Showing papers on "Campesterol published in 1984"
TL;DR: A method for separating and quantifying seed oil steryl esters and free sterols was developed using a combination of preparative column, thin layer (TLC), and gas liquid chromatography (GLC) as discussed by the authors.
Abstract: A method for separating and quantitating seed oil steryl esters and free sterols was developed using a combination of preparative column, thin layer (TLC), and gas liquid chromatography (GLC). Cholesteryl heneicosanoate and cholesterol served as internal standards. The method was applied to corn-oil samples (Mazola, Kroger) obtained from the local market and peanut-oil samples prepared in the laboratory from commercial varieties of peanuts (Florunner, Starr). Concentration (mg/100 g oil; mean ± SD) of steryl esters and free sterols in the 4 oils were: Mazola, 1420±40 and 370±8; Kroger, 950±40 and 320±4; Florunner, 74±0.5 and 150±3; and Starr, 51±0.5 and 130±2. Sitosterol was the major sterol in both the free sterol and steryl ester fractions of all oils and together with campesterol, stigmasterol and Δ5-avenasterol made up 90–95% of all sterols. Steryl esters of peanut oil contained higher proportions of linoleic acid and long-chain acids (C20–C24) than did whole oil. Corn-oil steryl esters also contained a higher proportion of linoleic acid than did whole oil. Squalene was the major hydrocarbon of all oils with the remaining hydrocarbon fraction consisting of a mixture of compounds.
54 citations
TL;DR: This is the first time codisterol has been found in a higher plant and also the firsttime the structures and configurations of the Δ 5 -sterols from a Cucurbitaceae species have been clearly characterized.
44 citations
TL;DR: The fatty acid composition of a new strain of Rhodotorula glutinis isolated from soil and grown on molasses found to be close to that of palm oil.
Abstract: The fatty acid, sterol and hydrocarbon compositions of oil produced by a new strain of Rhodotorula glutinis isolated from soil and grown on molasses, were determined. The major constituent fatty acids were: palmitic (37%) oleic (47%) and linoleic (8%). The major sterols were campesterol (42%) and stigmasterol (27%) and the major hydrocarbons were n-C23 (37%), iso-C29 (30%) and n-C21 (19%). Minor constituents in each class of lipids were also detected and estimated. The oil produced was 54% on a dry weight basis. The fatty acid composition was found to be close to that of palm oil.
37 citations
Patent•
04 Sep 1984
TL;DR: In this article, a set of emollients of soy bean derived sterols, such as acetylated sitosterol, acetylate campesterol, and stigmasterol, are provided as emollient for skin treatment compositions.
Abstract: Cosmetic and skin treatment compositions are provided which include as emollients acetylated soy bean derived sterols, namely, acetylated sitosterol, acetylated campesterol, and/or acetylated stigmasterol The above-acetylated sterols impart improved hand feel and barrier properties to the compositions, are naturally compatible with skin and have improved skin penetration properties over unesterified sterols
33 citations
TL;DR: The effects of triarimol, tridemorph and triparanol on sterol biosynthesis in carrot, tobacco and soybean suspension cultures were studied and resulted in the accumulation of 14α-methyl sterols in carrot and tobacco cultures.
Abstract: The effects of triarimol, tridemorph and triparanol on sterol biosynthesis in carrot, tobacco and soybean suspension cultures were studied. The 3 plant species normally contain campesterol, stigmasterol and sitosterol as major sterols. Triarimol inhibited demethylation at C 14 and the second alkylation of the side chain in all 3 species. The primary effects of tridemorph were the inhibition of the opening of the 9β,19-cyclopropane ring and the second alkylation of the side chain. Triparanol treatments resulted in the accumulation of 14α-methyl sterols, and the inhibition of second alkylation in the side chain in carrot and tobacco cultures. Cyclopropyl sterols also accumulated in carrot and tobacco cultures treated with triparanol. Triparanol did not alter the sterol composition of soybean cultures except for decreasing concentrations of campesterol and stigmasterol and increasing amounts of sitosterol.
32 citations
TL;DR: The results obtained by carrying out plate lipolysis for 45 min at 40° compared well with those produced by conventional chemical hydrolysis.
31 citations
TL;DR: In this article, the kernel fat content of 16 different mango varieties collected from the Northwestern part of Madagascar island were examined, and the main sterols were β-sitosterol (47−76%), stigmasterol (12−23%), and campesterol (7−12%).
