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Bunsei Kawakami
Researcher at Toyobo
Publications - 34
Citations - 1639
Bunsei Kawakami is an academic researcher from Toyobo. The author has contributed to research in topics: Gene & Restriction enzyme. The author has an hindex of 12, co-authored 34 publications receiving 1550 citations.
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Journal ArticleDOI
Complete sequencing and characterization of 21,243 full-length human cDNAs
Toshio Ota,Yutaka Suzuki,Tetsuo Nishikawa,Tetsuji Otsuki,Tomoyasu Sugiyama,Ryotaro Irie,Ai Wakamatsu,Koji Hayashi,Hiroyuki Sato,Keiichi Nagai,Kouichi Kimura,Hiroshi Makita,Mitsuo Sekine,Masaya Obayashi,Tatsunari Nishi,Toshikazu Shibahara,Toshihiro Tanaka,Shizuko Ishii,Junichi Yamamoto,Kaoru Saito,Yuri Kawai,Yuko Isono,Yoshitaka Nakamura,Kenji Nagahari,Katsuhiko Murakami,Tomohiro Yasuda,Takao Iwayanagi,Masako Wagatsuma,Akiko Shiratori,Hiroaki Sudo,Takehiko Hosoiri,Yoshiko Kaku,Hiroyo Kodaira,Hiroshi Kondo,Masanori Sugawara,Makiko Takahashi,Katsuhiro Kanda,Takahide Yokoi,Takako Furuya,Emiko Kikkawa,Yuhi Omura,Kumi Abe,Kumiko Kamihara,Naoko Katsuta,Kazuomi Sato,Machiko Tanikawa,Makoto Yamazaki,Ken Ninomiya,Tadashi Ishibashi,Hiromichi Yamashita,Katsuji Murakawa,Kiyoshi Fujimori,Hiroyuki Tanai,Manabu Kimata,Motoji Watanabe,Susumu Hiraoka,Yoshiyuki Chiba,Shinichi Ishida,Yukio Ono,Sumiyo Takiguchi,Susumu Watanabe,Makoto Yosida,Tomoko Hotuta,Junko Kusano,Keiichi Kanehori,Asako Takahashi-Fujii,Hiroto Hara,Tomo-o Tanase,Yoshiko Nomura,Sakae Togiya,Fukuyo Komai,Reiko Hara,Kazuha Takeuchi,Miho Arita,Nobuyuki Imose,Kaoru Musashino,Hisatsugu Yuuki,Atsushi Oshima,Naokazu Sasaki,Satoshi Aotsuka,Yoko Yoshikawa,Hiroshi Matsunawa,Tatsuo Ichihara,Namiko Shiohata,Sanae Sano,Shogo Moriya,Hiroko Momiyama,Noriko Satoh,Sachiko Takami,Yuko Terashima,Osamu Suzuki,Satoshi Nakagawa,Akihiro Senoh,Hiroshi Mizoguchi,Yoshihiro Goto,Fumio Shimizu,Hirokazu Wakebe,Haretsugu Hishigaki,Takeshi Watanabe,Akio Sugiyama,Makoto Takemoto,Bunsei Kawakami,Masaaki Yamazaki,Koji Watanabe,Ayako Kumagai,Shoko Itakura,Yasuhito Fukuzumi,Yoshifumi Fujimori,Megumi Komiyama,Hiroyuki Tashiro,Akira Tanigami,Tsutomu Fujiwara,Toshihide Ono,Katsue Yamada,Yuka Fujii,Kouichi Ozaki,Maasa Hirao,Yoshihiro Ohmori,Ayako Kawabata,Takeshi Hikiji,Naoko Kobatake,Hiromi Inagaki,Yasuko Ikema,Sachiko Okamoto,Rie Okitani,Takuma Kawakami,Saori Noguchi,Tomoko Itoh,Keiko Shigeta,Tadashi Senba,Kyoka Matsumura,Yoshie Nakajima,Takae Mizuno,Misato Morinaga,Masahide Sasaki,Takushi Togashi,Masaaki Oyama,Hiroko Hata,Manabu Watanabe,Takami Komatsu,Junko Mizushima-Sugano,Tadashi Satoh,Tadashi Satoh,Yuko Shirai,Yukiko Takahashi,Kiyomi Nakagawa,Koji Okumura,Takahiro Nagase,Nobuo Nomura,Hisashi Kikuchi,Yasuhiko Masuho,Riu Yamashita,Kenta Nakai,Tetsushi Yada,Yusuke Nakamura,Osamu Ohara,Takao Isogai,Sumio Sugano,Sumio Sugano +158 more
TL;DR: There seems to be a slight bias against GC-rich transcripts in current gene prediction procedures in the “full-length long Japan” collection of sequenced human cDNAs.
