Showing papers on "Rapeseed published in 2023"

Biodiesel from Rapeseed and Sunflower Oil: Effect of the Transesterification Conditions and Oxidation Stability

Abstract: In this study, we produced biodiesel fuel from two vegetal sources, rapeseed oil and sunflower oil, by transesterification reaction. The study aims to evaluate the impact of type of alcohol, its concentration and the reaction time, while keeping constant the temperature and the catalyst on the yield and quality of the biodiesel. For alcohol, methanol and ethanol were used at a molar ratio with the oil from 3 to 24. Transesterification was performed at various reaction times; 20, 40, 60 and 90 min for each oil and defined alcohol:oil molar ratio. The influence of these parameters on the biodiesel yield and properties were investigated in terms of density, viscosity, heating value, flash point, elemental content, density and oxidative stability of the final product. The benefit of oxidation stabilizers, catechol and 4-allyl-2,6-dimethoxyphenol was investigated. Results demonstrate that for rapeseed oil, the optimum reaction conditions to obtain a higher yield and quality of biodiesel were an alcohol:oil molar ratio of 15:1, with 60-min reaction time at 50 °C; while in the case of sunflower oil, the best yield and biodiesel quality were at an 18:1 molar ratio, with a 40-min reaction time and at 50 °C. In both cases, methanol provides the highest yields of biodiesel, and the obtained products satisfy the required standards and present a similarity with mineral diesel tested in same conditions.

Abscisic acid modulates differential physiological and biochemical responses to cadmium stress in Brassica napus

Abstract: The plant hormone abscisic acid (ABA) has been proved to be a key protector against abiotic stresses at appropriate concentrations. This study aimed to investigate the role of ABA in alleviating cadmium (Cd) stress in two Brassica napus of oil-vegetable dual-purpose (Youfei 1 and Xiangyou 787). Cd stress disturbed the normal growth of rapeseed, however, specific ABA concentrations could alleviate the effects of Cd stress in B. napus. The results confirmed that exogenous ABA mitigates the negative effects of Cd-induced plant toxicity in B. napus by increasing growth traits, enhancing pigment molecules, enhancing gas exchange in leaves, as well as regulating antioxidation and the expression levels of genes related to Cd stress. This study indicated that ABA played an important protective role in regulating cadmium resistance in Brassica napus, and provided basic data for its application in actual production.

Effects of working parameters on the performance of cyclone separator for rapeseed combine harvester based on CFD

Abstract: Existing development for cyclone separation cleaning components of the rapeseed combine harvester, which employs the suspending airflow to separate the rapeseeds from the materials other than grain (MOG), has the challenge to figure out the optimal working parameters, highlighting a need for exploration of the invisible airflow based on Computational Fluid Dynamics (CFD). The airflow status was mainly affected by the air velocities of the inlet, and the outlet for the MOG. The single factor and response surface experiments were carried out. It could be found that the inlet and MOG outlet velocities affected the air velocities through the change in the air quantity. Furthermore, the mathematical model of the relationship between the air velocities inside the cyclone and the working parameters was built, and the optimal combination of working parameters was obtained by multi-objective optimization. The inlet and outlet velocities of the optimal combination were 4.25 m/s and 29.87 m/s, respectively. Under this condition, the cleaning ratio and loss ratio of the cleaning device was 94.62% and 5.39%, respectively, as validated by the field experiment. The findings provide references for the improvement of cleaning systems for rapeseed combine harvesters. Keywords: rapeseed, combine harvester, cyclone separation cleaning, optimal working parameters, CFD DOI: 10.25165/j.ijabe.20231601.7253 Citation: Wan X Y, Yuan J C, Yang J, Liao Y T, Liao Q X. Effects of working parameters on the performance of the cyclone separator for rapeseed combine harvester based on CFD. Int J Agric & Biol Eng, 2023; 16(1): 128–135.

Determination of glucosinolates and isothiocyanates in glucosinolate-rich vegetables and oilseeds using infrared spectroscopy: A systematic review.

Abstract: Cruciferous vegetables and oilseeds are rich in glucosinolates that can transform into isothiocyanates upon enzymic hydrolysis during post-harvest handling, food preparation and/or digestion. Vegetables contain glucosinolates that have beneficial bioactivities, while glucosinolates in oilseeds might have anti-nutritional properties. It is therefore important to monitor and assess glucosinolates and isothiocyanates content through the food value chain as well as for optimized crop production. Vibrational spectroscopy methods, such as infrared (IR) spectroscopy, are used as a nondestructive, rapid and low-cost alternative to the current and common costly, destructive, and time-consuming techniques. This systematic review discusses and evaluates the recent literature available on the use of IR spectroscopy to determine glucosinolates and isothiocyanates in vegetables and oilseeds. NIR spectroscopy was used to predict glucosinolates in broccoli, kale, rocket, cabbage, Brussels sprouts, brown mustard, rapeseed, pennycress, and a combination of Brassicaceae family seeds. Only one study reported the use of NIR spectroscopy to predict broccoli isothiocyanates. The major limitations of these studies were the absence of the critical evaluation of errors associated with the reference method used to develop the calibration models and the lack of interpretation of loadings or regression coefficients used to predict glucosinolates.

Resynthesized Rapeseed (Brassica napus): Breeding and Genomics

Abstract: Abstract Resynthesized rapeseed lines (2n = 4x = 38, AACC), which recreate the historical hybridization between progenitor species Brassica rapa (2n = 2x = 20, AA) and B. oleracea (2n = 2x = 18, CC) to produce Brassica napus (2n = 4x = 38, AACC), have been an important research subject for many years. These lines not only comprise useful genetic resources in rapeseed breeding for the introgression of genetic diversity and many agronomically important traits, but have also increased our understanding of how meiosis evolved in polyploid plants. In this review, we discuss and summarize how these lines have been produced via interspecific crosses over several decades and the resulting fertility and agronomically useful traits in this germplasm, as well as meiotic instability issues in these hybrids, and the putative role of meiosis genes, gene expression and other gene regulatory networks in the stabilization of meiosis. Finally, we discuss the direct use of resynthesized and semi-resynthesized Brassica napus for hybrid breeding as well as for the study of other unexplored agronomic traits of interest for the improvement of elite rapeseed cultivars.

Functional Analysis of Bna-miR399c-PHO2 Regulatory Module Involved in Phosphorus Stress in Brassica napus

Abstract: Phosphorus stress is one of the important factors restricting plant growth and development, and the microRNA (miRNA) family is involved in the regulation of the response to plant nutrient stress by repressing the expression of target genes at the post-transcriptional or translational level. miR399 is involved in the transportation of phosphate in multiple plants by improving tolerance to low Pi conditions. However, the effect of miR399 on the response of low Pi stress in rapeseed (Brassica napus L.) is unclear. The present study showed a significant increase in taproot length and lateral root number of plants overexpressing Bna-miR399c, while the biomass and Pi accumulation in shoots and roots increased, and the anthocyanin content decreased and chlorophyll content improved under low Pi stress. The results illustrate that Bna-miR399c could enhance the uptake and transportation of Pi in soil, thus making B. napus more tolerant to low Pi stress. Furthermore, we confirmed that BnPHO2 is one of the targets of Bna-miR399c, and the rejection of Pi in rapeseed seedlings increased due to the overexpression of BnPHO2. Hence, we suggest that miR399c-PHO2 module can effectively regulate the homeostasis of Pi in B. napus. Our study can also provide the theoretical basis for germplasm innovation and the design of intelligent crops with low nutrient input and high yield to achieve the dual objectives of income and yield increase and environmental protection in B. napus.

