David Ayares

TL;DR: The successful production of cloned piglets from a cultured adult somatic cell population using a new nuclear transfer procedure is reported and the methodology used for embryo reconstruction in each of these species is essentially similar.

TL;DR: The piglets carrying a point mutation in the α1,3GT gene hold significant value, as they would allow production of α1-3Gal-deficient pigs free of antibiotic-resistance genes and thus have the potential to make a safer product for human use.

TL;DR: The disruption of one allele of the pig α1,3GT gene in both male and female porcine primary fetal fibroblasts is reported, causing hyperacute rejection in pig-to-human xenotransplantation and producing cloned female piglets of normal weight and apparently healthy.

TL;DR: Genetic modifications combined with the treatment regimen tested here consistently prevent humoral rejection and systemic coagulation pathway dysregulation, sustaining long-term cardiac xenograft survival beyond 900 days.

TL;DR: It is shown that α1,3-galactosyltransferase-knockout pig hearts that express human CD46 and thrombomodulin require non-ischaemic preservation with continuous perfusion and post-transplantation growth control to ensure long-term orthotopic function of the xenograft in baboons, the most stringent preclinical xenotransplantation model.