Showing papers on "Isovitexin published in 2012"
TL;DR: Both the C-glycosyl bioflavonoids, namely, vitexin and isovitexIn exhibited in vivo α-glucosidase inhibition.
188 citations
TL;DR: In this article, the authors measured the antioxidant capacities of the soup prepared with three cultivars of mung bean and found that the two major flavonoids in the MBS were purified and identified as vitexin and isovitexins, respectively.
55 citations
TL;DR: E. cellulosolvens cleaved the flavone C-glucosides homoorientin and isovitexin to their aglycones luteolin and apigenin, respectively, and several O-coupled glucosides of flavones and isoflavones to their corresponding agly Cones.
Abstract: Eubacterium cellulosolvens cleaved the flavone C-glucosides homoorientin and isovitexin to their aglycones luteolin and apigenin, respectively. The corresponding isomers, orientin and vitexin, or other polyphenolic C-glucosides were not deglycosylated. E. cellulosolvens also cleaved several O-coupled glucosides of flavones and isoflavones to their corresponding aglycones.
52 citations
TL;DR: The results indicated that the free radical scavenging activity of orientin and isoorientin are stronger than those of two antioxidative drugs, vitamin C and baicalin.
46 citations
TL;DR: Introducing an acyl group into isoorientin and isovitexin significantly improved their lipophilicity but reduced their antiradical activity.
Abstract: This study enzymatically acrylates two flavonoids from bamboo-leaf extracts, isoorientin and isovitexin, with different fatty acids as acyl donors using Candida antarctica lipase B (CALB). The conversion yield ranged from 35 to 80% for fatty acids with different chain lengths. Higher isoorientin and isovitexin conversion yields (>75%) were obtained using lauric acid in tert-amyl-alcohol as the reaction medium. 1H and 13C nuclear magnetic resonance spectroscopy analysis showed that, in the presence of CALB, acylation occurred at the isoorientin and isovitexin primary hydroxyl group of glucose moiety and only monoesters were detected. Introducing an acyl group into isoorientin and isovitexin significantly improved their lipophilicity but reduced their antiradical activity.
42 citations
TL;DR: The proposed method was validated to be simple and reliable and can be a tool for quality control of bamboo leaf extract or its commercial products.
Abstract: A strategy for analyzing flavone C-glucosides in the leaves of different species of bamboo was developed. Firstly, the flavone C-glycosides were extracted from the bamboo leaves (51 species in 17 genera) with methanol and chromatographed on silica gel 60 plates in automatic developing chamber (ADC2), and a qualitative survey using simple derivatization steps with the NP reagent was carried out. The flavone C-glycosides were found in 40 of 51 species of bamboo examined. Secondly, an HPLC method with photodiode array and multiple wavelength detector was optimized and validated for the simultaneous determination of flavone C-glycosides, including isoorientin, isovitexin, orientin, and vitexin in the leaves of three species of bamboo and the flavone C-glycosides were confirmed by LC/MS. The optimized HPLC method proved to be linear in the concentration range tested (0.2–100 μg/mL, ), precise (%), and accurate (88–106%). The concentration ranges of isoorientin, isovitexin, orientin, and vitexin in three bamboo leaves samples were 1.00–2.78, 0–0.31, 0–0.07, and 0.20–0.68 mg/g, respectively. The proposed method was validated to be simple and reliable and can be a tool for quality control of bamboo leaf extract or its commercial products.
41 citations
TL;DR: Four flavone C-glycosides were isolated from the neotropical blueberry of Anthopterus wardii using antioxidant activity-guided fractionation and exhibited inhibitory activity against IL-8 production and MMP-1 expression, with compounds 1, 3, and 4 having the most potent inhibitory activities in both assays.
28 citations
TL;DR: A seventy percent ethanol from mung bean was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions that showed the highest inhibitory activity when using l-3,4-dihydroxyphenylalanine as the substrate for mushroom tyrosinase.
Abstract: A seventy percent ethanol from mung bean (Vigna radiatae L.) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. When using l-3,4-dihydroxyphenylalanine as the substrate for mushroom tyrosinase, the EtOAc-soluble fractions showed the highest inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guided fractionation method) from the EtOAc-soluble fractions. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 6.3 and 5.6 mg/ml, respectively. This is the first study on the active compositions of azuki beans against mushroom tyrosinase.
25 citations
TL;DR: The results confirm the traditional usage of G. lutea leaves and also suggest their possible utilization as hepatoprotective, hypoglycemic and anti-inflammatory agents.
