Showing papers on "2,3-Butanediol published in 2019"

Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Abstract: BACKGROUND: 2,3‐butanediol is a chemical component with wide industrial applications, such as biofuels and chemical precursors. Reducing 2,3‐butanediol production costs is a high priority and recent focus has been on its biological synthesis from raw renewable materials rather than through conventional chemical procedures. In this study, a 2,3‐butanediol native bacterial producer was selected for the fermentation of Agave cupreata bagasse waste by enzymatic hydrolysation to obtain 2,3‐butanediol. RESULTS: The native Klebsiella oxytoca UM2‐17 strain was selected to efficiently produce 2,3‐butanediol on synthetic mineral media. Using glucose (100 g L⁻¹) and glucose: xylose (50 g L⁻¹:50 g L⁻¹) as sugar sources obtained titres of 29.36 g L⁻¹ and 25.9 g L⁻¹ of 2,3‐butanediol, corresponding to yields of 0.29 g g⁻¹ and 0.26 g g⁻¹, respectively. Agave bagasse solid waste after acid pre‐treatment was utilized to obtain enzymatic hydrolysates, obtaining liquors with glucose/xylose/arabinose at 25.8 g L⁻¹ with efficiency of ∼52.5% sugar conversion from residual solid pre‐hydrolysed bagasse. The production of 2,3‐butanediol through batch fermentation of the enzymatic‐hydrolysate by the UM2‐17 strain showed total depletion of glucose and xylose, achieving a maximal production of 10.3 g L⁻¹ of 2,3‐butanediol plus 0.28 g L⁻¹ ethanol, corresponding to 2,3‐butanediol yield of 0.40 g g⁻¹. CONCLUSION: The results indicate that A. cupreata bagasse waste from mezcal beverage elaboration is a sustainable bioresource for production of 2,3‐butanediol by fermentation of enzymatic bagasse‐hydrolysate using the K. oxytoca UM2‐17 strain. © 2019 Society of Chemical Industry

Screening of a highly inhibitor-tolerant bacterial strain for 2,3-BDO and organic acid production from non-detoxified corncob acid hydrolysate

Abstract: Fermentation of chemicals from lignocellulose hydrolysate is an effective way to alleviate environmental and energy problems. However, fermentation inhibitors in hydrolysate and weak inhibitor tolerance of microorganisms limit its development. In this study, atmospheric and room temperature plasma mutation technology was utilized to generate mutant strains of Enterobacter cloacae and screen for mutants with high inhibitor tolerance to acid hydrolysate of corncobs. A highly inhibitor-tolerant strain, Enterobacter cloacae M22, was obtained after fermentation with non-detoxified hydrolysate, and this strain produced 24.32 g/L 2,3-butanediol and 14.93 g/L organic acids. Compared with that of the wild-type strain, inhibitor tolerance was enhanced twofold with M22, resulting in improvement of 2,3-butanediol and organic acid production by 114% and 90%, respectively. This work presents an efficient method to screen for highly inhibitor-tolerant strains and evidence of a novel strain that can produce 2,3-butanediol and organic acids using non-detoxified acid hydrolysate of corncobs.

Cloning, expression and characterization of a novel (2R,3R) -2,3-butanediol dehydrogenase from Bacillus thuringiensis

Abstract: Acetoin (3-hydroxy-2-butanone) is an important four-carbon compound widely used in the food industry and other industrial applications. This study aimed to identify a novel butanediol dehydrogenase that can efficiently catalyze the formation of acetoin. A novel butanediol dehydrogenase, BtBDH, was identified from Bacillus thuringiensis subsp. Kurstaki ACCC 10066, and its enzymatic properties were characterized. The optimum pH and temperature for the oxidation activity of BtBDH were 10.0 and 50 °C, respectively, and those for the reduction activities were 7.5 and 35 °C, respectively. In addition, it exhibits stability over a wide pH range (6–10) and temperatures up to 70 °C. BtBDH showed good stability after storage for 3 months at 4 °C. Moreover, ethylenediaminetetracetic acid (EDTA) inhibits the enzymatic activity of BtBDH, indicating that the enzyme is metal-dependent. This study characterized a novel (2R,3R) −2,3-butanediol dehydrogenase. Its excellent oxidation activity and stability ensure its great industrial application potential in the production of acetoin.

Procedure for obtaining 2,3-butanediol by fermentation of hydrolyzed substrates of organic waste (ow) with microorganisms

Abstract: The present invention refers to a procedure for the obtention of compound 2,3-Butanediol by the fermentation of the hydrolyzed substrates of solid or liquid waste of any origin, with high or medium contents of organic matter: Organic waste (hereinafter OW) using microorganisms, particularly by using a strain of Raoultella planticola.

Strain and method for producing 2,3-butanediol and organic acid

Abstract: The invention provides a microorganism and a method for obtaining 2,3-butanediol and organic acid. The microorganism is enterobacter cloacae M22 deposited on China General Microbiological Culture Collection Center on February 26, 2019 with the deposit number of CGMCC NO. 17265. The microorganism can achieve high tolerance to an inhibitor by utilizing a lignocellulose hydrolyzate not detoxified, and can also produce the 2,3-butanediol and the organic acid with high yield. Moreover, the microorganism has good stability, continuous passage for many times, tolerance to the inhibitor and stable generation capability of the 2,3-butanediol and the organic acid by fermentation. In addition, detoxification of the hydrolyzate increases the process cost, and some chemical reagents used in the detoxification process cannot be recycled. Direct use of the hydrolyzate not detoxified for fermentation greatly reduces the production cost of the 2,3-butanediol and the organic acid, and good application prospects can be achieved.