Showing papers on "Nucleic acid methods published in 1988"

Nucleic acid intercalating agents

Abstract: Pyridinone or pyrimidinone nucleoside bases containing fused aromatic polycyclic rings are provided. These polycyclic nucleosides are incorporated into oligonucleotides and hybridized to complementary nucleic acid. Fluorescence spectroscopy and thermal denaturation profiles provided evidence that the polycyclic base is intercalated into the resulting duplex. The fused polycyclic ring systems optionally are substituted with reactive species which inactivate complementary nucleic acids. The oligonucleotides of this invention are useful as improved probes, diagnostic reagents, or for cleaving or derivatizing predetermined domains within nucleic acids.

Nucleic acid multimers and amplified nucleic acid hybridization assays using same

Abstract: Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.

Transcription-based nucleic acid amplification/detection systems

Abstract: The present invention is predicated on the novelty of certain RNA transcripts, their production, optional replication, and use, to achieve desired amplification and detection of corresponding (in sequence) target nucleic acid sequence. The transcripts correspond in sequence to a target nucleic acid sequence contained in an original sample amongst a mixture of nucleic acids, and therefore, the presence of the transcripts in amplified form provides for their detection, and hence by correspondence, the in vitro or x-vivo detection of the presence of the target nucleic acid sequence in said sample.

Means and method for enhancing nucleic acid hybridization

Abstract: The binding of a nucleic acid probe with its complementary sequence in a targeted, single stranded nucleic acid is affected by the secondary and tertiary structure of the target nucleic acid. The rate and extent of hybridization of the probe with the targeted nucleic acid can be increased by the use of "helper" oligonucleotides. Helper oligonucleotides are selected to bind to the target nucleic acid and impose a different secondary and tertiary structure on the target to facilitate the binding of the probe to the target. The resulting hybrid of probe and target nucleic acid also exhibits a higher T m than the hybrid which results from addition of the probe alone.

Polycationic supports for nucleic acid purification, separation and hybridization

Abstract: Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without significant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure.

Assay for nucleic acid sequences in a sample

Abstract: A method for detecting (i) one or more microorganisms or (ii) nucleic acid sequences from a prokaryotic source or an eukaryotic source in a nucleic acid-containing test sample comprising (a) labeling the nucleic acids in the test sample, (b) contacting, under hybridization conditions, the labeled hybridizable nucleic acid and one or more immobilized hybridizable nucleic acid probes comprising (i) one or more known microorganisms or (ii) sequences from eukaroytic or prokaryotic sources, to form hybridized labeled nucleic acids, and (d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia.

Nucleic acid probes of human papilloma virus

Abstract: The present invention relates to probes of nucleic acids useful for detecting indifferently the various types of human papilloma virus, particularly HPV1a, HPV5, HPV6b, HPV8, HPV11, HPV16, HPV18 and HPV33, especially a probe comprising a labelled sequence of nucleic acids, characterized in that it comprises the oligomer of twelve nucleotides X-A-A-A-A-C-G-A-A-A-G-X, with X=T or U, or its complement by interchanging A and X on the one hand, C and G on the other hand. The present invention also relates to specific probes of nucleic acids for the detection of human papilloma for each of the types HPV1a, HPV5, HPV8, HPV11, HPV16, HPV18 and HPV33, as well as specific probes of sub-groups of the virus HPV16, HPV18, HPV33 or HPV16 and HPV18 only or again HPV5 and HPV8 only.

Method for sequencing nucleic acids

Abstract: In order to sequence nucleic acids one produces a mixture of labelled nucleic acid fragments of different length, using nucleic acid fragments which are labelled by incorporation of at least one deoxyribonucleoside triphosphate with a non-radioactive labelling group, separates the labelled nucleic acid fragments according to size and determines the nucleic acid sequence by means of the labelling of the individual fragments.

Process for isolating nucleic acids

Abstract: A process for the isolation of nucleic acids from cell or tissue extracts by precipitating the nucleic acids with a water-soluble ketone, preferably with acetone, optionally in the presence of dimethylformamide or formamide.

Nucleic acid detection with separation

Abstract: Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nucleic acid from free and non-specifically immobilized nucleic acid, releasing specifically immobilized nucleic acid, and detecting the presence of the sequence of interest by means of the label. The labeled sequence may be characterized by sizing, e.g. electrophoresis. The method provides for a sensitive and rapid means for accurate detection of sequences of interest in a wide variety of situations.

Method for detecting target nucleic acid in specimen

Abstract: A method for detecting a target nucleic acid in a specimen, which comprises hybridizing in a specimen a primer of at least one strand of the nucleic acid chains of a target nucleic acid and adding a unit nucleic acid thereto for elongation of the chain to thereby prepare a synthetic nucleic acid complementary to and hybridized with said strand, thus obtaining a copy of the target nucleic acid comprising (a) the double-stranded nucleic acid thus formed, (b) a synthetic nucleic acid prepared by separating the double-stranded nucleic acid into a single-stranded nucleic acid, or (c) a double-stranded nucleic acid which is a double-stranded hybridized product complementary to the synthetic nucleic acid, and examining whether this copy exists or not in the sample.

