Alan X. Ji

TL;DR: A computational platform that integrates the analysis of copy number variations and somatic mutations and unravels the landscape of in-frame gene fusions in glioblastoma provides insights into the pathogenesis of gliOBlastoma and highlights new targets for therapeutic intervention.

TL;DR: This work uses isothermal titration calorimetry to directly demonstrate that several of the disease mutations in the KLHL3 BTB-BACK domains disrupt the association with Cul3, and an extended model of the dimeric CRL3 complex places the two E2 binding sites in a suprafacial arrangement with respect to the presumed substrate-binding sites.

TL;DR: The 5:5 assembly of KCTD9/Cul3 complexes by cryo-electron microscopy is confirmed and the molecular rationale for BTB-mediated Cul3 binding specificity in the KCTd family is provided.

TL;DR: In order to investigate the regulatory mechanism of (de)phosphorylation of ICDH by AceK, AceK was successfully cocrystallized in complex with its intact protein substrate, I CDH, in the presence of ATP.