A
Akinobu Nakamura
Researcher at Nagoya Institute of Technology
Publications - 27
Citations - 364
Akinobu Nakamura is an academic researcher from Nagoya Institute of Technology. The author has contributed to research in topics: Sewage treatment & Cell signaling. The author has an hindex of 9, co-authored 24 publications receiving 244 citations. Previous affiliations of Akinobu Nakamura include National Institutes of Natural Sciences, Japan & Nagaoka University of Technology.
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Journal ArticleDOI
Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging
Akinobu Nakamura,Kazumasa Takigawa,Yasutaka Kurishita,Keiko Kuwata,Manabu Ishida,Yasushi Shimoda,Itaru Hamachi,Shinya Tsukiji +7 more
TL;DR: This work reports a general strategy to create small-molecule fluorescent probes for the nucleus in living cells based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest.
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Synthetic Self-Localizing Ligands That Control the Spatial Location of Proteins in Living Cells
Manabu Ishida,Hideaki Watanabe,Kazumasa Takigawa,Yasutaka Kurishita,Choji Oki,Akinobu Nakamura,Itaru Hamachi,Shinya Tsukiji +7 more
TL;DR: It is shown that SLLs bind their target proteins and relocate (tether) them rapidly from the cytoplasm to their targeting sites, thus serving as synthetic protein translocators, thus opening a new direction in the design of small-molecule tools or drugs for cell regulation.
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Bacteroides luti sp. nov., an anaerobic, cellulolytic and xylanolytic bacterium isolated from methanogenic sludge.
TL;DR: The genetic and phenotypic properties suggest that strain UasXn-3T represents a novel species, and the name Bacteroides luti sp.
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Ratiometric fluorescence imaging of nuclear pH in living cells using Hoechst-tagged fluorescein.
Akinobu Nakamura,Shinya Tsukiji +1 more
TL;DR: Hoechst-tagged fluorescein (hoeFL), which was previously developed as a green fluorescent DNA-staining probe, can be used for this purpose and was applicable to visualize the intranuclear pH of nigericin-treated and intact living human cells by ratiometric fluorescence imaging.
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Engineering Orthogonal, Plasma Membrane-Specific SLIPT Systems for Multiplexed Chemical Control of Signaling Pathways in Living Single Cells.
Akinobu Nakamura,Choji Oki,Kenya Kato,Satoko Fujinuma,Gembu Maryu,Gembu Maryu,Keiko Kuwata,Tatsuyuki Yoshii,Tatsuyuki Yoshii,Michiyuki Matsuda,Kazuhiro Aoki,Kazuhiro Aoki,Shinya Tsukiji +12 more
TL;DR: Orthogonal PM-specific SLIPT systems provide a powerful new platform for multiplexed chemical signal control in living single cells, offering new opportunities for dissecting cell signaling networks and synthetic cell manipulation.