Showing papers on "Trichoderma longibrachiatum published in 2018"

Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi

Abstract: An efficient biosynthesis process for the rapid production of nanoparticles would enable the development of a “microbial nanotechnology” for mass-scale production. In the present research, biological silver nanoparticle was synthesized extracellularly by using the fungus, Trichoderma longibrachiatum, where the cell filtrate of the fungus was used as a reducing and stabilizing agent in the process of nanoparticle synthesis. Different physical parameters such as fungal biomass concentration (1, 5, 10, 15, and 20 g), temperature (25, 28, and 33 °C), incubation time (0–120 h), and agitation (shaken or not shaken) were investigated, in order to determine the optimal conditions for nanoparticle biosynthesis. The stability and antifungal properties of the synthesized silver nanoparticles (AgNPs) were also determined. Data revealed that a combination of 10 g fungal biomass, a reaction temperature of 28 °C, a 72-h incubation time, and without shaking were the optimum conditions for the synthesis of the silver nanoparticles. Visual observation of brown color is an indication of silver nanoparticle production. UV–vis spectroscopy showed maximum absorption at 385 nm with the optimum conditions. Transmission electron microscopy (TEM) revealed the formation of monodispersed spherical shape with a mean diameter of 10 nm. Fourier transformation infrared (FTIR) showed bands at1634.92 and 3269.31 cm−1. Dynamic light scattering (DLS) supported that the Z-average size was 24.43 and 0.420 PdI value. Zeta potential showed − 19.7 mV with a single peak. The AgNPs synthesized through this biosystem approach were relatively stable up to 2 months after synthesis. The use of AgNPs as antifungal led to significant reductions in the number of forming colonies for many plant pathogenic fungi, with efficiencies reaching up to 90% against Fusarium verticillioides, Fusarium moniliforme, Penicillium brevicompactum, Helminthosporium oryzae, and Pyricularia grisea. However, further research should be carried out in order to determine the toxic effect of AgNPs before mass production and use of agricultural applications.

Valorization of Corn-Cob by Fungal Isolates for Production of Xylanase in Submerged and Solid State Fermentation Media and Potential Biotechnological Applications

Abstract: The increasing awareness of importance of xylanases in various industrial processes has led to intensive research in effective production of xylanases. In this study, eight fungal strains were screened for the production of xylanases in submerged (SmF) and solid state fermentations (SSF) in corncob-based media. Positive results of reddish orange halo-zones of hydrolysis were obtained for qualitative screening. In SmF, xylanase activity from Aspergillus fumigatus SD5A yielded highest activity (50.55 U/ml) in 168 h, while Aspergillus flavus SD4A, A. fumigatus L1, Fusarium solani SD3C, Aspergillus niger L3, Trichoderma longibrachiatum L2, Botryodiplodia sp. L5 and A. flavus L4, showed xylanase activities ranging from 10.38 to 44.81 U/ml. In SSF, xylanase activities ranged from 12.30 to 48.63 U/g in 120 h, with the highest activity obtained from A. fumigatus L1. Optimum temperatures for xylanases obtained in SmF ranged from 55 to 70 °C, while that of SSF ranged from 50 to 70 °C. Also, the optimum pH for xylanases obtained in both SmF and SSF ranged from 5.0 to 7.0. All the fungi did not produce aflatoxin on neutral red desiccated coconut agar. The fungal xylanases improved dough rising of bread and clarification of orange juice by 1.87–2.2-folds and 58.12–74.22% respectively. The results reported here showed that the range of characteristics exhibited by the xylanases could make them widely applicable in various industries, and also an important way for the valorization of agricultural waste.

The Hydrophobin HYTLO1 Secreted by the Biocontrol Fungus Trichoderma longibrachiatum Triggers a NAADP-Mediated Calcium Signalling Pathway in Lotus japonicus .