Cold-Pressed Oilseed Cakes as Alternative and Sustainable Feed Ingredients: A Review

Abstract: Due to the increasing demand for alternative protein feed ingredients, the utilization of oilseed by-products in animal nutrition has been sought as a promising solution to ensure cheap and environmentally sustainable feedstuffs. This review aimed to summarize the nutritional value of six cold-pressed cakes (rapeseed, hempseed, linseed, sunflower seed, camelina seed, and pumpkin seed) and the effects of their inclusion in diet for ruminant, pig, and poultry on nutrient digestibility, growth and productive performance, and quality of the products. The presented results indicated that these unconventional feed ingredients are a good protein and lipid source and have a balanced amino acid and fatty acid profile. However, contradictory results of animal production performances can be found in the literature depending on the cake type and chemical composition, dietary inclusion level, animal category, and trial duration. Due to the substantial amount of essential fatty acid, these cakes can be efficiently used in the production of animal products rich in n-3 and n-6 polyunsaturated fatty acids. However, the utilization of cakes in pig and poultry nutrition is limited because of the presence of antinutritive factors that can deteriorate feed intake and nutrient utilization.

Effect of the Bleaching Process on Changes in the Fatty Acid Profile of Raw Hemp Seed Oil (Cannabis sativa)

Abstract: Many refined oils from soybean, rapeseed, and sunflower, among others, are available on the food market, except olive oil. Refining, on the small production scale of niche oils, is not used due to the high cost of the refining process. Unrefined oils are characterized by intense taste, odor, color, and undesirable nutrients. The problem to be solved is determining the effects of incomplete refining of niche oils on their composition. One process, which does not require the use of complex apparatus, is the bleaching process. The results presented in this article relate to the research stage, in which the aim is to evaluate the changes occurring in the oil due to the low-temperature bleaching process with different process parameters. The presented research results provide evidence of the absence of adverse changes in the fatty acid profile of hemp oil of the varieties ‘Finola’, ‘Earlina 8FC’, and ‘Secuieni Jubileu’. Seven different types of bleaching earth were used to bleach the oil in amounts of 2.5 and 5 g/100 g of vegetable oil. The fatty acid profile was obtained by gas chromatography (GC-FID). The obtained chromatograms were subjected to statistical analysis and principal component analysis (PCA). The results show that there was no effect of the type of bleaching earth and its amount on the change in the fatty acid profile of bleached oils. Only real differences between the types of hemp oils were observed. However, an overall positive effect of the bleaching process on hemp oil was found. The amount of saturated fatty acid (SFA) was reduced by 17.1% compared with the initial value, resulting in an increase in the proportion of polyunsaturated fatty acids (PUFA) by 4.4%, resulting in an unsaturated fatty acid (UFA) proportion of 90%. There was a significant improvement in the SFA/PUFA ratio by 26% over the baseline, and the omega-6/omega-3 ratio by 8.9% to a value of 3.1:1. The new knowledge from this study is evidence of the positive effect of the low-temperature bleaching process on the fatty acid profile. In contrast, the parameters of the bleaching process itself are not significant.

Monitoring of Performance-based Environmental Impacts of Substituting Soybean Meal with Rapeseed Meal in the Rye-based Diet of Weaned Pigs

Abstract: Due to its favorable properties, soybean meal (SBM) is used especially in young growing animals. In terms of sustainability, there are various efforts to reduce the amounts of SBM in compound feeds and to increase the use of regional protein sources. This paper focuses on the effects of a partial to total substitution of SBM by regionally produced rapeseed meal (RSM) in different piglet diets regarding 10 important factors having an impact on the environment. Four diets, characterized by different shares (%) of both protein-rich ingredients (SBM/RSM: 18.1/0; 13.6/6.70; 8.10/16.1; 0/28.0), were fed to four groups of 10 piglets each in two runs. The impact was calculated related to feed (per t) and was performance-based for every piglet (impact·kg weight gain−1) for each factor using methods according to life-cycle-analyses (LCA). Although feed intake and weight gains were not affected negatively, higher feed conversion ratios occurred, with high amounts of rapeseed inclusion. Nevertheless, the performance-based negative influence on climate change (kg CO2 eq·kg weight gain−1) was nearly halved when SBM was replaced by RSM. Since performance was not negatively affected, the use of RSM instead of SBM in piglet diets could be a viable tool for markedly reducing the negative impact on climate change.

Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes

Abstract: Abstract The presence of anti-nutritive compounds like glucosinolates (GSLs) in the rapeseed meal severely restricts its utilization as animal feed. Therefore, reducing the GSL content to < 18 µmol/g dry weight in the seeds is a major breeding target. While candidate genes involved in the biosynthesis of GSLs have been described in rapeseed, comprehensive functional analyses are missing. By knocking out the aliphatic GSL biosynthesis genes BnMYB28 and BnCYP79F1 encoding an R2R3 MYB transcription factor and a cytochrome P450 enzyme, respectively, we aimed to reduce the seed GSL content in rapeseed. After expression analyses on single paralogs, we used an ethyl methanesulfonate (EMS) treated population of the inbred winter rapeseed ‘Express617’ to detect functional mutations in the two gene families. Our results provide the first functional analysis by knock-out for the two GSL biosynthesis genes in winter rapeseed. We demonstrate that independent knock-out mutants of the two genes possessed significantly reduced seed aliphatic GSLs, primarily progoitrin. Compared to the wildtype Express617 control plants (36.3 µmol/g DW), progoitrin levels were decreased by 55.3% and 32.4% in functional mutants of BnMYB28 (16.20 µmol/g DW) and BnCYP79F1 (24.5 µmol/g DW), respectively. Our study provides a strong basis for breeding rapeseed with improved meal quality in the future.

Tissue-Specific Transcriptome and Metabolome Analysis Reveals the Response Mechanism of Brassica napus to Waterlogging Stress

Abstract: During the growth period of rapeseed, if there is continuous rainfall, it will easily lead to waterlogging stress, which will seriously affect the growth of rapeseed. Currently, the mechanisms of rapeseed resistance to waterlogging stress are largely unknown. In this study, the rapeseed (Brassica napus) inbred lines G230 and G218 were identified as waterlogging-tolerant rapeseed and waterlogging-sensitive rapeseed, respectively, through a potted waterlogging stress simulation and field waterlogging stress experiments. After six days of waterlogging stress at the seedling stage, the degree of leaf aging and root damage of the waterlogging-tolerant rapeseed G230 were lower than those of the waterlogging-sensitive rapeseed G218. A physiological analysis showed that waterlogging stress significantly increased the contents of malondialdehyde, soluble sugar, and hydrogen peroxide in rape leaves and roots. The transcriptomic and metabolomic analysis showed that the differential genes and the differential metabolites of waterlogging-tolerant rapeseed G230 were mainly enriched in the metabolic pathways, biosynthesis of secondary metabolites, flavonoid biosynthesis, and vitamin B6 metabolism. Compared to G218, the expression levels of some genes associated with flavonoid biosynthesis and vitamin B metabolism were higher in G230, such as CHI, DRF, LDOX, PDX1.1, and PDX2. Furthermore, some metabolites involved in flavonoid biosynthesis and vitamin B6 metabolism, such as naringenin and epiafzelechin, were significantly up-regulated in leaves of G230, while pyridoxine phosphate was only significantly down-regulated in roots and leaves of G218. Furthermore, foliar spraying of vitamin B6 can effectively improve the tolerance to waterlogging of G218 in the short term. These results indicate that flavonoid biosynthesis and vitamin B6 metabolism pathways play a key role in the waterlogging tolerance and hypoxia stress resistance of Brassica napus and provide new insights for improving the waterlogging tolerance and cultivating waterlogging-tolerant rapeseed varieties.

Discovery of common loci and candidate genes for controlling salt-alkali tolerance and yield-related traits in Brassica napus L.

