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Andrew Gordus
Researcher at Johns Hopkins University
Publications - 28
Citations - 5473
Andrew Gordus is an academic researcher from Johns Hopkins University. The author has contributed to research in topics: Receptor tyrosine kinase & Tyrosine phosphorylation. The author has an hindex of 18, co-authored 24 publications receiving 4675 citations. Previous affiliations of Andrew Gordus include Harvard University & University of Texas Southwestern Medical Center.
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Journal ArticleDOI
Optimization of a GCaMP calcium indicator for neural activity imaging.
Jasper Akerboom,Tsai Wen Chen,Trevor J. Wardill,Lin Tian,Lin Tian,Jonathan S. Marvin,Sevinç Mutlu,Sevinç Mutlu,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Federico Esposti,Bart G. Borghuis,Bart G. Borghuis,Xiaonan Richard Sun,Andrew Gordus,Michael B. Orger,Michael B. Orger,Ruben Portugues,Florian Engert,John J. Macklin,Alessandro Filosa,Aman Aggarwal,Aman Aggarwal,Rex Kerr,Ryousuke Takagi,Sebastian Kracun,Eiji Shigetomi,Baljit S. Khakh,Herwig Baier,Leon Lagnado,Samuel S.-H. Wang,Cornelia I. Bargmann,Bruce E. Kimmel,Vivek Jayaraman,Karel Svoboda,Douglas S. Kim,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +39 more
TL;DR: GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.
Journal ArticleDOI
An optimized fluorescent probe for visualizing glutamate neurotransmission
Jonathan S. Marvin,Bart G. Borghuis,Bart G. Borghuis,Lin Tian,Lin Tian,Joseph Cichon,Mark T. Harnett,Jasper Akerboom,Andrew Gordus,Sabine L. Renninger,Tsai Wen Chen,Cornelia I. Bargmann,Michael B. Orger,Eric R. Schreiter,Jonathan B. Demb,Wen-Biao Gan,S. Andrew Hires,Loren L. Looger +17 more
TL;DR: An intensity-based glutamate-sensing fluorescent reporter with signal-to-noise ratio and kinetics appropriate for in vivo imaging is described and its utility for visualizing glutamate release by neurons and astrocytes in increasingly intact neurological systems is validated.
Journal ArticleDOI
Sensitive red protein calcium indicators for imaging neural activity
Hod Dana,Boaz Mohar,Boaz Mohar,Yi Sun,Sujatha Narayan,Andrew Gordus,Jeremy P. Hasseman,Getahun Tsegaye,Graham T Holt,Amy Hu,Deepika Walpita,Ronak Patel,John J. Macklin,Cornelia I. Bargmann,Misha B. Ahrens,Eric R. Schreiter,Vivek Jayaraman,Loren L. Looger,Karel Svoboda,Douglas S. Kim +19 more
TL;DR: Improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a) are presented, with sensitivity comparable to GCaMP6, to facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.
Journal ArticleDOI
A quantitative protein interaction network for the ErbB receptors using protein microarrays
TL;DR: The extent to which promiscuity changes with protein concentration may contribute to the oncogenic potential of receptor tyrosine kinases, and perhaps other signalling proteins as well.
Journal ArticleDOI
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Jasper Akerboom,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Lin Tian,Lin Tian,Sebastian Wabnig,Matthias Prigge,Johan Tolö,Andrew Gordus,Michael B. Orger,Michael B. Orger,Kristen E. Severi,John J. Macklin,Ronak Patel,Stefan R. Pulver,Trevor J. Wardill,Trevor J. Wardill,Elisabeth Fischer,Christina Schüler,Tsai Wen Chen,Karen S. Sarkisyan,Jonathan S. Marvin,Cornelia I. Bargmann,Douglas S. Kim,Sebastian Kügler,Leon Lagnado,Peter Hegemann,Alexander Gottschalk,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +31 more
TL;DR: Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes.