Showing papers on "Melibiose published in 1977"
TL;DR: It is demonstrated that the expected secondary amine linkages are formed with the ϵ-amino groups of lysine in synthetic glycoproteins prepared and compared with bovine serum albumin conjugates by amino acid analysis after acid hydrolysis.
219 citations
TL;DR: Affinity columns prepared by reductive amination of lactose, melibiose, maltose, and di-N-acetylchitobiose were used to purify the following lectins and have good flow rates, and high binding capacities.
123 citations
TL;DR: There was no correlation between the amount of residual receptor protein in these mutants and the initial rate of transport which ranged from 13 to 47% of the transport rate of the parent strain E. coli CR63.
48 citations
TL;DR: A gram-positive, rod-shaped bacterium, originally thought by Auger (1974) to be the agent of Pierce's disease of grapevines, is commonly associated with Hordnia circellata, one of the leafhopper vectors of the disease.
Abstract: A gram-positive, rod-shaped bacterium, originally thought by Auger (1974) to be the agent of Pierce's disease of grapevines, is commonly associated with Hordnia circellata, one of the leafhopper vectors of the disease. The bacterium has the following colonial and cellular characteristics: smooth, glistening white, or chalky colonies, 0.4 to 0.5 mm in diameter with entire margins; gram positive becoming gram variable after 5 days in culture; nonmotile and nonsporeforming; grows between 15 and 37°C with an optimum between 28 and 30°C and no growth at 9 or 45°C; optimum growth occurs at pH 6.5, with growth observed at pH 4.5 and pH 9.0. The bacterium does not survive after 5 to 7 days of growth and must be maintained on fresh medium. It is rod-shaped (0.6 by 1.5 to 2.0 μm), growing in short chains of three to four cells each, and possesses mesosomes and a cell wall of uniform thickness (28 to 35 nm) that consists of an electrondense outer layer and an inner layer resembling unit-membrane structure. The organism is a facultative anaerobe which reacts negatively in tests for cytochrome oxidase, catalase, “gelatinase,” urease, tryptophanase, and nitrate and disulfide reductase activities. No dextran or levan is produced from sucrose. It produces L-(+)-lactic acid but not D-(—)-lactic acid from glucose and sucrose fermentation, acetylmethylcarbinol, or arginine deaminase. Glucose, sucrose, galactose, maltose, fructose, trehalose, salicin, inulin, and cellobiose, but not gluconate, lactose, mannose, mannitol, sorbitol, melibiose, or raffinose, were utilized as carbon sources. Its chromosome has an average guanine-plus-cytosine content of 32.75 mol%. Based on these features, the bacterium appears to be a hitherto unrecognized species of the genus Lactobacillus, for which the name Lactobacillus hordniae sp. nov. is proposed. The type strain is HC-1 (= ATCC 29071).
33 citations
TL;DR: The invertases were different between all of the serotype but similar within the serotypes, and these findings support the taxonomic schemes of Coykendall and of Bratthall.
Abstract: Sucrase activity was studied in 13 strains of Streptococcus mutans representing the five Bratthall serotypes. Sucrose-adapted cells have sucrase activity in the 37,000 x g-soluble fraction of all strains. The enzyme was identified as invertase (beta-d-fructofuranoside fructohydrolase; EC 3.2.1.26) because it hydrolyzed the beta-fructofuranoside trisaccharide raffinose, giving fructose and melibiose as its products, and because it hydrolyzed the beta-fructofuranoside dissacharide sucrose, giving equimolar glucose and fructose as its products. Invertases of c and e strains exhibit two activity peaks by molecular exclusion chromatography with molecular weights of 45,000 to 50,000 and about 180,000; those of serotypes a, b, and d strains exhibit only a single component of 45,000 to 50,000 molecular weight. The electrophoretic mobility of invertases is different between the serotypes and the same within them. Inorganic orthophosphate (P(i)) has a weak positive effect on the V(max) of invertases of serotypes c and e cells but a strong positive effect on the invertases of serotype b cells; P(i) has a strong positive effect on the apparent K(m) of the invertases of serotype d cells, but has no effect on the V(max); P(i) has a strong positive effect on both the apparent K(m) and V(max) of the invertases of serotype a cells. Thus, the invertases were different between all of the serotypes but similar within the serotypes. These findings support the taxonomic schemes of Coykendall and of Bratthall. It was additionally noted that 37,000 x g-soluble fractions of only serotypes b and c but not serotypes a, d, and e cells have melibiase activity, and it could be deduced that serotype d cells lack an intact raffinose permease system.
31 citations
TL;DR: Ten of 13 selected substrates were hydrolyzed by crude extracts of midguts from larvae of Heliothis zea (Boddie) by thin-layer chromatography showed that a β-fructosidase occurs in the midgut.
Abstract: Ten of 13 selected substrates were hydrolyzed by crude extracts of midguts from larvae of Heliothis zea (Boddie). Sucrose was the most readily hydrolyzed; raffinose, melezitose, trehalose, glycogen, starch, melibiose, maltose, inulin, and α-methyl glucoside were less readily hydrolyzed. Cellulose, cellobiose, and lactose were not hydrolyzed. The p H optimum for each substrate was determined. Thin-layer chromatography showed that a β-fructosidase occurs in the midgut. Sucrase activity and p H of gut contents were determined each day for 5- to 11-day-old larvae.