Abstract: Common loci and candidate genes for controlling salt-alkali tolerance and yield-related traits were identified in Brassica napus combining QTL mapping with transcriptome under salt and alkaline stresses. The yield of rapeseed (Brassica napus L.) is determined by multiple yield-related traits, which are susceptible to environmental factors. Many yield-related quantitative trait loci (QTLs) have been reported in Brassica napus; however, no studies have been conducted to investigate both salt-alkali tolerance and yield-related traits simultaneously. Here, specific-locus amplified fragment sequencing (SLAF-seq) technologies were utilized to map the QTLs for salt-alkali tolerance and yield-related traits. A total of 65 QTLs were identified, including 30 QTLs for salt-alkali tolerance traits and 35 QTLs for yield-related traits, accounting for 7.61-27.84% of the total phenotypic variations. Among these QTLs, 18 unique QTLs controlling two to four traits were identified by meta-analysis. Six novel and unique QTLs were detected for salt-alkali tolerance traits. By comparing these unique QTLs for salt-alkali tolerance traits with those previously reported QTLs for yield-related traits, seven co-localized chromosomal regions were identified on A09 and A10. Combining QTL mapping with transcriptome of two parents under salt and alkaline stresses, thirteen genes were identified as the candidates controlling both salt-alkali tolerance and yield. These findings provide useful information for future breeding of high-yield cultivars resistant to alkaline and salt stresses.

Performance analysis of rapeseed-mustard crop under different agro-climatic conditions of Jammu Division of J & K state

Abstract: Rapeseed-mustard is the second most important edible oilseed crop in India after groundnut. It has to play a significant role in making India self-reliant in oilseed sector. At present contribution of India to the world acreage and production is 19.29% and 11.12% respectively. Productivity of rapeseed mustard in J & K is 6.98 q/ha which is 37.72% of global productivity and 67.85% of national productivity. Keeping in view low productivity of rapeseed mustard crop in J & K state, a study was undertaken with randomly selected 200 rapeseed mustard growers 100 each from subtropical and temperate zone of Jammu division covering Jammu, Samba, Reasi and Doda districts to ascertain the performance of rapeseed mustard crop under farmer field conditions. Multiple linear regression model was applied to work out the factors affecting productivity of rapeseed mustard crop. The results revealed that proper irrigation, spray of insecticide, proper sowing method, thinning, use of basal dose of urea at sowing time and proper weed management significantly affected the productivity of Indian mustard (Brassica juncea) with R2 value 0.534. The drivers of productivity of gobhi sarson (Brassica napus) were proper time of sowing and irrigation with R2 value 0.253 while use of phosphorus and irrigation at proper time were found to be significantly affecting the productivity of toria (Brassica rapa) with R2 value 0.757. The factors affecting the productivity of hybrid gobhi sarson were proper weed control and knowledge about different production recommendations with R2 value 0.080.

Comparative proteomic and physiological analyses reveal tribenuron-methyl phytotoxicity and nontarget-site resistance mechanisms in Brassica napus.

Abstract: The application of herbicides is the most effective strategy for weed control and the development of herbicide-resistant crops will facilitate the weed management. The acetolactate synthase-inhibiting herbicide, tribenuron-methyl (TBM), is broadly used for weed control. However, its application in rapeseed field is restricted since rapeseed is sensitive to TBM. Herein, an integrated study of cytological, physiological and proteomic analysis of the TBM-resistant rapeseed mutant M342 and its wild-type (WT) plants was conducted. After TBM spraying, M342 showed improved tolerance to TBM, and proteins implicated in non-target-site resistance (NTSR) to herbicides had a significantly higher level in M342 as compared with the WT. Differentially accumulated proteins (DAPs) between these two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, which protected the mutant from oxidative stress triggered by TBM. Important DAPs related to stress or defence response were up-accumulated in M342 regardless of the TBM treatment, which might serve as the constitutive part of NTSR to TBM. These results provide new clues for further exploration of the NTSR mechanism in plants and establish a theoretical basis for the development of herbicide-resistant crops.

