A
April Allen
Researcher at Fred Hutchinson Cancer Research Center
Publications - 7
Citations - 7896
April Allen is an academic researcher from Fred Hutchinson Cancer Research Center. The author has contributed to research in topics: Cancer & microRNA. The author has an hindex of 7, co-authored 7 publications receiving 7230 citations. Previous affiliations of April Allen include University of Washington.
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Journal ArticleDOI
Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
TL;DR: It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.
Journal ArticleDOI
Epigenetic silencing of the intronic microRNA hsa-miR-342 and its host gene EVL in colorectal cancer.
William M. Grady,Rachael K. Parkin,Patrick S. Mitchell,Jun Ho Lee,Young Ho Kim,Karen D. Tsuchiya,Mary Kay Washington,C Paraskeva,James K V Willson,Andrew M. Kaz,Evan M. Kroh,April Allen,Brian R. Fritz,Sanford D. Markowitz,Muneesh Tewari +14 more
TL;DR: Reconstitution of hsa-miR-342 in the colorectal cancer cell line HT-29 induced apoptosis, suggesting that this microRNA could function as a proapoptotic tumor suppressor and support a novel mechanism for silencing intronic microRNAs in cancer by epigenetic alterations of cognate host genes.
Journal ArticleDOI
Spectral Analysis of Multiplex Raman Probe Signatures
Barry R. Lutz,Claire Dentinger,Lienchi N. Nguyen,Lei Sun,Jingwu Zhang,April Allen,Selena Chan,Beatrice S. Knudsen +7 more
TL;DR: A novel spectral fitting method is reported that exploits the entire spectral signature to quantitatively extract individual probe signals from multiplex spectra and significantly expands the potential of Raman probes for quantitative in vivo and ex vivo multiplex analysis.
Journal ArticleDOI
Raman Nanoparticle Probes for Antibody-based Protein Detection in Tissues
Barry R. Lutz,Claire Dentinger,Lei Sun,Lienchi Nguyen,Jingwu Zhang,A. J. Chmura,April Allen,Selena Chan,Beatrice S. Knudsen +8 more
TL;DR: Overall, COIN and Alexa detection reagents possess similar performance with FFPE tissues, supporting the further development of Raman probes for this application.
Journal ArticleDOI
Evaluation of the branched-chain DNA assay for measurement of RNA in formalin-fixed tissues
Beatrice S. Knudsen,Beatrice S. Knudsen,April Allen,Dale McLerran,Robert L. Vessella,Jonathan Karademos,Joan Davies,Botoul Maqsodi,Gary K. McMaster,Alan R. Kristal +9 more
TL;DR: The branched-chain DNA (bDNA) assay QuantiGene Reagent System is considerably more reliable, reproducible, and sensitive than qPCR, providing an alternative method for the measurement of gene expression in FFPE tissues.