Journal ArticleDOI
Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.
Naoki Goshima,Yoshifumi Kawamura,Akiko Fukumoto,Aya Miura,Reiko Honma,Ryohei Satoh,Ai Wakamatsu,Ai Wakamatsu,Junichi Yamamoto,Kouichi Kimura,Tetsuo Nishikawa,Taichi Andoh,Yuki Iida,Kumiko Ishikawa,Emi Ito,Naoko Kagawa,Chie Kaminaga,Keiichi Kanehori,Bunsei Kawakami,Kiyokazu Kenmochi,Rie Kimura,Miki Kobayashi,Toshihiro Kuroita,Hisashi Kuwayama,Yukio Maruyama,Kiyoshi Matsuo,Kazuyoshi Minami,Mariko Mitsubori,Masatoshi Mori,Riyo Morishita,Atsushi Murase,Akira Nishikawa,Shigemichi Nishikawa,Toshihiko Okamoto,Noriko Sakagami,Yutaka Sakamoto,Yukari Sasaki,Tomoe Seki,Saki Sono,Akio Sugiyama,Tsuyoshi Sumiya,Tomoko Takayama,Yukiko Takayama,Hiroyuki Takeda,Takushi Togashi,Kazuhide Yahata,Hiroko Yamada,Yuka Yanagisawa,Yaeta Endo,Fumio Imamoto,Yasutomo Kisu,Shigeo Tanaka,Takao Isogai,Jun-ichi Imai,Shinya Watanabe,Nobuo Nomura +55 more
TL;DR: A foundation for simple and efficient production of human proteins using the versatile Gateway vector system is prepared and several cytokines containing disulfide bonds were produced in an active form in a nonreducing wheat germ cell-free expression system.
Journal ArticleDOI
Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5′-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi
Ken Ishikawa,Miki Watanabe,Toshihiro Kuroita,Ikuo Uchiyama,Janusz M. Bujnicki,Bunsei Kawakami,Masaru Tanokura,Ichizo Kobayashi +7 more
TL;DR: A novel plant-based cell-free translation procedure that bypasses the toxicity of these enzymes is used and two deoxyribonucleases are identified, including a novel restriction enzyme-generated terminus that has a novel 3D structure.
Journal ArticleDOI
Development of a novel method for operating magnetic particles, Magtration Technology, and its use for automating nucleic acid purification
Kimimichi Obata,Osamu Segawa,Mitsuru Yakabe,Yoshikazu Ishida,Toshihiro Kuroita,Katsunori Ikeda,Bunsei Kawakami,Yoshihisa Kawamura,Masafumi Yohda,Tadashi Matsunaga,Hideji Tajima +10 more
TL;DR: Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction and purified total DNA, total RNA and plasmids at an efficiency comparable to that of manual methods.
Journal ArticleDOI
Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique
Motoki Kyo,Tae Yamamoto,Hozumi Motohashi,Terue Kamiya,Toshihiro Kuroita,Toshiyuki Tanaka,James Douglas Engel,Bunsei Kawakami,Masayuki Yamamoto,Masayuki Yamamoto +9 more
TL;DR: It is found that a hydrophilic flexible spacer composed of the poly (ethylene glycol) moiety between DNA and alkanethiol self‐assembled monolayers on the surface is effective for preventing nonspecific adsorption and facilitating specific binding of MafG.