Abstract: The kernel fat content of 16 different mango varieties collected from the Northwestern part of Madagascar island were examined. The fat content (22–54%) was determined by chloroform/methanol extraction. Investigation by gas liquid chromatography (GLC) revealed 15 fatty acids, mainly palmitic (7–12%), stearic (22–40%), oleic (41–48%) and linoleic (7–17%). Significant correlations were observed among the main fatty acids. Testing for the sterol fraction in 15 mango varieties allowed us to separate and quantitatively analyze 7 sterols by GLC. The main sterols wereβ-sitosterol (47–76%), stigmasterol (12–23%) and campesterol (7–12%). The stigmasterol/campesterol ratio (1.2:2.3) was lower in mango kernel fat than in cocoa butter. Among the 4-methyl sterol fractions, gramisterol, lophenol, obtusifoliol and citrostadienol were tentatively identified by GLC. Lupeol, cycloartenol,α- andβ-amyrins and friedelinol were tentatively identified by GLC in the triterpene alcohols fractions.
23 citations
Journal Article•
TL;DR: The sterol fraction of Equisetum arvense L. contains, essentially, the following sterols: beta-sitosterol (60.0%), campesterol (32.9%), isofucosterol(5.9%) and cholesterol (trace amounts).
Abstract: The sterol fraction of Equisetum arvense L. contains, essentially, the following sterols: beta-sitosterol (60.0%), campesterol (32.9%), isofucosterol (5.9%) and cholesterol (trace amounts). The identification of the compounds has been carried out through NMR and MS, while the corresponding percentage have been desumed from the GLC and HPLC data.
23 citations
TL;DR: The effects of feeding diets with high or low amounts of cholesterol and with low or high levels of mixed plant sterols on the daily fecal excretion of acidic steroids were studied in rhesus monkeys and suggest that the effect of cholesterol feeding on fecal acidic steroid excretion depends on the level of plant sterol in the diet.
18 citations
TL;DR: Various parts of the winged bean plant, Psophocarpus tetragonolobus, were analyzed for sterols and fatty acids and the major sterols were sitosterol, stigmasterol and campesterol.
Abstract: Various parts of the winged bean plant, Psophocarpus tetragonolobus were analyzed for sterols and fatty acids. The major sterols were sitosterol, stigmasterol and campesterol. Sitosterol occurred in greatest amounts except in roots where stigmasterol was the predominant sterol. In seeds, behenic acid (22:0) comprised 13.5% of the total fatty acids, whereas, in the other plant parts it varied from 0.1–6.9%. Leaves contained the highest level of fatty acids, but only 22% of these fatty acids were saturated.
15 citations
TL;DR: In seeds and leaves of oats (Avena sativa L.) 12 different sterols have been identified and the sterol pattern is qualitatively the same, but the relative composition is different in leaves and in seeds.
Abstract: In seeds and leaves of oats (Avena sativa L.) 12 different sterols (cholesterol, cholstanol, Δ7-cholestenol, campesterol, campestanol, stigmasterol, lophenol, sitosterol, stigmastanol, Δ5-avenasterol, Δ7-avenasterol and Δ7-stigmastenol) have been identified. The sterol pattern is qualitatively the same, but the relative composition is different in leaves and in seeds. Leaves contain mainly sitosterol, stigmasterol, cholesterol and campesterol, but only minor portions of avenasterols. Seeds contain sitosterol, Δ5- and Δ7-avenasterol, campesterol, but only minor amounts of stigmasterol and cholesterol. In leaf lipids 1-hexacosanol (2.35 wt % of total lipid) has also been identified.
TL;DR: The 13C labeling patterns of the two methyl groups at C-25 of dihydrobrassicasterol biosynthesized from [1,2-13C2]acetate differ from those of campesterol and 24-methylenecholesterol obtained from cultured cells of Physalis peruviana and ergosterol from yeast as mentioned in this paper.
Abstract: The 13C labelling patterns of the two methyl groups at C-25 of dihydrobrassicasterol biosynthesized from [1,2-13C2]acetate differ from those of campesterol and 24-methylenecholesterol obtained from cultured cells of Physalis peruviana and ergosterol from yeast.
TL;DR: In this article, the sterol groups were separated as 4-desmethylsterol, 4-monomethylsterol and 4, 4'-dimethylsterol by TLC, and their components were determined by GLC.
Abstract: Refined commercially available vegetable oils (soybean, corn, rapeseed and rice bran oils) were heated in glass tubes for AOM test at 200°C for 30h (6h/d), and the changes in the unsaponifiables and desmethylsterol content in these oils were investigated. Individual sterol groups were separated as 4-desmethylsterol, 4-monomethylsterol and 4, 4'-dimethylsterol by TLC, and their components were determined by GLC. The percentage of unsaponifiable matter in the oils was fairly constant during heating, but that of 4-desmethylsterol decreased considerably. In general, the percentage of sitosterol in the 4-desmethylsterol, cycloeucalenol in the 4-monomethylsterol and β-amyrin in the 4, 4'-dimethylsterol, increased with heating. However, the percentage of campesterol and stigmasterol in the 4-desmethylsterol as well as that of obtusifoliol and citrostadienol in the 4-monomethylsterol decreased steadily.