Abstract: Trichoderma filamentous fungi are increasingly used as biocontrol agents and plant biostimulants. Growing evidence indicates that part of the beneficial effects is mediated by the activity of fungal metabolites on the plant host. We have investigated the mechanism of plant perception of HYTLO1, a hydrophobin abundantly secreted by Trichoderma longibrachiatum, which may play an important role in the early stages of the plant-fungus interaction. Aequorin-expressing Lotus japonicus suspension cell cultures responded to HYTLO1 with a rapid cytosolic Ca2+ increase that dissipated within 30 min, followed by the activation of the defence-related genes MPK3, WRK33, and CP450. The Ca2+-dependence of these gene expression was demonstrated by using the extracellular Ca2+ chelator EGTA and Ned-19, a potent inhibitor of the nicotinic acid adenine dinucleotide phosphate (NAADP) receptor in animal cells, which effectively blocked the HYTLO1-induced Ca2+ elevation. Immunocytochemical analyses showed the localization of the fungal hydrophobin at the plant cell surface, where it forms a protein film covering the plant cell wall. Our data demonstrate the Ca2+-mediated perception by plant cells of a key metabolite secreted by a biocontrol fungus, and provide the first evidence of the involvement of NAADP-gated Ca2+ release in a signalling pathway triggered by a biotic stimulus.

Thermodynamics characterization and potential textile applications of Trichoderma longibrachiatum KT693225 xylanase

Abstract: Our study was a trial to participate in solving two main problems namely, environmental pollution resulting from accumulation and bad disposal of agro-industrial wastes, and high cost of industrial xylanase enzyme production. This was achieved through successful xylanase production by solid-state fermentation of low cost disposable agricultural wastes by marine fungal isolate Trichoderma longibrachiatum KT693225. The highest xylanase production 7.13 ± 0.11 U ml−1 was obtained utilizing rice straw (RS) waste after 7days of fermentation. Xylanase was purified by fractional precipitation with ethanol causing 4.24-fold purification. The 75% ethanol fraction was rich in cellulase, pectinase and α-amylase enzymes beside xylanase. The maximal xylanase activity was obtained at 60 °C, pH 5% and 2.5% xylan concentration. The Km and Vmax were calculated to be 20 mg ml−1 and 20 µmol min−1 ml−1, respectively. The thermostability of T.longibrachiatum KT693225 xylanase was indicated by low Ea (activation energy)and high Ed (energy of denaturation). High T1/2 (half life), D-value (decimal reduction time), ΔH° (enthalpy), ΔG° (free energy) and low Kd (denaturation rate constant), ΔS° (entropy) values at 70 °C emphasized high T.longibrachiatum KT693225 xylanase stability. T.longibrachiatum KT693225 xylanase showed high effectiveness at several textile wet-processing stages including desizing, bioscouring and biofinishing of cellulosic fabrics without adding any additives. These findings in this study have great implications for the future applications of xylanases.

First case of proven invasive pulmonary infection due to Trichoderma longibrachiatum in a neutropenic patient with acute leukemia.

Abstract: Trichoderma species are saprophytic filamentous fungi that can be found all over the word. These fungi show increasing medical importance as opportunistic human pathogens, particularly in immunocompromised patients. Invasive infections due to Trichoderma are rare and definitive diagnosis is complex to achieve because of the lack of specific diagnosis tools. We report in this work the first proven case of invasive pulmonary infection due to T. longibrachiatum in a 69-year-old white male with hematologic malignancy. The patient was successfully treated initially with voriconazole alone followed by a combination of voriconazole and caspofungine.

Screening of a Novel Glycoside Hydrolase Family 51 α-L-Arabinofuranosidase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Characterization

Abstract: Paenibacillus polymyxa exhibits remarkable hemicellulolytic activity. In the present study, 13 hemicellulose-degrading enzymes were identified from the secreted proteome of P. polymyxa KF-1 by liquid chromatography-tandem mass spectrometry analysis. α-L-arabinofuranosidase is an important member of hemicellulose-degrading enzymes. A novel α-L-arabinofuranosidase (PpAbf51b), belonging to glycoside hydrolase family 51, was identified from P. polymyxa. Recombinant PpAbf51b was produced in Escherichia coli BL21 (DE3) and was found to be a tetramer using gel filtration chromatography. PpAbf51b hydrolyzed neutral arabinose-containing polysaccharides, including sugar beet arabinan, linear-1,5-α-L-arabinan, and wheat arabinoxylan, with L-arabinose as the main product. The products from hydrolysis indicate that PpAbf51b functions as an exo-α-L-arabinofuranosidase. Combining PpAbf51b and Trichoderma longibrachiatum endo-1,4-xylanase produced significant synergistic effects for the degradation of wheat arabinoxylan. The α-L-arabinofuranosidase identified from the secretome of P. polymyxa KF-1 is potentially suitable for application in biotechnological industries.

