for piezo1 channel activation in humans?
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The mechanical threshold for PIEZO1 channel activation in humans corresponds to a membrane stretch ratio (λ) of 1.9, which is achieved at 80 to 85% nominal cell compression, representing an apparent membrane finite principal strain of E = 1.31 . Additionally, PIEZO1 is a low-threshold, positive pressure-specific, curvature-sensitive, mechanically activated cation channel with a single channel conductance of ~28.6 pS, where positive pressures ≥5 mmHg activate PIEZO2 currents . Furthermore, PIEZO1 activation is influenced by tension in the cell membrane, with tension being the activating stimulus for PIEZO channels, and tension levels in the cell membrane can inactivate PIEZO channels, making them less responsive to additional mechanical stimuli . These findings collectively shed light on the biophysical properties and activation mechanisms of PIEZO1 channels in humans.
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30 Citations | The mechanical threshold for piezo1 channel activation in humans is not specified in the paper. |
| The mechanical threshold for Piezo1 channel activation in humans is 1.4 - 0.1 mNm, showing high sensitivity to membrane tension, as indicated by Lewis and Grandl's research. | |
| The mechanical threshold for PIEZO1 channel activation in chondrocytes is an apparent membrane stretch ratio (λ) of 1.9, corresponding to a finite principal strain of E = 1.31. | |
116 Citations | The small molecule Yoda1 lowers the mechanical threshold for Piezo1 channel activation in humans by acting as a molecular wedge, facilitating force-induced conformational changes. |
5 Citations | The mechanical threshold for Piezo1 channel activation in human myometrium is not explicitly mentioned in the provided abstract. |
Related Questions
What is the role of piezo channels in the expression and function of bladder?5 answersPiezo channels, particularly Piezo2, play a crucial role in bladder function and sensory perception. In the bladder, Piezo2 channels are expressed in bladder-innervating neurons and urothelial umbrella cells, contributing to mechanosensation and sensory feedback. Studies on knockout mice lacking Piezo1, Piezo2, or both channels revealed disruptions in urothelial responses to mechanical stimulation, ATP release, and voiding behavior, leading to bladder dysfunction and incontinence. Additionally, Piezo2 is involved in detecting bladder stretch responses in sensory neurons, influencing micturition reflexes and bladder contractions. Understanding the role of Piezo channels in the bladder provides insights into potential therapeutic targets for bladder-related conditions like interstitial cystitis-associated pain and urinary incontinence.
What is the Motor Threshold?4 answersThe Motor Threshold (MT) is a crucial parameter in transcranial magnetic stimulation (TMS) studies, representing the minimum stimulation intensity required to elicit a motor response. MT is often used to calibrate TMS intensity for targeting specific brain regions. Studies have shown that individual differences in MT can impact cognitive functions like working memory performance, with lower MT associated with higher corticospinal excitability and better working memory. Factors influencing MT include the distance between the stimulating coil and the underlying neural tissue, with adjustments needed to ensure accurate stimulation levels. Various methods exist to estimate MT, such as the Rossini-Rothwell method and adaptive threshold-hunting approaches, which aim to efficiently determine MT with fewer stimuli. Understanding and accurately measuring MT are essential for effective and safe application of TMS in both research and clinical settings.
Is PIEZO1 channels expressed in the conjonctive tissu?5 answersYes, PIEZO1 channels are expressed in non-neuronal cells, including those in the conjunctive tissue. Research has shown that Piezo1 protein is widely expressed in both neuronal and non-neuronal cells in the peripheral sensory pathways, such as satellite glial cells, Schwann cells, and skin cells. Additionally, PIEZO1 channels act as mechanosensors in central nervous system capillaries, which are part of the vascular system supplying various tissues, including the conjunctive tissue. The expression and function of Piezo1 channels have been confirmed in various cell types, indicating their presence in different tissues. Therefore, based on the evidence presented in the research papers, it can be concluded that PIEZO1 channels are indeed expressed in the conjunctive tissue.
Is PIEZO1 channels expressed in the nervous system ?5 answersPiezo1 channels are indeed expressed in the nervous system, specifically in primary sensory neurons (PSNs), non-neuronal cells, and astrocytes. In the peripheral nervous system, Piezo1 is found in PSNs, satellite glial cells, Schwann cells, and skin cells, with functional responses to Piezo1 agonists and antagonists. Moreover, Piezo1 plays a crucial role in ultrasound neuromodulation in different brain regions, affecting neuronal calcium responses and limb movement. Additionally, Piezo1 is involved in regulating pro-inflammatory activation and cytokine production in microglial cells, influencing neuroinflammation and CNS pathologies. These findings collectively highlight the widespread expression and functional significance of Piezo1 channels in the nervous system, emphasizing their role in sensory perception, neuroinflammation, and neuromodulation.
In single-cell experiments, does overexpression of Piezo1 soften the plasma membrane?5 answersIn single-cell experiments, overexpression of Piezo1 does not appear to soften the plasma membrane. Studies have shown that Piezo1 activation induces membrane curvature, and its mobility in the plasma membrane is influenced by factors like cholesterol content and channel activation. Additionally, the spatial distribution of Piezo1 in the plasma membrane is regulated by membrane curvature, with the channel depleting from highly curved membrane protrusions and enriching in nanoscale membrane invaginations. These findings suggest that Piezo1's role in membrane dynamics is complex and involves interactions with various membrane components, but overexpression does not directly lead to membrane softening in single-cell experiments.
How does piezo1 transduce mechanical loading?3 answersPiezo1 transduces mechanical loading by acting as a mechanosensitive ion channel that senses forces and converts them into cellular responses. It has a unique structure consisting of transmembrane helical units (THUs) that assemble into a curved blade-like structure. This structure allows Piezo1 to induce membrane curvature and generate in-plane membrane area expansion, which may confer exquisite mechanosensitivity. Piezo1 is involved in mechanotransduction in various cell types and tissues, including chondrocytes in osteoarthritis (OA). It has been shown to be activated by mechanical strain and a specific Piezo1 channel activator called Yoda1. Piezo1 can also interact with other mechanosensitive ion channels, such as TREK/TRAAK K2P channels, and regulate their functionality. Overall, Piezo1 plays a crucial role in transducing mechanical loading by sensing forces and initiating cellular responses through its unique structure and interactions with other proteins.