Method and kit for detecting a nucleic acid sequence

Abstract: Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nucleic acid from free and non-specifically immobilized nucleic acid, releasing specifically immobilized nucleic acid, and detecting the presence of the sequence of interest by means of the label. The labeled sequence may be characterized by sizing, e.g. electrophoresis. The method provide for a sensitive and rapid means for accurate detection of sequence of interest in a wide variety of situations.

HPLC in nucleic acids research.

Abstract: The separation capabilities of ion exchange, when coupled with the principles of reversed-phase fractionation, yields an advanced technology for the manipulation of nucleic acids.

Nucleic acid capture reagent

Abstract: A nucleic acid capture reagent which comprises one or more molecules that are capable of intercalation into nucleic acids and are attached to a solid support via a molecular linker. This capture reagent is useful for the separation and isolation of nucleic acids from complex unpurified biological solutions such as serum, sputum, blood, and urine. The resulting capture reagent-nucleic acid complexes are easily isolated from the sample solution by mechanical or magnetic means and the bound nucleic acids are released by simple chemical treatment.

Process and means for the determination of nucleic acids

Abstract: Die Erfindung betrifft Verfahren und Mittel zur Bestimmung von Nucleinsauren in einer Probe durch Uberfuhrung der zu bestimmenden Nucleinsauresequenzen in Einzelstrange und Umsetzung der Einzelstrange mit einer komplementaren Poly nucleotidsonde. In der Polynucleotidsonde ist Cytidin durch 5-Aza-2′-desoxy-cytidin oder 5-Aza-cytidin ersetzt. Die Sonde wird nach Bindung mit gegebenenfalls markierter DNA-Methylase und Nachweise der Methylase uber spezifische, gegebenenfalls markierte anti-Methylase-Antikorper oder uber den Marker bestimmt.

A Polymeric Support for Chemical and Enzymatic Nucleic Acid Synthesis (1)

Abstract: A non-swellable, highly porous support material -CPG 3000 - was used in building up covalently bound nucleic acids by combined chemical and enzymatic methods. Bases are optimal accessible for hybridization and enzymatic reactions because they are not involved in the linking procedure.

Method of detecting single base substitutions in nucleic acids

Abstract: A method for detecting point mutations or base substitutions in a nucleic acid polymer is especially useful for detecting such mutations in the highest melting domain (HMD) of a double-stranded nucleic acid polymer and is particularly suited for use with RNA. The method involves the steps of: (a) preparing a solution containing a double-stranded nucleic acid polymer comprising a duplex of a single-stranded nucleic acid polymer to be analyzed and a c The invention described and claimed in this application was made with government support under Grant #DK-38381 awarded by the National Institutes of Health. The U.S. government has certain rights in the invention.

Process for the preparation of nucleic acids

Abstract: Process for the isolation of nucleic acids from cell or tissue extracts, characterised in that the nucleic acids are precipitated with water-soluble ketone, preferably with acetone, if appropriate in the presence of dimethylformamide or formamide.

Detection of aimed nucleic acid in specimen

Abstract: PURPOSE: To make possible to readily detect in high sensitivity, by subjecting a primer complimentary to a nucleic acid sequence to be detected to an elongation reaction, fixing the nucleic acid sequence of resultant elongated substance and detecting. CONSTITUTION: At least one primer complimentary to a nucleic acid sequence to be detected is used. The primer complimentary to the nucleic acid sequence is subjected to elongation reaction and the resultant nucleic acid sequence, or other nucleic acid sequence part having two-chains formed with individual elongated primer substances are detected after fixed to a solid phase carrier. COPYRIGHT: (C)1989,JPO&Japio

Assay of nucleic acid sequence in nucleic acid-containing specimen

Abstract: A method for detecting (i) one or more microorganisms or (ii) nucleic acid sequences from a prokaryotic source or an eukaryotic source in a nucleic acid-containing test sample comprising (a) labeling the nucleic acids in the test sample, (b) contacting, under hybridization conditions, the labeled hybridizable nucleic acid and one or more immobilized hybridizable nucleic acid probes comprising (i) one or more known microorganisms or (ii) sequences from eukaroytic or prokaryotic sources, to form hybridized labeled nucleic acids, and (d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia. o

A rapid method for identifying a specific nucleic acid sequence

Abstract: The present method provides a nucleic acid hybridization method for identifying a specific nucleic acid sequence, especially from a microorganism in a culture thereof. The method utilizes volumes of about 5-30 microliters of a highly concentrated nucleic acid probe reagent, the low volumes being applied to a limited area of about 2-5 millimeters comprising an isolated nucleic acid binding surface.

Polycationic supports for nucleic acid purification separation and hybridization

Abstract: @ Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without signficant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure. o