13 citations
TL;DR: D-Aldohexopyranoside:cytochrome c oxidoreductase (ACO) was strongly induced by cellobiose, alpha-methylglucoside, beta- methylglucose, kojibiose, and sophorose; the active ACO inducers were poor glycosidase inducers; the converse also appeared to be true.
Abstract: D-Aldohexopyranoside:cytochrome c oxidoreductase (ACO) was strongly induced by cellobiose, alpha-methylglucoside, beta-methylglucoside, kojibiose, and sophorose. Induction was rapid, and ACO was readily detectable within 10 min after addition of cellobiose as inducer. Although not measurable for 30 to 40 min after addition of inducer, once started, the rate of induction with alpha-methylglucoside equaled or even exceeded that obtained with cellobiose. Induction by sucrose, maltose, alpha-alpha-trehalose, melibiose, and lactose was weak. In general, the active ACO inducers were poor glycosidase inducers; the converse also appeared to be true. Although ACO induction was not repressed by D-glucose, it was repressed by succinate, malate, and fumarate.
7 citations
TL;DR: Both preand postabsorption agglutination studies demonstrated the serological distinctiveness of Y.e.rh+ from typical Y. e.
Abstract: Thirteen atypical Yersinia enterocolitica isolates, all fermenting rhamnose, raffinose, and melibiose and utilizing sodium citrate within 24 to 48 h at 22 degrees C (Y.e.rh+), were examined biochemically-serologically, and by gas-liquid chromatography. These data, as well as cultural, biochemical, and antibiotic susceptibility data gathered from two previous studies involving (i) these same atypical Y.e.rh+ isolates, (ii) Y. enterocolitica serotypes O:1 through O:15 (rhamnose, raffinose, and citrate negative [Y.e.rh-]), (iii) Y. enterocolitica serotype O:16 (rhamnose positive but raffinose and citrate negative), and (iv) Yersinia pseudotuberculosis serogroups I through V were statistically compared. Both preand postabsorption agglutination studies demonstrated the serological distinctiveness of Y.e.rh+ from Y.e.rh- and Y. pseudotuberculosis. At the same time, three immunological groups among the 13 Y.e.rh+ strains were seen; 8 corresponded to Y. enterocolitica serotype O:17; 1 to Y. enterocolitica serotype O:16; and the remaining four were nontypable in antisera against known Y. enterocolitica antigen types. Each of the three Yersinia groups tested chromatographically produced acetic and lactic acids. Both Y.e.rh- and Y.e.rh+ formed propionic acid, but only Y.e.rh+ produced detectable amounts of succinic acid. Based on 49 variables, statistical analysis of the three Yersinia groups studied placed each of the Y.e.rh+ strains in a homogeneous group separate from both Y.e.rh- and Y. pseudotuberculosis. These data, coupled with deoxyribonucleic acid homology studies of Brenner and co-workers (D. J. Brenner, A. G. Steigerwalt, D. F. Falcao, R. E. Weaver, and G. R. Fanning, Int. J. Syst. Bacteriol. 26:180-194, 1976), support the distinctiveness of Y.e.rh+ from typical Y. enterocolitica and Y. pseudotuberculosis.
6 citations
TL;DR: Manninotriose undecaacetate has been prepared from melibiose as discussed by the authors, which was shown to give 6′-O-trityl-β-melibiose heptaacetate.
Abstract: Manninotriose undecaacetate has been prepared from melibiose. Tritylation of melibiose, followed by acetylation gave 6′-O-trityl-β-melibiose heptaacetate. Detritylation of the compound gave hepta-O-acetyl-β-melibiose, which was condensed with tetra-O-benzyl-D-galactopyranosyl chloride. Deacetylation of the condensation product, followed by hydrogenolysis and acetylation afforded the title compound.
4 citations
TL;DR: Present data suggest that VH/CN-71 may belong to the "flavus" series of Streptomyces, a streptomycete which can also produce acid from melibiose although it is unable to decompose xanthine.
Abstract: The organism VH/CN-71 which had been isolated from a growth in a cat and described initially as an "unusual strain of Nocardia" is a streptomycete. This organism can also produce acid from melibiose although it is unable to decompose xanthine. Its sporophores are short, hooked, curved, or open-looped with extended spirals. Present data suggest that VH/CN-71 may belong to the "flavus" series of Streptomyces.
2 citations
TL;DR: Manninotriose undecaacetate has been prepared from melibiose as discussed by the authors, which was shown to give 6′-O-trityl-β-melibiose heptaacetate.
Abstract: Manninotriose undecaacetate has been prepared from melibiose. Tritylation of melibiose, followed by acetylation gave 6′-O-trityl-β-melibiose heptaacetate. Detritylation of the compound gave hepta-O-acetyl-β-melibiose, which was condensed with tetra-O-benzyl-D-galactopyranosyl chloride. Deacetylation of the condensation product, followed by hydrogenolysis and acetylation afforded the title compound.