Abstract: LC-ESI-MS and HPLC were used for the identification of the constituents from G. lutea leaves collected at different localities, as well as for quantification of the main compounds. Seven secoiridoids, five C-glucoflavones and three xanthones, were identified. Swertiamarin derivatives, namely eustomorusside (2), eustomoside (3) and septemfidoside (5), were detected in G. lutea for the first time. Concentrations of five constituents (swertiamarin, gentiopicrin, isovitexin, mangiferin and isogentisin) were determined. The relationship between concentrations of y-pyrones and altitude was observed with statistically significant correlation (r = 0.94). The extracts were also evaluated for their content of total phenolics, and antiradical and cytotoxic activities. The total phenolics content ranged from 7.7 to 12.7 mg GAE/g, and the IC50 values for DPPH radical scavenging activity varied between 0.45 to 2.02 mg/mL. The leaf extract exhibited moderate cytotoxic effects toward HeLa cells with an IC50 value of 41.1 microg/mL, while gentiopicrin, mangiferin and isogentisin exerted strong activity against HeLa cells, with IC50 values ranging from 5.7 to 8.8 microg/mL. The results confirm the traditional usage of G. lutea leaves and also suggest their possible utilization as hepatoprotective, hypoglycemic and anti-inflammatory agents.
24 citations
TL;DR: In this article, Wight et al. have shown the α-glucosidase inhibitory and antioxidant properties of Pimpinella candolleana extracts by column chromatography and NMR spectroscopic data.
Abstract: EtOAc and MeOH different extracts of Pimpinella candolleana Wight et Arn. have shown the α-glucosidase inhibitory and antioxidant activities when they were assayed in vitro. Chemical constituents of both extracts were isolated by column chromatography, and identified by MS and NMR spectroscopic data. Nine compounds were isolated, including 3 sterols, 2 flavones, 1 triterpene, 1 glucoside, 1 phenol derivatives, and 1 other compound. Their structures were identified as ursolic acid (1), luteolin (2), urea (3), stigmasta-5,22-dien-3-ol acetate (4), erythrol (5), isovitexin (6), 1-(4-hydroxyphenyl)-1,2-ethanediol (7), daucosterol (8), and β-sitosterol (9). Compound 1 (IC50 = 4.42 μg ml−1), 2 (IC50 = 5.96 μg ml−1), 4 (IC50 = 67.43 μg ml−1) and 6 (IC50 = 68.71 μg ml−1) showed α-glucosidase inhibitory activity. Compound 2 (IC50 = 0.99 μg ml−1) had antioxidant activity. All compounds except for 1 and 9 were isolated from this genus for the first time.
15 citations
TL;DR: In this article, the structure of two C-glycosyl flavones extracted from Jatropha multifida (Euphorbiaceae) leaves were determined through spectroscopic methods, including UV, IR, 1D and 2D NMR and compared with the related known compounds.
Abstract: The ethyl acetate extract from Jatropha multifida (Euphorbiaceae) leaves yielded two C-glycosyl flavones. Their structures were elucidated through spectroscopic methods, including UV, IR, 1D and 2D NMR, and compared with the related known compounds. The structures of the two flavonoids were determined as Vitexin ( 1 ) and Isovitexin ( 2 ). The ethanol extracts of leaves and bark and their fractions did not interfere in the integrity of erythrocytes, not even 1 and 2 . In the Brine shrimp lethality method, bark extracts showed greater toxic potential than the leaf extracts. Both flavonoids are not toxic. The Phosphomolybdenum and DPPH assays were used in order to investigate the antioxidant activity of both compounds and fractions of leaf and bark extracts. The ethyl acetate fraction of bark showed excellent activity, with IC 50 17.23 μg/mL -1 , equivalent to the standard values, Vitamin C and Rutin. Compounds 1 - 2 demonstrated good activity with IC 50 values of 54.37 and 87.27μg/mL -1 . In the Phosphomolybdenum test, the ethyl acetate fraction of bark showed 86.18% of antioxidant activity compared with Rutin, and the chloroform fraction of leaves, 103.29%. In all tests the bark extracts were more bioactive than the leaf extracts.
Journal Article•
TL;DR: A flavonoid glycoside has been isolated from keladi tikus (Typhonium flagelliforme (Lodd) Blume) leaves as discussed by the authors.
Abstract: A flavonoid glycoside has been isolated from keladi tikus (Typhonium flagelliforme (Lodd) Blume) leaves. Keladi tikus is a plant traditionally uses as anticancer. The ethyl acetate extract was isolated using Vacuum Liquid Chromatography, then fractionated with column chromatography and identified by UV-VIS, FTIR, LCMS-MS and 1 H-NMR, 13 C-NMR, 2D-NMR (HMQC, HMBC, DEPT and COSY). The result from the isolation of the ethyl acetate fraction T. flagelliforme (Lodd) Blume obtained 6-glucosyl apigenine namely isovitexin. Isovitexin has antioxidant activity (DPPH free radical scavenging) with IC 50 34.39 µg/mL and cytotoxic activity using BSLT (LC 50 15.84 µg/mL).
TL;DR: The results and previous reports suggest that synergistic effects of flavonoid glycosides are the cause of significant leishmanidal activity of the crude organic extract from K. variabilis leaves.