Internal transcribed spacers (ITS) based identification of Trichoderma isolates and biocontrol activity against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita

Abstract: Ten Trichoderma isolates were isolated from different locations in Egypt Amplification and sequencing of internal transcribed spacers (ITS) was employed to identify Trichoderma isolates that exhibited from 99 to 100% identity with three species of Trichoderma: Trichoderma harzianum, Trichoderma asperellum and Trichoderma longibrachiatum The biocontrol activity of Trichoderma isolates against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita was tested in vitro and under greenhouse conditions The results show that the isolate Th2 (T harzianum) gave the best antagonism against M phaseolina and A niger with inhibition rates of 7285 and 6428%, respectively Moreover, the isolate Ta1 (T asperellum) was the best efficient isolate in reduction of each second stage juveniles (J2), number of galls, egg masses and females per root system with 9033, 9059, 9006 and 8550%, respectively Treatment with Trichoderma isolates improved tomato growth parameters (root length, plant height, roots and shoots fresh weight and shoots dry weight) Key words: Trichoderma, internal transcribed spacers (ITS), antagonism, Macrophomina phaseolina, Aspergillus niger, Meloidogyne incognita

Efektivitas Beberapa Isolat Trichoderma Sp. Terhadap Keterjadian Penyakit Bulai yang Disebabkan oleh Peronosclerospora maydis dan Pertumbuhan Tanaman Jagung (Zea mays)

Abstract: Corn (Zea mays) is one of the important cereals crops as the source of food, animals feed or energy. The corn produces has decreased by year to year. One of the factors that decreased the corn produces is downy mildew. Downy mildew desease caused by Peronosclerospora maydis fungi that decreased the production until 90%. Application of antagonistic fungi is one of the alternative control. Trichoderma spp. is one of the antagonistic fungi that can suppress growth of another fungus. Trichoderma spp. is saprofit soil microorganism that naturally attacks pathogen fungi and has benefits for plant growth. This research was aimed to know the effect of some isolates of Trichoderma spp. for diseases of downy mildew and the effect for the plant growth. Treatments were prepared with Randomized Complete Block (RCB) with 7 treatments and 4 repetitions. The treatments consist are P0 (control), P1 (application Trichoderma viride isolate GDR), P2 (application Trichoderma harzianum isolate TRJ), P3 (application Trichoderma harzianum isolate TGN), P4 (application Trichoderma viride isolate NTF), P5 (application Trichoderma longibrachiatum isolate GRP) and P6 (application Trichoderma viride isolate KLN). The results of this research were Trichoderma viride isolate GDR, Trichoderma harzianum isolate TGN, Trichoderma viride isolate NTF and Trichoderma viride isolate KLN can be suppressed the disease incidence of downy mildew, but unaffected for the plants growth.

Preparation method and application of water dispersible granules containing Trichoderma longibrachiatum T6 as biocontrol bacterium

Abstract: The invention discloses a preparation method for successfully developing water dispersible granules containing Trichoderma longibrachiatum T6 by utilizing an antagonistic bacterium, namely, Trichoderma longibrachiatum T6 separated from soil as a research object, optimizing a culture medium formula and screening auxiliary types, and storage stability of the water dispersible granules is measured. The water dispersible granules in the formula comprise components as follows: a master batch prepared from grass peat, vermiculite and cow dung as well as an auxiliary prepared from carboxymethyl cellulose, calcium carbonate, polyvinyl pyrrolidone, soluble starch, attapulgite and lauryl sodium sulfate. Tests prove that the water dispersible granules containing Trichoderma longibrachiatum T6 as thebiocontrol bacterium have remarkable growth-promoting effect on cucumbers, tomatoes, hot peppers, oilseed rapes and Chinese cabbages and have obvious biological control effect on occurrence and harm of cucumber rhizoctonia rot, rhizoctonia rot of peppers and phoma root rot of colza.

Microbial biocontrol preparation

Abstract: The invention provides a microbial biocontrol preparation. The microbial biocontrol preparation is a liquid and comprises active ingredients of Trichoderma longibrachiatum and its fermentation metabolites, bacillus amyloliquefaciens and its fermentation metabolites, Rhodopseudomonas palustris and its fermentation metabolites, berberine and chitosan. The microbial biocontrol preparation has a berberine concentration of 0.1 to 0.8 wt% and a chitosan concentration of 0.5-1wt%. The total viable count of the Trichoderma longibrachiatum, bacillus amyloliquefaciens and Rhodopseudomonas palustris is greater than or equal to 5*10 cfu/ml. The active ingredients belong to different action mechanisms. Under the synergistic effect of the active ingredients, the microbial biocontrol preparation has good prevention effects on tea grey blight. The active ingredients are environmentally friendly and non-toxic and haves pharmacological and fertilizer dual biological regulation functions.

