Journal ArticleDOI
Development of a novel method for operating magnetic particles, Magtration Technology, and its use for automating nucleic acid purification
Kimimichi Obata,Osamu Segawa,Mitsuru Yakabe,Yoshikazu Ishida,Toshihiro Kuroita,Katsunori Ikeda,Bunsei Kawakami,Yoshihisa Kawamura,Masafumi Yohda,Tadashi Matsunaga,Hideji Tajima +10 more
TLDR
Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction and purified total DNA, total RNA and plasmids at an efficiency comparable to that of manual methods.About:
This article is published in Journal of Bioscience and Bioengineering.The article was published on 2001-01-01. It has received 82 citations till now. The article focuses on the topics: Nucleic acid methods.read more
Citations
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Journal ArticleDOI
The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures
TL;DR: It is shown that a short incubation in nonionic aqueous solutions at temperatures above 70°C can efficiently break the interaction with biotin without denaturing the streptavidin tetramer and both molecules can therefore be re‐used.
Journal ArticleDOI
Magnetic particles for the separation and purification of nucleic acids
TL;DR: Traditional methods and methods based on magnetic particles for nucleic acid purification, and the synthesis of a variety of magnetic particles is presented in more detail.
Magnetic particles for separation and purification of nucleic acids
TL;DR: A review of magnetic particle-based methods for nucleic acid separation can be found in this paper, where the synthesis of a variety of magnetic particles is presented in more detail, as well as commercially available manual magnetic separators and automated systems for magnetic particle handling and liquid handling are mentioned.
Patent
System and method including analytical units
Brian D. Wilson,David L. Anderson,Matthew S. Davis,Matthew D. Erickson,Alan N. Johnson,Garrick A. Maurer,Michael J. Rosen,Mark F. Sauerburger,Daniel R. Schmidt,Joshua D. Wiltsie +9 more
TL;DR: In this paper, the authors describe a system for processing and analyzing biological fluids using assay cartridges which can be processed at different processing locations, such as a preparation location and an analysis location where samples can be analyzed.
Journal ArticleDOI
Diagnostics on acute myocardial infarction: Cardiac troponin biomarkers
Mohamad Faris Mohamad Fathil,M. K. Md Arshad,Subash C. B. Gopinath,Uda Hashim,R. Adzhri,Ramzan Mat Ayub,A. R. Ruslinda,A. H. Azman,M. Zaki,Thean-Hock Tang +9 more
TL;DR: Several ways of diagnostics have been formulated, which include enzyme-linked immunosorbent assay, chemiluminescent, fluoro-immunoassays, electrical detections, surface plasmon resonance, and colorimetric protein assay, which elucidates the strategies, methods and detection levels involved in these diagnostics on cardiac superior biomarkers.
References
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Journal ArticleDOI
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Journal ArticleDOI
Rapid and simple method for purification of nucleic acids.
R. Boom,C. J. A. Sol,M. M. M. Salimans,C. L. Jansen,P.M.E. Wertheim-van Dillen,J. van der Noordaa +5 more
TL;DR: A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.
Journal ArticleDOI
Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.
TL;DR: Using this concept, in vitro amplified plasmid DNA and chromosomal DNA were sequenced directly from single colonies and the system was found to be suitable for sequencing using both isotope- and fluorescent-labelled primers.