Showing papers on "genomic DNA published in 1977"
TL;DR: Evidence is presented that XIt 5S RNA is transcribed in ovaries but not in somatic cells; XIt5S DNA may be under the same control as the major oocyte 5S DNA.
44 citations
TL;DR: The results suggest that the mRNA is not grossly contaminated with non-mRNA sequences and thus the method may be used to obtain mDNA for use in experiments where mDNA excess hybridizations are required.
30 citations
TL;DR: Complementary DNA was prepared with viral RNA-dependent DNA polymerase using human globin messenger RNA (mRNA) as template, and the rate and extent of hybridization confirmed that there are two genes for alpha-globin per haploid genome.
Abstract: Complementary DNA (cDNA) was prepared with viral RNA-dependent DNA polymerase using human globin messenger RNA (mRNA) as template. By selective hydridization to globin mRNA from beta-thalassaemics a probe which was greater than 85% complementary to alpha-globin mRNA was purified. This was hybridized in cDNA excess to human genomic DNA, and the rate and extent of hybridization confirmed that there are two genes for alpha-globin per haploid genome. Cellular DNA was also prepared from peripheral blood from cases expressing the alpha-globin chain mutant Hb J Mexico to varying extents. This DNA was identical in hybridization behaviour to normal DNA demonstrating that the imbalanced mutant chain synthesis seen physiologically is not due to a gene deletion.
14 citations
TL;DR: Native DNA components in the human genome were identified with an improved RNA/DNA gradient hybridization technique and three distinct radioactive peaks were found at the heavy buoyant densities, suggesting a differential enrichment of DNA components with sequences complementary to chromosomal RNA.
2 citations