Strain of trichoderma longibrachiatum tw-14-220 filamentous fungus - producer of cellulases, beta-glucanases and xylanases for feed production and a method for obtaining a feed complex enzyme preparation

Abstract: FIELD: biotechnology.SUBSTANCE: strain of Trichoderma Longibrachiatum TW-14-220T filamentous fungus – a producer of a complex of enzymes of endoglucanases, beta-glucanases and xylanases destroying non-starch polysaccharides, and a method for producing a feeding enzyme preparation using a Trichoderma longibrachiatum TW-14-220T filamentous fungus strain. Method for producing the feeding complex enzyme preparation involves deep cultivation of the Trichoderma Longibrachiatum TW-14-220T fungus strain, deposited in the Russian National Collection of Microorganisms under the registration number of VKM F-4642D on suitable fermentation media, followed by centrifugation, filtration, ultrafiltration and lyophilization of the culture fluid.EFFECT: group of inventions makes it possible to increase the degree of biodegradation of non-starch polysaccharides.2 cl, 4 tbl, 3 ex

Compound microbial agent and preparation method and application thereof

Abstract: The invention discloses a compound microbial agent. The compound microbial agent is prepared from nitrophilous rhizobium ATCC 35116 culture, trichoderma longibrachiatum AS3.1029 culture and acremoniumATCC 48379 culture. By culture solution prepared by mixed culture of the nitrophilous rhizobium ATCC 35116 culture, the trichoderma longibrachiatum AS3.1029 culture and the acremonium ATCC 48379 culture, the compound microbial agent has an excellent treatment effect on sewage mineral fertilizers, various organics, colloids, macromolecular substances such as sugars and organic acids, and the like,and accordingly effectiveness in domestic sewage purification treatment is realized.

Trichoderma longibrachiatum strain and application thereof

Abstract: The invention discloses a trichoderma longibrachiatum strain and application thereof. The strain is named as trichoderma longibrachiatum TL1, and is collected in the China Culture for Type Culture Collection on April 10, 2017, with the collection number of CCTCC No. M2017184. The strain has a relatively strong inhibitory effect on pathogenic fungi causing clubroot of tuber mustard, especially hasa significant effect of controlling the clubroot of Fuling tuber mustard; after a trichoderma longibrachiatum TL1 fermentation solution is used for soaking young seedlings of the tuber mustard, the disease incidence of the tuber mustard is reduced by 26% and the yield thereof is increased by 31%, so that the strain has good economic efficiency; in addition, a fermentation technology of the strainis simple and stable, so that the strain has a great agricultural application prospect.

Trichoderma longibrachiatum strain and application thereof

Abstract: The invention discloses a trichoderma longibrachiatum strain and an application thereof. The trichoderma longibrachiatum T61 provided by the invention has cadmium reducing and bio-control functions; the strain is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M2017447, and nucleotide sequence of a 28Sr RNA gene is shown as SEQ ID No.1. Based upon experiments, it is proved that the trichoderma longibrachiatum T61 provided by the invention can purify cadmium of an exchangeable form in soil so that the content of the cadmium is obviously reduced; the trichoderma longibrachiatum T61 is quite strong in tolerance to cadmium, low in disturbance to soil and relatively strong in bioremediation function; meanwhile, crops, applied by the trichoderma longibrachiatum T61, are obvious in effects of resisting diseases and pests, and meanwhile, biomass content in the soil can be obviously improved and physiochemical properties of the soil can be improved. Therefore, the trichoderma longibrachiatum T61 provided by the invention can be applied to remediation of heavy metal contaminated soil, improvement of the biomass content of the soil, improvement of the physiochemical properties of the soil as well as to preparation of related products.

Trichoderma longibrachiatum with broad-spectrum antibacterial performance and application thereof

Abstract: The invention provides a strain of Trichoderma longibrachiatum MF-3, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 23, 2018,and the preservation number is CGMCC NO 15665 The strain has prominent broad-spectrum antibacterial ability, especially has a prominent inhibitory effect on rhizoctonia solani, fusarium oxysporum and fusarium graminearum The strain can be used for effectively reducing the use of chemical agents in the aspects of inhibiting various soil-borne diseases, and has huge application potential in theaspects of producing pollution-free food and improving ecological environment