Showing papers on "Swertia published in 2009"

Molecular profiling of "Chirayat" complex using Inter Simple Sequence Repeat (ISSR) markers.

Abstract: “Chirayat” represents a group in which different species of SWERTIA and some other non- SWERTIA species are used as local substitutes/adulterants for the SWERTIA CHIRAYITA. Twelve different taxa recognized under “Chirayat” complex were characterized using ISSR markers. On the basis of preliminary screening, 16 informative primers were selected for molecular profiling. All the ISSR primers generated distinct DNA amplification profiles, which could be used for identification of an individual taxon in the group. Primers with anchors at 5′ end generated the most polymorphic profiles. Based on their ability to distinguish between the taxa, expressed as resolving power (Rp), primer 885 showed the highest resolving power and 855 the least. A total of 332 reproducible bands were scored and all of them were polymorphic. In the cluster analysis of SWERTIA SPP., based on the band data, two main clusters were observed. The first consisted of S. ANGUSTIFOLIA and S. CORDATA; and the other of the remaining species of SWERTIA. Based on the similarity coefficient, S. LURIDA was closest to S. CHIRATA. The utility of ISSR markers for the authentication of commercial crude drug samples is also demonstrated. The results show that ISSR is an efficient and reliable marker system for the molecular authentication of medicinal plants as well as commercial crude drug samples.

Assessment of systemic interaction between Swertia chirata extract and its Bioactive constituents in rabbits.

Abstract: The plant Swertia chirata (Gentianaceae) is known for its multifarious medicinal value in the Indian system of medicine (Ayurveda). Its methanol extracts having antidiabetic activity contains mangiferin, amarogentin, amaroswerin, sweroside and swertiamarin as active constituents. The pharmacokinetics of mangiferin and amarogentin have been carried out after intravenous administration of pure standards and extract from S. chirata (CT) in rabbits to assess systemic interaction. The remaining three components were also monitored in plasma for pharmacokinetic estimation based on the ratio analysis method. Mangiferin was characterized by a relative low clearance ( approximately 0.14 L/h/kg) and a lesser volume of distribution ( approximately 0.15 L/kg), while amarogentin exhibited a rapid clearance ( approximately 2.62 L/h/kg) and wide distribution ( approximately 1.08 L/kg) from the systemic circulation. No significant difference was observed in pharmacokinetic parameters of mangiferin and amarogentin either administered alone or as CT formulation in rabbits.

Quantification of Swertiamarin from Whole Plant Powder of Swertia densiflora (Griscb.) Kashyap. Collected in Different Seasons

Abstract: In this paper, the comparative quantification of Swertiamarin from Swertia densiflora (Griscb.) Kashyap in different periods, in order to identify the best period for harvest is investigated. The plant powder was first extracted with methanol and then in chloroform. The final residue was reconstituted in methanol and used for quantitation. Chromatography was performed on silica gel 60 F 254 HPTLC plate, with ethyl acetate:methanol:water, 7.5:1.5:1.2 (v/v), as mobile phase. Quantitation was achieved by densitometric scanning at 244 nm (λmax) in reflectanceabsorbance mode. The response to swertiamarin was linear function of concentration over the range 30 to 100 µL mL -1 in the extract of Swertia densiflora (Griscb.) Kashyap. The amount of swertiamarin in Swertia densiflora (Griscb.) Kashyap. whole plant powder was found to be 2.94 mg g -1 in the month of March, 1.59 mg g -1 in October and 1.18 mg g -1 in December.