Abstract: Many phenolic compounds such as xanthones, quinones and coumarins have been isolated from Kielmeyera species; however the presence of flavonoids have been showed in other genera in the Calophylleae tribe as Caraipa, Mesua and Calophyllum. Six known glycosidic flavonoids: quercetin 3-β-O-galactopyranoside (1), quercetin 3-β-O-glucopyranoside (2), quercetin 3-O-α-rhamnoside (3), luteolin 6-C-β-glucopyranoside (4), isovitexin (5), kaempferol 3-O-α-rhamnoside (6) and one triterpene, lupenone (7) were isolated, for the first time, from organic crude extract of Kielmeyera variabilis Mart. & Zucc., Calophyllaceae, leaves. The crude organic extract from K. variabilis leaves exhibited 95% of leishmanidal activity at 20 µg/mL on amastigote-like form of Leishmania (Leishmania) amazonensis in vitro model and only compound 3 showed 40-45% of growth inhibition at concentration ranging from 0.78 to 20 µg/mL. In addition, quercetin 3-O-α-rhamnoside (quercitrin) was found to be the major metabolite. Our results and previous reports suggest that synergistic effects of flavonoid glycosides are the cause of significant leishmanidal activity of the crude organic extract from K. variabilis leaves.
Patent•
26 Dec 2012
TL;DR: The folium microcotis total flavone extract provided by the authors in this paper contains 50 wt% to 95% of the general flavone based on the rutin, and has the obvious effect of adjusting blood fat.
Abstract: The invention discloses a folium microcotis total flavone extract, and a preparation method and an application thereof. The extract contains 50 wt% to 95wt% of general flavone based on rutin, 3.5 wt% to 7.5 wt% of narcissoside in the total flavone extract, 0.5 wt% to 3.0 wt% of vitexin and 0.5 wt% to 3.0 wt% of isovitexin. The preparation method comprises the steps as follows: coarse extracting; taking one or more of water, methanol and ethanol as an extraction solvent for coarse extracting; refining: taking a macroporous resin or a polyamide or a silica gel as an adsorbent, ethanol or other organic solvents as an eluent for eluting, collecting the eluent, and decompressing and concentrating to obtain the folium microcotis total flavone extract. The folium microcotis total flavone extract provided by the invention contains 50 wt% to 95 wt% of the general flavone based on the rutin, and has the obvious effect of adjusting blood fat. The preparation method is simple and effective, thereby being suitable for industrial mass production and capable of preparing medicines for adjusting the blood fat.
TL;DR: Thirteen compoumds were isolated from the n-BuOH portion of the 70% ethanolic extract of Comastoma pedunculatum by a combination of various chromatographic techniques including silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC, of which nine were triterpenoid saponins and four were flavone C-glycosides.
Abstract: Thirteen compoumds were isolated from the n-BuOH portion of the 70% ethanolic extract of Comastoma pedunculatum by a combination of various chromatographic techniques including silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC, of which nine were triterpenoid saponins and four were flavone C-glycosides. Their structures were elucidated by spectroscopic data as saikogenin F (1), 3-O-beta-D-fucopyranosylsaikogenin F (2), clinoposaponin XV (3), saikosaponin A (4), 6"-acetylsaikosaponin A (5), clinoposaponin I (6), bupleuroside I (7) , clinoposaponin XII (8) , saikoponin b3 (9), isovitexin (10) , swertisin (11) , isoorientin (12), 3',4',5-trihydroxy-7-methoxy-6-C-beta-D-glucopyranosyl-flavone (13). Compounds 1-10, 12-13 were all isolated from Comastoma genus for the first time.
Journal Article•
TL;DR: In this paper, the UPLC method was established for simultaneous determination of vitexin and isovitexins in mung bean and the separation was achieved on a C18 column with a mobile phase consisting of methanol-0.5% acetic acid (20:80).
Abstract: The UPLC method was established for simultaneous determination of vitexin and isovitexin in mung bean.The separation was achieved on a C18 column(2.1 mm×50 mm,1.7 μm) with a mobile phase consisting of methanol-0.5% acetic acid(20:80).The flow rate was 1 mL/min.The column temperature was maintained at 30 ℃.The detection wavelength was at 338 nm.The precision,stability and repeatability of the method were good.The linear range of vitexin and isovitexin was 0.685~6.85 μg/mL and 0.645~6.45 μg/mL,respectively.The average content of three batch samples was 0.0467% for vitexin and 0.0555% for isovitexin.The method was accurate and repeatable,which was better for quality evaluation of vitexin and isovitexin in mung bean.
Patent•
28 Mar 2012
Abstract: The invention discloses a method for extracting isovitexin from bamboo leave flavone. The method includes the steps as follows: (1) dissolving the bamboo leave flavone in normal propyl alcohol solution in volume percentage concentration of 20% and conducting filtration, thus obtaining crude isovitexin extract; and (2) and loading the crude isovitexin extract on a mixed resin column and conducting elution, separation, purification and recrystallization. The isovitexin extracted by the method is a natrual eaxtract, has high purity as well as antioxidation, anti-myocardial ischemia, anti-hypoxia, blood pressure lowering and inflammation diminishing effects and the like, and can be used for medicines, foods and health care products.