Amanda C. Heppell-Parton

TL;DR: A strategy for chromosome engineering in embryonic stem (ES) cells that relies on sequential gene targeting and Cre–loxP site–specific recombination and will allow the design of a variety of chromosome rearrangements that can be selected and verified in ES cells or activated in ES cell–derived mice.

TL;DR: This is the first demonstration of how a complete set of mouse chromosome paints can be produced, separated using flow cytometry into 18 distinguishable peaks.

TL;DR: The 190k protein detected immunologically is likely to be the protein, encoded by the MRP gene, which becomes overexpressed in these cells as a consequence of chromosomal amplification and fragmentation.

TL;DR: A physical map of the region homozygously deleted in the U2020 cell line at 3p12 is constructed, including the location of putative CpG islands and a new gene (DUTT1) is identified and cloned and probes from this gene are used to detect two other homozygous deletions occurring in lung and breast carcinomas.

TL;DR: The isolation and characterization of 39 new single‐copy probes saturating a submicroscopic homozygous deletion detected in the DNA of the small cell lung cancer (SCLC) cell line U2020 are reported, based on the number of probes in the deletion and the probe density.