Pyrus glabra seed oil as a new source of mono and polyunsaturated fatty acids: Composition, thermal, and FTIR spectroscopic characterization

Abstract: Edible oils are used worldwide, but there is a shortage in developing countries, especially those that do not produce olive, soy, and rapeseed oils. Trying to explore the Iranian biodiversity, Pyrus glabra seeds were studied for the extraction and characterization of the oil. Seven fatty acids were presented in P. glabra seed oil, with oleic acid (ω-9) accounting for 52.1% of the total oil, followed by linoleic acid, ω-6 (33.75%) and palmitic acid (6.69%). P. glabra seed oil had a refractive index of 1.47 at room temperature and contained 5.5 mg KOH/g, 69.4 g I2/100 g, and 134.3 mg KOH/g in terms of acid value, iodine value, and saponification value. FTIR of P. glabra seed oil showed peaks related to several functional groups, including hydrogen bonds, double bonds, and CO, C–O, and C–C bonds. The DSC analysis of the seed oil revealed the detection of three exothermal peaks during cooling, which corresponded to different crystallization temperatures of the fatty acid components in the oil. The most significant endothermal and exothermal peaks were observed at low temperatures during the melting process. It is potential for P. glabra seed oil to be fully developed and utilized with the help of this research.

Flavonoid Synthesis-Related Genes Determine the Color of Flower Petals in Brassica napus L.

Abstract: The color of rapeseed (Brassica napus L.) petal is usually yellow but can be milky-white to orange or pink. Thus, the petal color is a popular target in rapeseed breeding programs. In his study, metabolites and RNA were extracted from the yellow (Y), yellow/purple (YP), light purple (LP), and purple (P) rapeseed petals. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), RNA-Seq, and quantitative real-time (qRT-PCR) analyses were performed to analyze the expression correlation of differential metabolites and differential genes. A total of 223 metabolites were identified in the petals of the three purple and yellow rapeseed varieties by UPLC-MS/MS. A total of 20511 differentially expressed genes (DEGs) between P, LP, YP, versus Y plant petals were detected. This study focused on the co-regulation of 4898 differential genes in the three comparison groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation and quantitative RT-PCR analysis showed that the expression of BnaA10g23330D (BnF3'H) affects the synthesis of downstream peonidin and delphinidin and is a key gene regulating the purple color of petals in B. napus. L. The gene may play a key role in regulating rapeseed flower color; however, further studies are needed to verify this. These results deepen our understanding of the molecular mechanisms underlying petal color and provide the theoretical and practical basis for flower breeding targeting petal color.

Study on the hypolipidemic activity of rapeseed protein-derived peptides.

Abstract: Hyperlipidaemia, a common chronic disease, is the cause of cardiovascular diseases such as myocardial infarction and atherosclerosis. Generally, drugs for lowering blood lipids have disadvantages such as short or poor efficacy, high toxicity, and side effects. Rapeseed active peptides are excellent substitutes for lipid-lowering drugs because of their high biological safety, strong penetration, and easy absorption by the human body. This study separated and purified the rapeseed peptides using gel chromatography and mass spectrometry. Rapeseed peptides amino acid sequences were determined to obtain Glu-Phe-Leu-Glu-Leu-Leu (EFLELL) peptides with good hypolipidaemic activity and IC50 values of 0.1973 ± 0.05 mM (sodium taurocholate), 0.375 ± 0.03 mM (sodium cholate), and 0.203 ± 0.06 mM (sodium glycine cholate). The EFLELL hypolipidaemic activity was evaluated, and its mechanism of action was investigated using cell lines. Rapeseed peptide treatment significantly decreased the total cholesterol (T-CHO), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels, and the protein and gene expression levels of proprotein convertase subtilisin/kexin type 9 (PCSK9) and low-density lipoprotein cholesterol (LDLR) suggested the mechanism. Molecular docking revealed that the binding energy between rapeseed peptide and LDLR-PCSK9 molecules was -6.3 kcal/mol and -8.1 kcal/mol. In conclusion, the rapeseed peptide EFLELL exerts a favourable hypolipidaemic effect by modulating the LDLR-PCSK9 signalling pathway.

Role of tillage measures in mitigating waterlogging damage in rapeseed

Abstract: Tillage measures have been effectively adopted for mitigating waterlogging damage in field crops, yet little is known about the role of tillage measures in crop responses to waterlogging. A field experiment was performed to investigate the effect of conventional planting (CK), small ridge planting (SR), big ridge planting (BR) and film side planting (FS) on soil available nutrients and enzymatic activity, chlorophyll contents, leaf nutrients, soluble protein, soluble sugar, nitrate reductase, antioxidant enzyme activity, lipid peroxidation, agronomic traits and yield of rapeseed under waterlogging stress conditions.Tillage measures remarkably improved rapeseed growth and yield parameters under waterlogging stress conditions. Under waterlogging conditions, rapeseed yield was significantly increased by 33.09 and 22.70% in the SR and BR groups, respectively, compared with CK. Correlation analysis showed that NO3--N, NH4+-N, and urease in soils and malonaldehyde (MDA), superoxide dismutase (SOD), and nitrate reductase in roots were the key factors affecting rapeseed yield. The SR and BR groups had significantly increased NO3--N by 180.30 and 139.77%, NH4+-N by 115.78 and 66.59%, urease by 41.27 and 26.45%, SOD by 6.64 and 4.66%, nitrate reductase by 71.67 and 26.67%, and significantly decreased MDA content by 14.81 and 13.35% under waterlogging stress, respectively, compared with CK. In addition, chlorophyll and N content in leaves, soluble sugar and POD in roots, and most agronomic traits were also significantly enhanced in response to SR and BR under waterlogging conditions.Overall, SR and BR mitigated the waterlogging damage in rapeseed mainly by reducing the loss of soil available nitrogen, decreasing the MDA content in roots, and promoting urease in soils and SOD and nitrate reductase in roots. Finally, thorough assessment of rapeseed parameters indicated that SR treatment was most effective followed by BR treatment, to alleviate the adverse effects of waterlogging stress.

Effect of Fermented Rapeseed Meal in Feeds for Growing Piglets on Bone Morphological Traits, Mechanical Properties, and Bone Metabolism

Abstract: Simple Summary Strong and healthy bones allow for efficient locomotion and overall functioning of the musculoskeletal system of pigs. This is the first study to provide information on the effect of including fermented rapeseed meal as a partial wheat replacement in the diet on bone quality in weaner piglets. Abstract Quality feed is essential for correct bone development and proper functioning of animals. Post-weaned piglets experience a radical change in eating behaviour that can influence their feed intake. For this reason, functional feed additives and ingredients that can be used in post-weaning feeds are needed. The objective of this study was to evaluate the effects of partially replacing wheat with rapeseed meal fermented using Bacillus subtilis strain 87Y on overall bone quality and bone metabolism in weaner piglets. From the 28th day of life, barrows were fed either a standard wheat-based diet or a diet containing 8% fermented rapeseed meal (FRSM) with or without a feed additive containing enzymes, antioxidants, probiotics, and prebiotics. The experimental period lasted 60 days, after which femur quality indices were assessed. Differences in bone length and weight were observed, but there were no changes in bone mineralization or bone mid-diaphysis morphometrical traits between treatments. FRSM inclusion reduced bone mid-diaphysis biomechanical properties, but these changes were dependent on feed-additive supplementation. Analysis of the levels of serum bone turnover markers suggests the intensification of bone resorption in FRSM-fed groups as deoxypyridinoline levels increase. The results obtained warrant further research on what the disturbances in bone mechanical properties and metabolism observed in FRSM-fed weaners means for the subsequent fattening period.