Brian Wigdahl

TL;DR: In this paper, a review examines the strengths, limitations, and origins of the different classes of off-target cleavage detection systems including anchored primer enrichment (GUIDE-seq), in situ detection (BLISS), in vitro selection libraries (CIRCLE-seq) and in vitro genomic DNA digestion (Digenome-seq and SITE-Seq).

TL;DR: Electrophoretic mobility shift analyses utilizing double-stranded oligonucleotides homologous to each of the 21 bp repeats and nuclear extracts derived from selected cell lines of lymphocytic, neuronal, and glial origin suggest that the differential binding of cellular factors may play a role in basal as well as Tax-mediated LTR-directed transcription within cell populations of either immune or nervous system origin.

TL;DR: Efficient drug delivery to breastfeeding infants is achievable using the NSDS, and greater than 99% reduction of cell-associated HIV infectivity was achieved in the first 10 ml of milk.

TL;DR: The substitution of BVDU with 9-[(2-hydoxyethoxy)methyl]guanine (acyclovir; ACV) during a combined treatment with IFN-alpha inhibited HSV-1 replication and established in vitro virus latency that could be maintained for a longer period after inhibitor removal and a continued incubation at 37 degrees C.

TL;DR: The human immunodeficiency virus type 1 (HIV‐1) transactivator of transcription (Tat) protein functions both intracellularly and extracellularly, and its effects have been examined in HIV‐1‐associated neurocognitive disorders (HAND).