What techniques can be used to characterise the effect of interleukin from macrophage?
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Interleukin (IL) effects on macrophages can be characterized using several techniques. One approach is to evaluate the production of IL1 by monocytes in the presence of different biomedical polymers, which can be used as a model system for studying macrophage activation . Another technique involves investigating the impact of IL-4 on nitric oxide (NO) production by macrophages. This can be done by priming macrophages with IL-4 and then stimulating them with interferon-gamma (IFN-γ) and/or lipopolysaccharide (LPS) to measure NO2 accumulation . Additionally, IL-4 has been shown to induce apoptosis of acute myeloid leukemia (AML) cells in a Stat6-dependent manner, and IL-4-induced macrophages have been characterized using RNA sequencing and gene expression analysis . IL-10 has also been studied for its effect on NO production by macrophages, with differential responses observed depending on the macrophage population . Overall, these techniques provide insights into the role of ILs in modulating macrophage function and can help in understanding their therapeutic potential in various diseases.
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28 Citations | The techniques used in this study to characterize the effect of interleukin on macrophages included measuring nitric oxide production and tumor necrosis factor-alpha levels. |
24 Citations | The paper does not mention specific techniques used to characterize the effect of interleukin-6 on macrophages. |
45 Citations | Flow cytometry can be used to characterize the effect of interleukin from macrophages. |
2 Citations | Flow cytometry analysis and RNA sequencing can be used to characterize the effect of interleukin on macrophages. |
177 Citations | In vitro cell culture techniques can be used to evaluate the effect of interleukin from macrophages. |
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