Arndt Brachat

TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.

TL;DR: A dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA is constructed and tested, and some kanMX modules are flanked by 470 bp direct repeats, promoting in vivo excision with frequencies of 10–3–10–4.

TL;DR: GFP reporters consist of wild‐type GFP or GFP‐S65T coding sequences, lacking the ATG, fused to the S. cerevisiae ADH1 terminator and PCR‐synthesized 2·4 kb‐long double modules flanked by 40 bp‐long guide sequences were successfully targeted to the carboxy‐terminus of a number of S. Cerevisiae genes.

TL;DR: Using an approach combining genetic and biochemical techniques, it is shown that the assembly of the lipid-linked core oligosaccharide in the lumen of the endoplasmic reticulum occurs in a stepwise fashion.

TL;DR: Time-lapse microscopy of cnm67Delta1 cells with green fluorescent protein (GFP)-labeled nuclei indicated that CNM67 is dispensable for nuclear migration (congression) and nuclear fusion during conjugation, in agreement with previous data, indicating that cytoplasmic microtubules are organized by the half-bridge during mating.