Determination of six active components in three species of genus Swertia by HPLC multiwavelength with detection

Abstract: OBJECTIVE To develop an HPLC method for the quantification of six active components in three species (Swertia davidi, S nervosa and S mussotii) METHOD The determination was performed on a Hypersil BDS colunm (4 6 mm x 200 mm, 5 microm) Acetonitrile and 05% phosphoric acid solution were used as the mobile phases with a gradient elution The flow rate was 10 mL x min(-1) The UV detection wavelength was at 240, 274, 325 and 334 nm The column oven temperature was at 25 degrees C RESULT Six components were separated commendably in 60 minutes The calibration curves of swertiamarin, gentiopicroside, norswertianolin, swertianolin, demethylbellidifolin and bellidifolin were in good linearity over the range of 0520-208, 0202-806, 0107-428, 0097-386, 0094-377, 0101-402 microg, respectively (r = 0999 9) The average recoveries were 987%, 981%, 983%, 988%, 981% and 986%, respectively, and the RSD were less than 30% (n = 6) CONCLUSION The method is accurate,simple and reproducible, and can be used to control the quality of Swertia

Swertia diluta extract and preparation method and use thereof

Abstract: The invention relates to a false Chinese swertia herb extract, a preparation method and an application thereof. The method takes false Chinese swertia herb as raw material, and the false Chinese swertia herb extract which contains more than 50 percent of secoiridoid compounds, more than 40 percent of single ingredient of swertiamain and more than 45 percent of three ingredients of chiratin, gentiopicroside, and the like, is obtained by using only one step of the macroporous absorption resin purification process after the extraction. The extract has significant effects on the protection, the prevention and the treatment of liver injury. The product has simple method and operation, high product yield, good repeatability and strong feasibility.

In vitro antimicrobial and anthelmintic activity of steam distillates of Hemidesmus indicus and Swertia chirata alone and in combination with Cow urine

Abstract: Essential oils are valuable natural products used as rawmaterials in many fields. This study reveals antimicrobial and anthelmintic activity of steam distillates of Hemidesmus indicus and Swertia chirata alone and in combination with Cow urine. The steam distillates were obtained by subjecting the powders of plants to distillation using simple distillation apparatus. The distillates obtained were tested for antibacterial activity, antifungal activity and anthelmintic activity. The results obtained revealed potential of the distillates against bacteria, fungi and wormtested. Significant result was seen in case of Hemidesmus distillate when compared to other distillates. Hemidesmus distillate alone was found to be superior to distillate fromHemidesmus-Cowurine combination. Swertia distillate was found to possess lesser activitywhen compared to combination.Among fungi tested, Aspergillus niger was found to be affected to more extent than other fungi. The results revealed the potential of steam distillates against bacteria, fungi and helminthes. The inhibitory effect of distillates could be attributed to active principles present.

Study on purification of swertiaman from Swertia pesudochinensis Hara. with macroporous adsorbent resin.

Abstract: Objective To explore the suitable type of macroporous adsorbent resin to purity swertiaman from Swertia pesudochinensis Hara., and determine the parameters and conditions for its purification procedures. Method Purification effect of 8 types of macroporous resins used to purify the Swertia pesudochinensis Hara. were inspected, with the content of swertiaman as index. Result HPD-100 type resin showed better effect than others, it had optimum adsorption and elution parameters with its dynamic saturated adsorption ratio was 70.48 mg/g. After eluted with 2 BV of distilled water, 4 BV 30% ethanol and collected, the content of swertiaman in the spray dried powder was 52.29%. Conclusion HPD-100 types macroporous resin showed a better comprehensive adsorption property and can be used to enrich and purify the swertiaman included active fraction of iridoid glycosides for industrialization.

Investigation on medicinal plant resources of Swertia in Yunnan province

Abstract: OBJECTIVE To explore the species and distribution of medicinal plants Swertia in Yunnan province, and provide scientific basis for sustainable utilization. METHOD Field investigation was carried out, specimens were collected, and literature and data were consulted. RESULT There were 35 species and 2 varieties (including 1 newly recorded species), most of them were used as medicinal plants, and an identification index was established. CONCLUSION The results provide reliable foundation for comprehensive utilization and in-depth study of Swertia in Yunnan province.

Anti-fever botanical composition and uses thereof

Abstract: According to at least one aspect of the present invention, a botanical anti-fever composition is provided. In at least one embodiment, the botanical composition comprises a therapeutically effective amount of admixture of parts or extracts of at least one plant species from each of genus Baptisia and genus Swertia. In at least one particular embodiment, the botanical composition contains Baptisia Tincoria. In at least another particular embodiment, the botanical composition contains Swertia Chirata.